Isolation of a novel cyanophage infectious to the filamentous cyanobacterium Planktothrix agardhii (Cyanophyceae) from Lake Donghu, China
文献类型：期刊论文
| 作者 | Gao, E-Bin; Yuan, Xiu-Ping; Li, Ren-Hui; Zhang, Qi-Ya |
| 刊名 | AQUATIC MICROBIAL ECOLOGY
 |
| 出版日期 | 2009 |
| 卷号 | 54期号:2页码:163-170 |
| 关键词 | Cyanophage Planktothrix agardhii Viral infection Blooms Cyanobacteria Freshwater lake |
| ISSN号 | 0948-3055 |
| 通讯作者 | Zhang, QY, Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | A previously unknown cyanophage, PaV-LD (Planktothrix agardhii Virus isolated from Lake Donghu), which causes lysis of the bloom-forming filamentous cyanobacterium P. agardhii, was isolated from Lake Donghu, Wuhan, China. PaV-LD only lysed P. agardhii strains isolated from Lake Donghu and not those isolated from other lakes. The PaV-LD particle has an icosahedral, non-tailed structure, ca. 70 to 85 nm (mean +/- SD = 76 +/- 6 nm) in diameter. PaV-LD was stable at freezing temperature, but lost its infectivity at temperatures >50 degrees C. Lysis of host cells was delayed about 3 d after the PaV-LD treatment with chloroform, and the virus was inactivated by exposure to low pH (<= 4). The latent period and burst size of the PaV-LD were estimated to be 48 to 72 h and about 340 infectious units per cell, respectively. The regrowth cultures of surviving host filaments were not lysed by the PaV-LD suspension. To our knowledge, this is the first isolation and cultivation of a virus infectious to the filamentous bloom-forming cyanobacterium Planktothrix from a freshwater lake. |
| 英文摘要 | A previously unknown cyanophage, PaV-LD (Planktothrix agardhii Virus isolated from Lake Donghu), which causes lysis of the bloom-forming filamentous cyanobacterium P. agardhii, was isolated from Lake Donghu, Wuhan, China. PaV-LD only lysed P. agardhii strains isolated from Lake Donghu and not those isolated from other lakes. The PaV-LD particle has an icosahedral, non-tailed structure, ca. 70 to 85 nm (mean +/- SD = 76 +/- 6 nm) in diameter. PaV-LD was stable at freezing temperature, but lost its infectivity at temperatures >50 degrees C. Lysis of host cells was delayed about 3 d after the PaV-LD treatment with chloroform, and the virus was inactivated by exposure to low pH (<= 4). The latent period and burst size of the PaV-LD were estimated to be 48 to 72 h and about 340 infectious units per cell, respectively. The regrowth cultures of surviving host filaments were not lysed by the PaV-LD suspension. To our knowledge, this is the first isolation and cultivation of a virus infectious to the filamentous bloom-forming cyanobacterium Planktothrix from a freshwater lake. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Ecology; Marine & Freshwater Biology; Microbiology |
| 类目[WOS] | Ecology ; Marine & Freshwater Biology ; Microbiology |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Marine & Freshwater Biology ; Microbiology |
| 关键词[WOS] | MICROCYSTIS-AERUGINOSA ; MARINE SYNECHOCOCCUS ; BLOOM ; VIRUSES ; DIVERSITY ; WATER ; VIRIOPLANKTON ; COMMUNITIES ; DYNAMICS ; SHALLOW |
| 收录类别 | SCI |
| 资助信息 | National Major Basic Research Program [2004CB117403]; National 863 High Technology Research Foundation of China ; National Natural Science Foundation of China [30671616, U0631008] |
| 语种 | 英语 |
| WOS记录号 | WOS:000265158200005 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7786]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Grad Univ, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, E-Bin,Yuan, Xiu-Ping,Li, Ren-Hui,et al. Isolation of a novel cyanophage infectious to the filamentous cyanobacterium Planktothrix agardhii (Cyanophyceae) from Lake Donghu, China[J]. AQUATIC MICROBIAL ECOLOGY,2009,54(2):163-170. |
| APA | Gao, E-Bin,Yuan, Xiu-Ping,Li, Ren-Hui,&Zhang, Qi-Ya.(2009).Isolation of a novel cyanophage infectious to the filamentous cyanobacterium Planktothrix agardhii (Cyanophyceae) from Lake Donghu, China.AQUATIC MICROBIAL ECOLOGY,54(2),163-170. |
| MLA | Gao, E-Bin,et al."Isolation of a novel cyanophage infectious to the filamentous cyanobacterium Planktothrix agardhii (Cyanophyceae) from Lake Donghu, China".AQUATIC MICROBIAL ECOLOGY 54.2(2009):163-170. |
入库方式： OAI收割
来源：水生生物研究所
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