Medaka vasa is required for migration but not survival of primordial germ cells
文献类型：期刊论文
| 作者 | Li, Mingyou ; Hong, Ni ; Xu, Hongyan ; Yi, Meisheng ; Li, Changming ; Gui, Jianfang ; Hong, Yunhan |
| 刊名 | MECHANISMS OF DEVELOPMENT
 |
| 出版日期 | 2009 |
| 卷号 | 126期号:5-6页码:366-381 |
| 关键词 | Cell migration Germ cell culture Morpholino Motility Oryzias latipes Primordial germ cell vasa |
| 通讯作者 | Hong, YH, Natl Univ Singapore, Dept Biol Sci, 10 Kent Ridge Crescent, Singapore 119260, Singapore |
| 中文摘要 | Vasa is essential for germline development. However, the precise processes in which vasa involves vary considerably in diverse animal phyla. Here we show that vasa is required for primordial germ cell (PGC) migration in the medakafish. vasa knockdown by two morpholinos led to the PGC migration defect that was rescued by coinjection of Vasa RNA. Interestingly, Vasa knockdown did not alter the PGC number, identity, proliferation and motility even at ectopic locations. We established a cell culture system for tracing PGCs at the single cell level in vitro. In this culture system, control and morpholino-injected gastrulae produced the same PGC number and the same time course of PGC survival. importantly, vasa-depleted PGCs in culture had similar motility and locomotion to normal PGCs. Expression patterns of wt1a, sdf1b and cxcT4b in migratory tissues remained unchanged by Vasa knockdown. By chimera formation we show that PGCs from vasa-depleted blastulae failed to migrate properly in the normal environment, whereas control PGCs migrated normally in vasa-disrupted embryos. Furthermore, ectopic PGCs in vasa-depleted embryos also retained all the PGC properties examined. Taken together, medaka vasa is cell-autonomously required for PGC migration, but dispensable to PGC proliferation, motility, identity and survival. (C) 2009 Elsevier Ireland Ltd. All rights reserved. |
| 学科主题 | Developmental Biology |
| 收录类别 | SCI |
| 语种 | 英语 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7700]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li, Mingyou,Hong, Ni,Xu, Hongyan,et al. Medaka vasa is required for migration but not survival of primordial germ cells[J]. MECHANISMS OF DEVELOPMENT,2009,126(5-6):366-381. |
| APA | Li, Mingyou.,Hong, Ni.,Xu, Hongyan.,Yi, Meisheng.,Li, Changming.,...&Hong, Yunhan.(2009).Medaka vasa is required for migration but not survival of primordial germ cells.MECHANISMS OF DEVELOPMENT,126(5-6),366-381. |
| MLA | Li, Mingyou,et al."Medaka vasa is required for migration but not survival of primordial germ cells".MECHANISMS OF DEVELOPMENT 126.5-6(2009):366-381. |
入库方式： OAI收割
来源：水生生物研究所
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