Depletion of Vipp1 in Synechocystis sp PCC 6803 affects photosynthetic activity before the loss of thylakoid membranes
文献类型：期刊论文
| 作者 | Gao, Hong; Xu, Xudong |
| 刊名 | FEMS MICROBIOLOGY LETTERS
 |
| 出版日期 | 2009-03-01 |
| 卷号 | 292期号:1页码:63-70 |
| 关键词 | Vipp1 P(petE) photosynthesis thylakoid membranes viability Synechocystis |
| ISSN号 | 0378-1097 |
| 通讯作者 | Xu, XD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China |
| 中文摘要 | A vipp1 mutant of Synechocystis sp. PCC 6803 could not be completely segregated under either mixotrophic or heterotrophic conditions. A vipp1 gene with a copper-regulated promoter (P-petE-vipp1) was integrated into a neutral platform in the genome of the merodiploid mutant. The copper-induced expression of P-petE-vipp1 allowed a complete segregation of the vipp1 mutant and observation of the phenotype of Synechocystis 6803 with different levels of vesicle-inducing protein in plastids 1 (Vipp1). When P-petE-vipp1 was turned off by copper deprivation, Synechocystis lost Vipp1 and photosynthetic activity almost simultaneously, and at a later stage, thylakoid membranes and cell viability. The photosystem II (PSII)-mediated electron transfer was much more rapidly reduced than the PSI-mediated electron transfer. By testing a series of concentrations, we found that P-petE-vipp1 cells grown in medium with 0.025 mu M Cu2+ showed no reduction of thylakoid membranes, but greatly reduced photosynthetic activity and viability. These results suggested that in contrast to a previous report, the loss of photosynthetic activity may not have been due to the loss of thylakoid membranes, but may have been caused more directly by the loss of Vipp1 in Synechocystis 6803. |
| 英文摘要 | A vipp1 mutant of Synechocystis sp. PCC 6803 could not be completely segregated under either mixotrophic or heterotrophic conditions. A vipp1 gene with a copper-regulated promoter (P(petE)-vipp1) was integrated into a neutral platform in the genome of the merodiploid mutant. The copper-induced expression of P(petE)-vipp1 allowed a complete segregation of the vipp1 mutant and observation of the phenotype of Synechocystis 6803 with different levels of vesicle-inducing protein in plastids 1 (Vipp1). When P(petE)-vipp1 was turned off by copper deprivation, Synechocystis lost Vipp1 and photosynthetic activity almost simultaneously, and at a later stage, thylakoid membranes and cell viability. The photosystem II (PSII)-mediated electron transfer was much more rapidly reduced than the PSI-mediated electron transfer. By testing a series of concentrations, we found that P(petE)-vipp1 cells grown in medium with 0.025 mu M Cu(2+) showed no reduction of thylakoid membranes, but greatly reduced photosynthetic activity and viability. These results suggested that in contrast to a previous report, the loss of photosynthetic activity may not have been due to the loss of thylakoid membranes, but may have been caused more directly by the loss of Vipp1 in Synechocystis 6803. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Microbiology |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | SP STRAIN PCC-6803 ; CHILL-LIGHT TOLERANCE ; PHOTOSYSTEM-II ; MOLECULAR-CLONING ; GENE ; BIOGENESIS ; EXPRESSION ; PLASMA ; GROWTH ; MUTANT |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [30330030]; Chinese Academy of Sciences [KSCX2-SW-332] |
| 语种 | 英语 |
| WOS记录号 | WOS:000263036900010 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7876]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gao, Hong,Xu, Xudong. Depletion of Vipp1 in Synechocystis sp PCC 6803 affects photosynthetic activity before the loss of thylakoid membranes[J]. FEMS MICROBIOLOGY LETTERS,2009,292(1):63-70. |
| APA | Gao, Hong,&Xu, Xudong.(2009).Depletion of Vipp1 in Synechocystis sp PCC 6803 affects photosynthetic activity before the loss of thylakoid membranes.FEMS MICROBIOLOGY LETTERS,292(1),63-70. |
| MLA | Gao, Hong,et al."Depletion of Vipp1 in Synechocystis sp PCC 6803 affects photosynthetic activity before the loss of thylakoid membranes".FEMS MICROBIOLOGY LETTERS 292.1(2009):63-70. |
入库方式： OAI收割
来源：水生生物研究所
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