Identification of a novel gene K23 over-expressed in fish cross-subfamily cloned embryos
文献类型：期刊论文
| 作者 | Pei, De-Sheng1,2; Sun, Yong-Hua1; Zhu, Zuo-Yan1 |
| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2009-07-01 |
| 卷号 | 36期号:6页码:1375-1380 |
| 关键词 | Nuclear reprogramming Nuclear transfer Rare minnow (Gobiocypris rarus) Zebrafish (Danio rerio) |
| ISSN号 | 0301-4851 |
| 通讯作者 | Sun, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | A novel gene-K23, differentially expressed in cross-subfamily cloned embryos, was isolated by RACE-PCR technique. It had 2580 base pairs (bp) in length, with a 1,425 bp open reading frame (ORF) encoding a putative protein of 474 amino acids (aa). Bioinformatic analysis indicated that K23 had 22 phosphorylation sites, but it had no signal peptides. Developmental expression analysis in zebrafish showed that K23 transcripts were maternally expressed in ovum and the amount of K23 transcripts increased gradually from zygote to pharyngula period. Subcellular localization analysis revealed that K23 protein was homogeneously distributed both in nuclei and cytoplasm. Taken together, our findings indicate that K23 gene is a novel gene differentially expressed in fish cross-subfamily cloned embryos. |
| 英文摘要 | A novel gene-K23, differentially expressed in cross-subfamily cloned embryos, was isolated by RACE-PCR technique. It had 2580 base pairs (bp) in length, with a 1,425 bp open reading frame (ORF) encoding a putative protein of 474 amino acids (aa). Bioinformatic analysis indicated that K23 had 22 phosphorylation sites, but it had no signal peptides. Developmental expression analysis in zebrafish showed that K23 transcripts were maternally expressed in ovum and the amount of K23 transcripts increased gradually from zygote to pharyngula period. Subcellular localization analysis revealed that K23 protein was homogeneously distributed both in nuclei and cytoplasm. Taken together, our findings indicate that K23 gene is a novel gene differentially expressed in fish cross-subfamily cloned embryos. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | PLURIPOTENT STEM-CELLS ; NUCLEAR TRANSFER ; ENUCLEATED EGGS ; IN-VITRO ; ZEBRAFISH ; FIBROBLASTS ; GENERATION ; METHYLATION ; CLONING ; MOUSE |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [30700607, 30771100]; State Key Fundamental Research of China [2004CB117406] |
| 语种 | 英语 |
| WOS记录号 | WOS:000266454500023 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7712]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Grp Environm Genom, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pei, De-Sheng,Sun, Yong-Hua,Zhu, Zuo-Yan. Identification of a novel gene K23 over-expressed in fish cross-subfamily cloned embryos[J]. MOLECULAR BIOLOGY REPORTS,2009,36(6):1375-1380. |
| APA | Pei, De-Sheng,Sun, Yong-Hua,&Zhu, Zuo-Yan.(2009).Identification of a novel gene K23 over-expressed in fish cross-subfamily cloned embryos.MOLECULAR BIOLOGY REPORTS,36(6),1375-1380. |
| MLA | Pei, De-Sheng,et al."Identification of a novel gene K23 over-expressed in fish cross-subfamily cloned embryos".MOLECULAR BIOLOGY REPORTS 36.6(2009):1375-1380. |
入库方式： OAI收割
来源：水生生物研究所
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