Rana grylio virus thymidine kinase gene: an early gene of iridovirus encoding for a cytoplasmic protein
文献类型：期刊论文
| 作者 | Zhao, Zhe; Ke, Fei; Shi, Yan; Zhou, Guang-; Gui, Jian-Fang; Zhang, Qi-Ya |
| 刊名 | VIRUS GENES
 |
| 出版日期 | 2009-04-01 |
| 卷号 | 38期号:2页码:345-352 |
| ISSN号 | 0920-8569 |
| 关键词 | Rana grylio virus (RGV) Iridovirus Thymidine kinase Early viral gene Cytoplasmic protein |
| 通讯作者 | Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | The presence of thymidine kinase (TK) is a feature of many large DNA viruses. Here, a TK gene homologue was cloned and characterized from Rana grylio virus (RGV), a member of family Iridoviridae. RGV TK encodes a protein of 195 aa with a predicted molecular mass of 22.1 kDa. Homologues of the protein were present in all the currently sequenced iridoviruses, and phylogenetic analysis showed that it was much close to cellular TK type 2 (TK2), deoxycytidine kinase (dCK) and deoxyguanosine kinase (dGK). Subsequently, Western blotting revealed TK expression increased with time from 6 h post-infection in RGV-infected cells. Using drug inhibition analysis by protein synthesis inhibitor (cycloheximide) and DNA replication inhibitor (cytosine arabinofuranoside), RGV TK was classified as the early expression gene during in vitro infection. Subcellular localization by TK-GFP fusion protein expression and immunofluorescence staining showed RGV TK was an exclusively cytoplasmic protein in fish cells. Collectively, current data indicate that RGV TK was an early gene of iridovirus which encoded a cytoplasmic protein in fish cells. |
| 英文摘要 | The presence of thymidine kinase (TK) is a feature of many large DNA viruses. Here, a TK gene homologue was cloned and characterized from Rana grylio virus (RGV), a member of family Iridoviridae. RGV TK encodes a protein of 195 aa with a predicted molecular mass of 22.1 kDa. Homologues of the protein were present in all the currently sequenced iridoviruses, and phylogenetic analysis showed that it was much close to cellular TK type 2 (TK2), deoxycytidine kinase (dCK) and deoxyguanosine kinase (dGK). Subsequently, Western blotting revealed TK expression increased with time from 6 h post-infection in RGV-infected cells. Using drug inhibition analysis by protein synthesis inhibitor (cycloheximide) and DNA replication inhibitor (cytosine arabinofuranoside), RGV TK was classified as the early expression gene during in vitro infection. Subcellular localization by TK-GFP fusion protein expression and immunofluorescence staining showed RGV TK was an exclusively cytoplasmic protein in fish cells. Collectively, current data indicate that RGV TK was an early gene of iridovirus which encoded a cytoplasmic protein in fish cells. |
| 学科主题 | Genetics & Heredity; Virology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Genetics & Heredity ; Virology |
| 研究领域[WOS] | Genetics & Heredity ; Virology |
| 关键词[WOS] | HERPES-SIMPLEX-VIRUS ; LYMPHOCYSTIS DISEASE VIRUS ; COMPLETE GENOME SEQUENCE ; CHILO IRIDESCENT VIRUS ; RANAVIRUS FAMILY IRIDOVIRIDAE ; SINGAPORE GROUPER IRIDOVIRUS ; COMPLETE DNA-SEQUENCE ; MAJOR CAPSID PROTEIN ; SPOT SYNDROME VIRUS ; DEOXYRIBONUCLEOSIDE KINASES |
| 资助信息 | National Major Basic Research Program [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Key Technology R & D Program of China [2006BAD03B05] |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000263900500020 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7844]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, Grad Sch, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Zhe,Ke, Fei,Shi, Yan,et al. Rana grylio virus thymidine kinase gene: an early gene of iridovirus encoding for a cytoplasmic protein[J]. VIRUS GENES,2009,38(2):345-352. |
| APA | Zhao, Zhe,Ke, Fei,Shi, Yan,Zhou, Guang-,Gui, Jian-Fang,&Zhang, Qi-Ya.(2009).Rana grylio virus thymidine kinase gene: an early gene of iridovirus encoding for a cytoplasmic protein.VIRUS GENES,38(2),345-352. |
| MLA | Zhao, Zhe,et al."Rana grylio virus thymidine kinase gene: an early gene of iridovirus encoding for a cytoplasmic protein".VIRUS GENES 38.2(2009):345-352. |
入库方式： OAI收割
来源：水生生物研究所
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