Morphological and molecular phylogenetic analysis of two Saprolegnia sp (Oomycetes) isolated from silver crucian carp and zebra fish
文献类型：期刊论文
| 作者 | Ke, Xiao L.1,2; Wang, Jian G.1; Gu, Ze M.3; Li, Ming4; Gong, Xiao N.1 |
| 刊名 | MYCOLOGICAL RESEARCH
 |
| 出版日期 | 2009-05-01 |
| 卷号 | 113页码:637-644 |
| 关键词 | Fish disease ITS rDNA Molecular phylogeny Saprolegnia Systematic |
| ISSN号 | 0953-7562 |
| 通讯作者 | Wang, JG, Chinese Acad Sci, Inst Hydrobiol, Lab Healthy Aquaculture, Wuhan 430072, Peoples R China |
| 中文摘要 | Two Saprolegnia isolates, JY isolated from silver crucian carp (Carassius auratus gibelio Bloch) and BMY isolated from zebra fish (Brachydanio rerio Hamilton) came from infections occurring concurrently in different locations in China. To confirm whether the two isolates were from the same Saprolegnia clone, comparative studies have been carried out based on their morphological, physiological and molecular characteristics. Observations showed that morphologically (both asexual and sexual organs) the two isolates were broadly similar and both isolates under-went repeated zoospore emergence. Comparing 704 base pairs of internal transcribed spacer (ITS) region and the 5.8S rDNA, we found isolates JY and BMY shared an identical ITS sequence with a minor variation (99.6 % similarity). Forty available sequences for representatives Saprolegnia spp. belonged to four phylogenetically separate clades. The two studied isolates fell within clade I that comprised a group of isolates which showed almost an identical ITS sequence but had been identified as a number of different morphological species. our findings suggest that isolates JY and BMY appear to belong to the S. ferax clade and this clade (1) contains a number of closely related phylogenetic species. This is distinct from the more common fish pathogenic isolates, which belong to the S. parasitica clade (III) and are characterized by having cysts decorated by bundles of long hooked hairs and two further clades (II and IV) containing largely saprotrophic or soil born species. (C) 2009 Published by Elsevier Ltd on behalf of The British Mycological Society. |
| 英文摘要 | Two Saprolegnia isolates, JY isolated from silver crucian carp (Carassius auratus gibelio Bloch) and BMY isolated from zebra fish (Brachydanio rerio Hamilton) came from infections occurring concurrently in different locations in China. To confirm whether the two isolates were from the same Saprolegnia clone, comparative studies have been carried out based on their morphological, physiological and molecular characteristics. Observations showed that morphologically (both asexual and sexual organs) the two isolates were broadly similar and both isolates under-went repeated zoospore emergence. Comparing 704 base pairs of internal transcribed spacer (ITS) region and the 5.8S rDNA, we found isolates JY and BMY shared an identical ITS sequence with a minor variation (99.6 % similarity). Forty available sequences for representatives Saprolegnia spp. belonged to four phylogenetically separate clades. The two studied isolates fell within clade I that comprised a group of isolates which showed almost an identical ITS sequence but had been identified as a number of different morphological species. our findings suggest that isolates JY and BMY appear to belong to the S. ferax clade and this clade (1) contains a number of closely related phylogenetic species. This is distinct from the more common fish pathogenic isolates, which belong to the S. parasitica clade (III) and are characterized by having cysts decorated by bundles of long hooked hairs and two further clades (II and IV) containing largely saprotrophic or soil born species. (C) 2009 Published by Elsevier Ltd on behalf of The British Mycological Society. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Mycology |
| 类目[WOS] | Mycology |
| 研究领域[WOS] | Mycology |
| 关键词[WOS] | SEQUENCE ALIGNMENT ; BROWN TROUT ; PARASITICA ; FUNGUS ; AMPHIBIANS ; TAXONOMY ; DICLINA ; EGGS |
| 收录类别 | SCI |
| 资助信息 | Chinese Academy of Sciences [320321]; Hubei Province of China [2007AA203A01]; National 973 project [2009CB118700] |
| 语种 | 英语 |
| WOS记录号 | WOS:000266473900012 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7728]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Lab Healthy Aquaculture, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Huazhong Agr Univ, Coll Fisheries, Wuhan 430070, Peoples R China 4.Wuhan Polytech Univ, Feed Sci Dept, Wuhan 430023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ke, Xiao L.,Wang, Jian G.,Gu, Ze M.,et al. Morphological and molecular phylogenetic analysis of two Saprolegnia sp (Oomycetes) isolated from silver crucian carp and zebra fish[J]. MYCOLOGICAL RESEARCH,2009,113:637-644. |
| APA | Ke, Xiao L.,Wang, Jian G.,Gu, Ze M.,Li, Ming,&Gong, Xiao N..(2009).Morphological and molecular phylogenetic analysis of two Saprolegnia sp (Oomycetes) isolated from silver crucian carp and zebra fish.MYCOLOGICAL RESEARCH,113,637-644. |
| MLA | Ke, Xiao L.,et al."Morphological and molecular phylogenetic analysis of two Saprolegnia sp (Oomycetes) isolated from silver crucian carp and zebra fish".MYCOLOGICAL RESEARCH 113(2009):637-644. |
入库方式： OAI收割
来源：水生生物研究所
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