The Essential Role of Endogenous Ghrelin in Growth Hormone Expression during Zebrafish Adenohypophysis Development
文献类型：期刊论文
| 作者 | Li, Xi1,2; He, Jiangyan1; Hu, Wei1; Yin, Zhan1,2,3 |
| 刊名 | ENDOCRINOLOGY
 |
| 出版日期 | 2009-06-01 |
| 卷号 | 150期号:6页码:2767-2774 |
| 关键词 | DES-ACYL GHRELIN COMPLEMENTARY DEOXYRIBONUCLEIC-ACID FOOD-INTAKE SECRETAGOGUE RECEPTOR FUNCTIONAL-CHARACTERIZATION ENERGY-BALANCE GENE PITUITARY PEPTIDE OBESTATIN |
| ISSN号 | 0013-7227 |
| 通讯作者 | Yin, Z, Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Hubei, Peoples R China |
| 中文摘要 | Ghrelin, a multifunctional hormone, including potent GH stimulation activity, has been suggested to be important during embryonic development. Expression of ghrelin has been confirmed in the zebrafish pancreas during embryonic stages. Interfering with ghrelin function using two specific antisense morpholino oligonucleotides causes defects during zebrafish embryonic development. In ghrelin morphants the expression of GH was abolished in zebrafish somatotropes, whereas the expression patterns of the other key molecules involved in hypothalamic-pituitary development and distinct pituitary hormones genes remain largely intact at the appropriate time during zebrafish adenohypophysis development. Effective rescue of the ghrelin morphants with exogenous ghrelin mRNA showed that the correct gene had been targeted. Moreover, by analyzing the efficiencies of the ghrelin morphants rescue experiments with various forms of exogenous mutant ghrelin mRNAs, we also demonstrated the essentiality of the form acyl-ghrelin on GH stimulation during zebrafish adenohypophysis development. Our in vivo experiments, for the first time, also provided evidence of the existence of functional obestatin in the C-terminal part of zebrafish proghrelin peptides. Our research here has demonstrated that zebrafish is a unique model for functional studies of endogenous ghrelin, especially during embryonic development. (Endocrinology 150: 2767-2774, 2009) |
| 英文摘要 | Ghrelin, a multifunctional hormone, including potent GH stimulation activity, has been suggested to be important during embryonic development. Expression of ghrelin has been confirmed in the zebrafish pancreas during embryonic stages. Interfering with ghrelin function using two specific antisense morpholino oligonucleotides causes defects during zebrafish embryonic development. In ghrelin morphants the expression of GH was abolished in zebrafish somatotropes, whereas the expression patterns of the other key molecules involved in hypothalamic-pituitary development and distinct pituitary hormones genes remain largely intact at the appropriate time during zebrafish adenohypophysis development. Effective rescue of the ghrelin morphants with exogenous ghrelin mRNA showed that the correct gene had been targeted. Moreover, by analyzing the efficiencies of the ghrelin morphants rescue experiments with various forms of exogenous mutant ghrelin mRNAs, we also demonstrated the essentiality of the form acyl-ghrelin on GH stimulation during zebrafish adenohypophysis development. Our in vivo experiments, for the first time, also provided evidence of the existence of functional obestatin in the C-terminal part of zebrafish proghrelin peptides. Our research here has demonstrated that zebrafish is a unique model for functional studies of endogenous ghrelin, especially during embryonic development. (Endocrinology 150: 2767-2774, 2009) |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Endocrinology & Metabolism |
| 类目[WOS] | Endocrinology & Metabolism |
| 研究领域[WOS] | Endocrinology & Metabolism |
| 关键词[WOS] | DES-ACYL GHRELIN ; COMPLEMENTARY DEOXYRIBONUCLEIC-ACID ; FOOD-INTAKE ; SECRETAGOGUE RECEPTOR ; FUNCTIONAL-CHARACTERIZATION ; ENERGY-BALANCE ; GENE ; PITUITARY ; PEPTIDE ; OBESTATIN |
| 收录类别 | SCI |
| 资助信息 | National Basic Research Program of China (973 Program) [2004CB117402]; National Natural Science Foundation, China [20890113] |
| 语种 | 英语 |
| WOS记录号 | WOS:000266256700038 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7736]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Key Lab Biodivers & Conservat Aquat Organisms, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China 3.Huazhong Univ Sci & Technol, Chinese Minist Educ, Key Lab Mol Biophys, Wuhan 430074, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Xi,He, Jiangyan,Hu, Wei,et al. The Essential Role of Endogenous Ghrelin in Growth Hormone Expression during Zebrafish Adenohypophysis Development[J]. ENDOCRINOLOGY,2009,150(6):2767-2774. |
| APA | Li, Xi,He, Jiangyan,Hu, Wei,&Yin, Zhan.(2009).The Essential Role of Endogenous Ghrelin in Growth Hormone Expression during Zebrafish Adenohypophysis Development.ENDOCRINOLOGY,150(6),2767-2774. |
| MLA | Li, Xi,et al."The Essential Role of Endogenous Ghrelin in Growth Hormone Expression during Zebrafish Adenohypophysis Development".ENDOCRINOLOGY 150.6(2009):2767-2774. |
入库方式： OAI收割
来源：水生生物研究所
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