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Identification of Differentially Expressed Genes Between Cloned and Zygote-Developing Zebrafish (Danio rerio) Embryos at the Dome Stage Using Suppression Subtractive Hybridization

文献类型：期刊论文

作者Luo, Daji1,2; Hu, Wei2; Chen, Shangping2; Xiao, Yi1; Sun, Yonghua2; Zhu, Zuoyan1,2
刊名BIOLOGY OF REPRODUCTION
出版日期2009-04-01
卷号80期号:4页码:674-684
关键词embryo differentially expressed genes reprogramming somatic cell nuclear transfer suppression subtractive hybridization
ISSN号0006-3363
通讯作者Zhu, ZY, Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
中文摘要Comparative analyses of differentially expressed genes between somatic cell nuclear transfer (SCNT) embryos and zygote-developing (ZD) embryos are important for understanding the molecular mechanism underlying the reprogramming processes. Herein, we used the suppression subtractive hybridization approach and from more than 2900 clones identified 96 differentially expressed genes between the SCNT and ZD embryos at the dome stage in zebrafish. We report the first database of differentially expressed genes in zebrafish SCNT embryos. Collectively, our findings demonstrate that zebrafish SCNT embryos undergo significant reprogramming processes during the dome stage. However, most differentially expressed genes are down-regulated in SCNT embryos, indicating failure of reprogramming. Based on Ensembl description and Gene Ontology Consortium annotation, the problems of reprogramming at the dome stage may occur during nuclear remodeling, translation initiation, and regulation of the cell cycle. The importance of regulation from recipient oocytes in cloning should not be underestimated in zebrafish.
英文摘要Comparative analyses of differentially expressed genes between somatic cell nuclear transfer (SCNT) embryos and zygote-developing (ZD) embryos are important for understanding the molecular mechanism underlying the reprogramming processes. Herein, we used the suppression subtractive hybridization approach and from more than 2900 clones identified 96 differentially expressed genes between the SCNT and ZD embryos at the dome stage in zebrafish. We report the first database of differentially expressed genes in zebrafish SCNT embryos. Collectively, our findings demonstrate that zebrafish SCNT embryos undergo significant reprogramming processes during the dome stage. However, most differentially expressed genes are down-regulated in SCNT embryos, indicating failure of reprogramming. Based on Ensembl description and Gene Ontology Consortium annotation, the problems of reprogramming at the dome stage may occur during nuclear remodeling, translation initiation, and regulation of the cell cycle. The importance of regulation from recipient oocytes in cloning should not be underestimated in zebrafish.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
学科主题Reproductive Biology
类目[WOS]Reproductive Biology
研究领域[WOS]Reproductive Biology
关键词[WOS]SOMATIC-CELL NUCLEI ; PLURIPOTENT STEM-CELLS ; MEDAKA ORYZIAS-LATIPES ; TUMOR-SUPPRESSOR ; ENUCLEATED EGGS ; FISH ; P53 ; CYTOPLASM ; OOCYTES ; CLONING
收录类别SCI
资助信息Development Plan of the State Key Fundamental Research of China [2007CB109206]; National Natural Science Foundation [30430540]
语种英语
WOS记录号WOS:000264437000009
公开日期2010-10-13
源URL[http://ir.ihb.ac.cn/handle/152342/7826]  
专题水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文
作者单位1.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
推荐引用方式
GB/T 7714		 Luo, Daji,Hu, Wei,Chen, Shangping,et al. Identification of Differentially Expressed Genes Between Cloned and Zygote-Developing Zebrafish (Danio rerio) Embryos at the Dome Stage Using Suppression Subtractive Hybridization[J]. BIOLOGY OF REPRODUCTION,2009,80(4):674-684.
APA Luo, Daji,Hu, Wei,Chen, Shangping,Xiao, Yi,Sun, Yonghua,&Zhu, Zuoyan.(2009).Identification of Differentially Expressed Genes Between Cloned and Zygote-Developing Zebrafish (Danio rerio) Embryos at the Dome Stage Using Suppression Subtractive Hybridization.BIOLOGY OF REPRODUCTION,80(4),674-684.
MLA Luo, Daji,et al."Identification of Differentially Expressed Genes Between Cloned and Zygote-Developing Zebrafish (Danio rerio) Embryos at the Dome Stage Using Suppression Subtractive Hybridization".BIOLOGY OF REPRODUCTION 80.4(2009):674-684.

入库方式： OAI收割

来源：水生生物研究所

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