中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Involvement of Fas/FasL system in apoptotic signaling in testicular germ cells of male Wistar rats injected i.v. with microcystins

文献类型:期刊论文

作者Xiong, Qian1,2; Xie, Ping1; Li, Huiying1; Hao, Le1,2; Li, Guangyu1,2; Qiu, Tong1,2; Liu, Ying1,2
刊名TOXICON
出版日期2009-07-01
卷号54期号:1页码:1-7
ISSN号0041-0101
关键词Microcystin Fas/FasL Caspase Testis TUNEL staining Real-time PCR Western blot
通讯作者Xie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
中文摘要Previous studies have shown that gonads were the second target organ of microcystins (MCs), and that MCs exposure exerted obvious toxic effects on male reproductive system of mammals. However, relevant molecular evidences are still lacking. Fas-signaling pathway plays a key role in toxicant-induced germ cell apoptosis. This study was to evaluate the responses of Fas/FasL system related genes and proteins in testes of rats injected intravenously with MCs. Enhanced apoptosis of germ cells in the testes of MCs-treated rats was detected by the terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick end labeling (TUNEL) associated with up-regulation of the Fas/FasL system. Both Fas and FasL protein expression were induced evidently from I h post-injection, and this high expression level maintained throughout the experiment. In addition, the activation of caspase-8 and caspase-3 protein was also observed, which were indicators of apoptosis. These results suggested the likely involvement of Fas/FasL system in the MCs-induced germ cell apoptosis. It is also suggested that MCs can cause damage to Sertoli cells directly. (C) 2009 Elsevier Ltd. All rights reserved.
英文摘要Previous studies have shown that gonads were the second target organ of microcystins (MCs), and that MCs exposure exerted obvious toxic effects on male reproductive system of mammals. However, relevant molecular evidences are still lacking. Fas-signaling pathway plays a key role in toxicant-induced germ cell apoptosis. This study was to evaluate the responses of Fas/FasL system related genes and proteins in testes of rats injected intravenously with MCs. Enhanced apoptosis of germ cells in the testes of MCs-treated rats was detected by the terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick end labeling (TUNEL) associated with up-regulation of the Fas/FasL system. Both Fas and FasL protein expression were induced evidently from I h post-injection, and this high expression level maintained throughout the experiment. In addition, the activation of caspase-8 and caspase-3 protein was also observed, which were indicators of apoptosis. These results suggested the likely involvement of Fas/FasL system in the MCs-induced germ cell apoptosis. It is also suggested that MCs can cause damage to Sertoli cells directly. (C) 2009 Elsevier Ltd. All rights reserved.
学科主题Pharmacology & Pharmacy; Toxicology
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]Pharmacology & Pharmacy ; Toxicology
关键词[WOS]FAS LIGAND ; MONO-(2-ETHYLHEXYL) PHTHALATE ; TISSUE DISTRIBUTIONS ; REPRODUCTIVE-SYSTEM ; SEASONAL DYNAMICS ; SUBTROPICAL CHINA ; EUTROPHIC LAKE ; LARGE SHALLOW ; IN-VITRO ; LR
资助信息National Basic Research Program of China [2008CB418101]
收录类别SCI
语种英语
WOS记录号WOS:000267011600001
公开日期2010-10-13
源URL[http://ir.ihb.ac.cn/handle/152342/7692]  
专题水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文
作者单位1.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China
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GB/T 7714
Xiong, Qian,Xie, Ping,Li, Huiying,et al. Involvement of Fas/FasL system in apoptotic signaling in testicular germ cells of male Wistar rats injected i.v. with microcystins[J]. TOXICON,2009,54(1):1-7.
APA Xiong, Qian.,Xie, Ping.,Li, Huiying.,Hao, Le.,Li, Guangyu.,...&Liu, Ying.(2009).Involvement of Fas/FasL system in apoptotic signaling in testicular germ cells of male Wistar rats injected i.v. with microcystins.TOXICON,54(1),1-7.
MLA Xiong, Qian,et al."Involvement of Fas/FasL system in apoptotic signaling in testicular germ cells of male Wistar rats injected i.v. with microcystins".TOXICON 54.1(2009):1-7.

入库方式: OAI收割

来源:水生生物研究所

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