Involvement of Fas/FasL system in apoptotic signaling in testicular germ cells of male Wistar rats injected i.v. with microcystins
文献类型:期刊论文
作者 | Xiong, Qian1,2; Xie, Ping1; Li, Huiying1; Hao, Le1,2; Li, Guangyu1,2; Qiu, Tong1,2; Liu, Ying1,2 |
刊名 | TOXICON |
出版日期 | 2009-07-01 |
卷号 | 54期号:1页码:1-7 |
ISSN号 | 0041-0101 |
关键词 | Microcystin Fas/FasL Caspase Testis TUNEL staining Real-time PCR Western blot |
通讯作者 | Xie, P, Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China |
中文摘要 | Previous studies have shown that gonads were the second target organ of microcystins (MCs), and that MCs exposure exerted obvious toxic effects on male reproductive system of mammals. However, relevant molecular evidences are still lacking. Fas-signaling pathway plays a key role in toxicant-induced germ cell apoptosis. This study was to evaluate the responses of Fas/FasL system related genes and proteins in testes of rats injected intravenously with MCs. Enhanced apoptosis of germ cells in the testes of MCs-treated rats was detected by the terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick end labeling (TUNEL) associated with up-regulation of the Fas/FasL system. Both Fas and FasL protein expression were induced evidently from I h post-injection, and this high expression level maintained throughout the experiment. In addition, the activation of caspase-8 and caspase-3 protein was also observed, which were indicators of apoptosis. These results suggested the likely involvement of Fas/FasL system in the MCs-induced germ cell apoptosis. It is also suggested that MCs can cause damage to Sertoli cells directly. (C) 2009 Elsevier Ltd. All rights reserved. |
英文摘要 | Previous studies have shown that gonads were the second target organ of microcystins (MCs), and that MCs exposure exerted obvious toxic effects on male reproductive system of mammals. However, relevant molecular evidences are still lacking. Fas-signaling pathway plays a key role in toxicant-induced germ cell apoptosis. This study was to evaluate the responses of Fas/FasL system related genes and proteins in testes of rats injected intravenously with MCs. Enhanced apoptosis of germ cells in the testes of MCs-treated rats was detected by the terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick end labeling (TUNEL) associated with up-regulation of the Fas/FasL system. Both Fas and FasL protein expression were induced evidently from I h post-injection, and this high expression level maintained throughout the experiment. In addition, the activation of caspase-8 and caspase-3 protein was also observed, which were indicators of apoptosis. These results suggested the likely involvement of Fas/FasL system in the MCs-induced germ cell apoptosis. It is also suggested that MCs can cause damage to Sertoli cells directly. (C) 2009 Elsevier Ltd. All rights reserved. |
学科主题 | Pharmacology & Pharmacy; Toxicology |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Pharmacology & Pharmacy ; Toxicology |
研究领域[WOS] | Pharmacology & Pharmacy ; Toxicology |
关键词[WOS] | FAS LIGAND ; MONO-(2-ETHYLHEXYL) PHTHALATE ; TISSUE DISTRIBUTIONS ; REPRODUCTIVE-SYSTEM ; SEASONAL DYNAMICS ; SUBTROPICAL CHINA ; EUTROPHIC LAKE ; LARGE SHALLOW ; IN-VITRO ; LR |
资助信息 | National Basic Research Program of China [2008CB418101] |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000267011600001 |
公开日期 | 2010-10-13 |
源URL | [http://ir.ihb.ac.cn/handle/152342/7692] |
专题 | 水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文 |
作者单位 | 1.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100864, Peoples R China |
推荐引用方式 GB/T 7714 | Xiong, Qian,Xie, Ping,Li, Huiying,et al. Involvement of Fas/FasL system in apoptotic signaling in testicular germ cells of male Wistar rats injected i.v. with microcystins[J]. TOXICON,2009,54(1):1-7. |
APA | Xiong, Qian.,Xie, Ping.,Li, Huiying.,Hao, Le.,Li, Guangyu.,...&Liu, Ying.(2009).Involvement of Fas/FasL system in apoptotic signaling in testicular germ cells of male Wistar rats injected i.v. with microcystins.TOXICON,54(1),1-7. |
MLA | Xiong, Qian,et al."Involvement of Fas/FasL system in apoptotic signaling in testicular germ cells of male Wistar rats injected i.v. with microcystins".TOXICON 54.1(2009):1-7. |
入库方式: OAI收割
来源:水生生物研究所
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