Effects of adrenergic agonists on the extrahepatic expression of vitellogenin Ao1 in heart and brain of the Chinese rare minnow (Gobiocypris rarus)
文献类型：期刊论文
| 作者 | Ma, Liwen1,2; Li, Dongliang1,2; Wang, Jianwei1; He, Jiangyan1; Yin, Zhan1,2 |
| 刊名 | AQUATIC TOXICOLOGY
 |
| 出版日期 | 2009-01-18 |
| 卷号 | 91期号:1页码:19-25 |
| 关键词 | Chinese rare minnow vtgAo1 Adrenergic agonist Heart Liver |
| ISSN号 | 0166-445X |
| 通讯作者 | Yin, Z, Chinese Acad Sci, Inst Hydrobiol, 7 Donghu S St, Wuhan 430072, Peoples R China |
| 中文摘要 | Teleost vitellogenins (VTGs) are large multidomain apolipoproteins and traditionally considered as the estrogen responsive precursors of the major egg yolk proteins. We identified five clones encoding VTGs, about 16% of the random EST clones from our constructed cDNA library from Chinese rare minnow liver tissue treated with 17 beta-estradiol (E2). Full-length vtgAo1 has been obtained based on the sequence information of four partial cDNA inserts by RACE. The inducibility of the vtgAo1 expression in liver by E2 was confirmed by RT-PCR. The presence of vtgAo1 transcripts have been observed primarily in liver. However. a significant level of the vtgAo1 was found in an unexpected location, heart, particularly in atrial cells by RT-PCR and whole mount in situ hybridization analyses. The vtgAo1 mRNA expression in heart and liver tissue could be suppressed by both alpha-adrenergic agonist, phenylephrine (PE) and beta-adrenergic agonist, isoproterenol (ISO). The expression of VTG in the heart observed in the present studies suggested it may provide protection from surplus intracellular lipids in fish cardiomyocytes as triglyceride transport proteins do in mammals. The results also indicated that the production of teleost vtg in vivo can be regulated by riot only estrogenic agents, but adrenergic signals as well. (c) 2008 Elsevier B.V. All rights reserved. |
| 英文摘要 | Teleost vitellogenins (VTGs) are large multidomain apolipoproteins and traditionally considered as the estrogen responsive precursors of the major egg yolk proteins. We identified five clones encoding VTGs, about 16% of the random EST clones from our constructed cDNA library from Chinese rare minnow liver tissue treated with 17 beta-estradiol (E2). Full-length vtgAo1 has been obtained based on the sequence information of four partial cDNA inserts by RACE. The inducibility of the vtgAo1 expression in liver by E2 was confirmed by RT-PCR. The presence of vtgAo1 transcripts have been observed primarily in liver. However. a significant level of the vtgAo1 was found in an unexpected location, heart, particularly in atrial cells by RT-PCR and whole mount in situ hybridization analyses. The vtgAo1 mRNA expression in heart and liver tissue could be suppressed by both alpha-adrenergic agonist, phenylephrine (PE) and beta-adrenergic agonist, isoproterenol (ISO). The expression of VTG in the heart observed in the present studies suggested it may provide protection from surplus intracellular lipids in fish cardiomyocytes as triglyceride transport proteins do in mammals. The results also indicated that the production of teleost vtg in vivo can be regulated by riot only estrogenic agents, but adrenergic signals as well. (c) 2008 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Marine & Freshwater Biology; Toxicology |
| 类目[WOS] | Marine & Freshwater Biology ; Toxicology |
| 研究领域[WOS] | Marine & Freshwater Biology ; Toxicology |
| 关键词[WOS] | ZEBRAFISH DANIO-RERIO ; APOLIPOPROTEIN-B ; ENDOCRINE DISRUPTION ; PIMEPHALES-PROMELAS ; MOLECULAR-CLONING ; GENE-EXPRESSION ; TRIGLYCERIDE ; RESPONSES ; EXPOSURE ; LIVER |
| 收录类别 | SCI |
| 资助信息 | China Hi-Tech Research and Development Program [2006AA06Z424]; National Natural Science Foundation, China [90608017, 20897013] |
| 语种 | 英语 |
| WOS记录号 | WOS:000263021100003 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7892]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Liwen,Li, Dongliang,Wang, Jianwei,et al. Effects of adrenergic agonists on the extrahepatic expression of vitellogenin Ao1 in heart and brain of the Chinese rare minnow (Gobiocypris rarus)[J]. AQUATIC TOXICOLOGY,2009,91(1):19-25. |
| APA | Ma, Liwen,Li, Dongliang,Wang, Jianwei,He, Jiangyan,&Yin, Zhan.(2009).Effects of adrenergic agonists on the extrahepatic expression of vitellogenin Ao1 in heart and brain of the Chinese rare minnow (Gobiocypris rarus).AQUATIC TOXICOLOGY,91(1),19-25. |
| MLA | Ma, Liwen,et al."Effects of adrenergic agonists on the extrahepatic expression of vitellogenin Ao1 in heart and brain of the Chinese rare minnow (Gobiocypris rarus)".AQUATIC TOXICOLOGY 91.1(2009):19-25. |
入库方式： OAI收割
来源：水生生物研究所
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