Molecular characterization of Chinese sturgeon gonadotropins and cellular distribution in pituitaries of mature and immature individuals
文献类型：期刊论文
| 作者 | Cao, Hong1; Zhou, Li1; Zhang, Yan-Zhen2; Wei, Qi-Wei2; Chen, Xi-Hua2; Gui, Jian-Fang1 |
| 刊名 | MOLECULAR AND CELLULAR ENDOCRINOLOGY
 |
| 出版日期 | 2009-05-06 |
| 卷号 | 303期号:1-2页码:34-42 |
| 关键词 | Gonadotropin Acipenser sinensis Pituitary AsGTH-alpha I AsFSH beta AsLH beta |
| ISSN号 | 0303-7207 |
| 通讯作者 | Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Chinese sturgeon (Acipenser sinensis) is a rare and endangered species, and also an important resource for the sturgeon aquaculture industry. To understand molecular characterization of Chinese sturgeon gonadotropins (GTHs), we cloned the full-length cDNAs of gonadotropin subunits common alpha (GTH-alpha), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) from a pituitary cDNA library of mature female. Two subtypes of GTH-alpha were identified. The nucleotide sequences of A. sinensis common alpha I (AsGTH-alpha I), common alpha II (AsGTH-alpha II), FSH beta (AsFSH beta) and LH beta (AsLH beta) subunit cDNAs are 345, 363, 387 and 414 bp in length, and encode mature peptides of 115, 121, 129 and 138 aa, respectively. Then, three polyclonal antibodies were prepared from the in vitro expressed AsGTH-alpha I, AsFSH beta and AsLH beta mature proteins, respectively. Significant expression differences were revealed between immature and mature sturgeon pituitaries. Western blot detection and immunofluoresence localization revealed the existence of three-gonadotropin subunits (AsGTH-alpha, AsFSH beta and AsLH beta) in mature sturgeon pituitaries, but only AsFSH beta was detected in immature individual pituitaries during early stages in the sturgeon life, and obvious difference was observed between males and females. In males, AsFSH beta was expressed in 4-year-old individuals, whereas in females, AsFSH beta was just expressed in 5-year-old individuals. (C) 2009 Elsevier Ireland Ltd. All rights reserved. |
| 英文摘要 | Chinese sturgeon (Acipenser sinensis) is a rare and endangered species, and also an important resource for the sturgeon aquaculture industry. To understand molecular characterization of Chinese sturgeon gonadotropins (GTHs), we cloned the full-length cDNAs of gonadotropin subunits common alpha (GTH-alpha), follicle-stimulating hormone (FSH) and luteinizing hormone (LH) from a pituitary cDNA library of mature female. Two subtypes of GTH-alpha were identified. The nucleotide sequences of A. sinensis common alpha I (AsGTH-alpha I), common alpha II (AsGTH-alpha II), FSH beta (AsFSH beta) and LH beta (AsLH beta) subunit cDNAs are 345, 363, 387 and 414 bp in length, and encode mature peptides of 115, 121, 129 and 138 aa, respectively. Then, three polyclonal antibodies were prepared from the in vitro expressed AsGTH-alpha I, AsFSH beta and AsLH beta mature proteins, respectively. Significant expression differences were revealed between immature and mature sturgeon pituitaries. Western blot detection and immunofluoresence localization revealed the existence of three-gonadotropin subunits (AsGTH-alpha, AsFSH beta and AsLH beta) in mature sturgeon pituitaries, but only AsFSH beta was detected in immature individual pituitaries during early stages in the sturgeon life, and obvious difference was observed between males and females. In males, AsFSH beta was expressed in 4-year-old individuals, whereas in females, AsFSH beta was just expressed in 5-year-old individuals. (C) 2009 Elsevier Ireland Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Cell Biology; Endocrinology & Metabolism |
| 类目[WOS] | Cell Biology ; Endocrinology & Metabolism |
| 研究领域[WOS] | Cell Biology ; Endocrinology & Metabolism |
| 关键词[WOS] | FOLLICLE-STIMULATING-HORMONE ; 2 DISTINCT GONADOTROPINS ; GTH-II ; REPRODUCTIVE-CYCLE ; RAINBOW-TROUT ; FSH-BETA ; LH-BETA ; ACIPENSER-TRANSMONTANUS ; GLYCOPROTEIN HORMONE ; LUTEINIZING-HORMONE |
| 收录类别 | SCI |
| 资助信息 | National Major Basic Research Program [2004CB117401]; Chinese Academy of Sciences [KSCX2-YW-N-020]; State Key Laboratory of Freshwater Ecology and Biotechnology [2008FB007] |
| 语种 | 英语 |
| WOS记录号 | WOS:000265521200005 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7762]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Agr Minist China, Key Lab freshwater Fish Germplasm Resources & Bio, Jinzhou 434000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cao, Hong,Zhou, Li,Zhang, Yan-Zhen,et al. Molecular characterization of Chinese sturgeon gonadotropins and cellular distribution in pituitaries of mature and immature individuals[J]. MOLECULAR AND CELLULAR ENDOCRINOLOGY,2009,303(1-2):34-42. |
| APA | Cao, Hong,Zhou, Li,Zhang, Yan-Zhen,Wei, Qi-Wei,Chen, Xi-Hua,&Gui, Jian-Fang.(2009).Molecular characterization of Chinese sturgeon gonadotropins and cellular distribution in pituitaries of mature and immature individuals.MOLECULAR AND CELLULAR ENDOCRINOLOGY,303(1-2),34-42. |
| MLA | Cao, Hong,et al."Molecular characterization of Chinese sturgeon gonadotropins and cellular distribution in pituitaries of mature and immature individuals".MOLECULAR AND CELLULAR ENDOCRINOLOGY 303.1-2(2009):34-42. |
入库方式： OAI收割
来源：水生生物研究所
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