Effects of pure microcystin-LR on the transcription of immune related genes and heat shock proteins in larval stage of zebrafish (Danio rerio)
文献类型：期刊论文
| 作者 | Li, Yuan1,2; Sun, Baojian1; Wu, Hongjuan2; Nie, Pin1 |
| 刊名 | AQUACULTURE
 |
| 出版日期 | 2009-04-03 |
| 卷号 | 289期号:1-2页码:154-160 |
| 关键词 | Microcystin Immunotoxin T cell development Heat shock proteins Gene expression Zebrafish |
| ISSN号 | 0044-8486 |
| 通讯作者 | Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China |
| 中文摘要 | Microcystins (MCs) are cyanobacterial toxins in water blooms that have received increasing attention as a public biohazard for human and animal health. Previous studies were mainly focused on the toxic effects on adult fish, rather than juvenile or larvae, and the response of fish immune system were usually neglected. This paper presents the first data of the effects of microcystin-LR (MC-LR) on transcription of several genes essential for early lymphoid development (Rag1, Rag2, Ikaros, GATA1, Lck and TCR alpha) and heat shock proteins (HSP90, HSP70, HSP60, HSP27) in zebrafish larvae. Relative changes of mRNA transcription were analyzed by real time PCR. The transcription of Rag1, Rag2, Ikaros, GATA1, Lck and TCR alpha were up-regulated when following exposure to 800 mu g/L MC-LR, which may indicate that specific lymphocytes differentiation and TCR/lg arrangement are induced to counteract the toxic effects of MC-LR. It was also interesting to note the dramatically increased transcription of HSP90. HSP70, HSP60 and HSP27, which may indicate their important roles as molecular chaperones under oxidative stress. (C) 2009 Elsevier B.V. All rights reserved. |
| 英文摘要 | Microcystins (MCs) are cyanobacterial toxins in water blooms that have received increasing attention as a public biohazard for human and animal health. Previous studies were mainly focused on the toxic effects on adult fish, rather than juvenile or larvae, and the response of fish immune system were usually neglected. This paper presents the first data of the effects of microcystin-LR (MC-LR) on transcription of several genes essential for early lymphoid development (Rag1, Rag2, Ikaros, GATA1, Lck and TCR alpha) and heat shock proteins (HSP90, HSP70, HSP60, HSP27) in zebrafish larvae. Relative changes of mRNA transcription were analyzed by real time PCR. The transcription of Rag1, Rag2, Ikaros, GATA1, Lck and TCR alpha were up-regulated when following exposure to 800 mu g/L MC-LR, which may indicate that specific lymphocytes differentiation and TCR/lg arrangement are induced to counteract the toxic effects of MC-LR. It was also interesting to note the dramatically increased transcription of HSP90. HSP70, HSP60 and HSP27, which may indicate their important roles as molecular chaperones under oxidative stress. (C) 2009 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries; Marine & Freshwater Biology |
| 类目[WOS] | Fisheries ; Marine & Freshwater Biology |
| 研究领域[WOS] | Fisheries ; Marine & Freshwater Biology |
| 关键词[WOS] | OXIDATIVE STRESS ; HEPATIC HEAT-SHOCK-PROTEIN-70 ; CYANOBACTERIAL TOXINS ; BETA-NAPHTHOFLAVONE ; MOUSE-LIVER ; DNA-DAMAGE ; IN-VIVO ; EXPRESSION ; APOPTOSIS ; FISH |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [20577065, 20747003] |
| 语种 | 英语 |
| WOS记录号 | WOS:000264942400025 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7794]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei Province, Peoples R China 2.Huazhong Univ Sci & Technol, Coll Environm Sci & Engn, Wuhan 430074, Hubei Province, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yuan,Sun, Baojian,Wu, Hongjuan,et al. Effects of pure microcystin-LR on the transcription of immune related genes and heat shock proteins in larval stage of zebrafish (Danio rerio)[J]. AQUACULTURE,2009,289(1-2):154-160. |
| APA | Li, Yuan,Sun, Baojian,Wu, Hongjuan,&Nie, Pin.(2009).Effects of pure microcystin-LR on the transcription of immune related genes and heat shock proteins in larval stage of zebrafish (Danio rerio).AQUACULTURE,289(1-2),154-160. |
| MLA | Li, Yuan,et al."Effects of pure microcystin-LR on the transcription of immune related genes and heat shock proteins in larval stage of zebrafish (Danio rerio)".AQUACULTURE 289.1-2(2009):154-160. |
入库方式： OAI收割
来源：水生生物研究所
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