Effects of adrenergic agents on the expression of zebrafish (Danio rerio) vitellogenin Ao1
文献类型：期刊论文
| 作者 | Yin, Naida1,2; Jin, Xia1; He, Jiangyan1; Yin, Zhan1,2 |
| 刊名 | TOXICOLOGY AND APPLIED PHARMACOLOGY
 |
| 出版日期 | 2009-07-01 |
| 卷号 | 238期号:1页码:20-26 |
| 关键词 | Zebrafish VtgAo1 Adrenergic agents Heart Liver |
| ISSN号 | 0041-008X |
| 通讯作者 | Yin, Z, Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Hubei, Peoples R China |
| 中文摘要 | Teleost vitellogenins (VTGs) are large multidomain apolipoproteins, traditionally considered to be estrogen-responsive precursors of the major egg yolk proteins, expressed and synthesized mainly in hepatic tissue. The inducibility of VTGs has made them one of the most frequently used in vivo and in vitro biomarkers of exposure to estrogen-active substances. A significant level of zebrafish vtgAo1, a major estrogen responsive form, has been unexpectedly found in heart tissue in our present studies. Our studies on zebrafish cardiomyopathy, caused by adrenergic agonist treatment, suggest a similar protective function of the cardiac expressed vtgAo1. We hypothesize that its function is to unload surplus intracellular lipids in cardiomyocytes for "reverse triglyceride transportation" similar to that found in lipid transport proteins in mammals. Our results also demonstrated that zebrafish vtgAo1 mRNA expression in heart can be suppressed by both (x-adrenergic agonist, phenylephrine (PE) and beta-adrenergic agonist, isoproterenol (ISO). Furthermore, the strong stimulation of zebrafish vtgAo1 expression in plasma induced by the beta-adrenergic antagonist, MOXIsylyl, was detected by Enzyme-Linked ImmunoSorbent Assay (ELISA). Such stimulation cannot be suppressed by taMOXIfen, an antagonist to estrogen receptors. Thus, Our present data indicate that the production of teleost VTG in vivo can be regulated not only by estrogenic agents, but by adrenergic signals as well. (c) 2009 Elsevier Inc. All rights reserved. |
| 英文摘要 | Teleost vitellogenins (VTGs) are large multidomain apolipoproteins, traditionally considered to be estrogen-responsive precursors of the major egg yolk proteins, expressed and synthesized mainly in hepatic tissue. The inducibility of VTGs has made them one of the most frequently used in vivo and in vitro biomarkers of exposure to estrogen-active substances. A significant level of zebrafish vtgAo1, a major estrogen responsive form, has been unexpectedly found in heart tissue in our present studies. Our studies on zebrafish cardiomyopathy, caused by adrenergic agonist treatment, suggest a similar protective function of the cardiac expressed vtgAo1. We hypothesize that its function is to unload surplus intracellular lipids in cardiomyocytes for "reverse triglyceride transportation" similar to that found in lipid transport proteins in mammals. Our results also demonstrated that zebrafish vtgAo1 mRNA expression in heart can be suppressed by both (x-adrenergic agonist, phenylephrine (PE) and beta-adrenergic agonist, isoproterenol (ISO). Furthermore, the strong stimulation of zebrafish vtgAo1 expression in plasma induced by the beta-adrenergic antagonist, MOXIsylyl, was detected by Enzyme-Linked ImmunoSorbent Assay (ELISA). Such stimulation cannot be suppressed by taMOXIfen, an antagonist to estrogen receptors. Thus, Our present data indicate that the production of teleost VTG in vivo can be regulated not only by estrogenic agents, but by adrenergic signals as well. (c) 2009 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Pharmacology & Pharmacy; Toxicology |
| 类目[WOS] | Pharmacology & Pharmacy ; Toxicology |
| 研究领域[WOS] | Pharmacology & Pharmacy ; Toxicology |
| 关键词[WOS] | MINNOW PIMEPHALES-PROMELAS ; APOLIPOPROTEIN-B ; EXTRAHEPATIC EXPRESSION ; MOLECULAR-CLONING ; GENE-EXPRESSION ; HEART ; TRIGLYCERIDE ; LIVER ; CHEMICALS ; CDNA |
| 收录类别 | SCI |
| 资助信息 | Hi-Tech Research and Development Program of China [2006AA06Z424]; National Natural Science Foundation of China [90608017, 20890113] |
| 语种 | 英语 |
| WOS记录号 | WOS:000267009800003 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7694]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yin, Naida,Jin, Xia,He, Jiangyan,et al. Effects of adrenergic agents on the expression of zebrafish (Danio rerio) vitellogenin Ao1[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY,2009,238(1):20-26. |
| APA | Yin, Naida,Jin, Xia,He, Jiangyan,&Yin, Zhan.(2009).Effects of adrenergic agents on the expression of zebrafish (Danio rerio) vitellogenin Ao1.TOXICOLOGY AND APPLIED PHARMACOLOGY,238(1),20-26. |
| MLA | Yin, Naida,et al."Effects of adrenergic agents on the expression of zebrafish (Danio rerio) vitellogenin Ao1".TOXICOLOGY AND APPLIED PHARMACOLOGY 238.1(2009):20-26. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。