Molecular cloning and characterization analysis of immunoglobulin M heavy chain gene in European eel (Anguilla anguilla)
文献类型：期刊论文
| 作者 | Feng Jianjun1,2; Guan Ruizhang1; Lin Peng; Guo Songlin1,2 |
| 刊名 | VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
 |
| 出版日期 | 2009-01-15 |
| 卷号 | 127期号:1-2页码:144-147 |
| 关键词 | Cloning Heavy chain gene Immunoglobulin M European eel Real-time PCR |
| ISSN号 | 0165-2427 |
| 通讯作者 | Guan, RZ, Jimei Univ, Coll Fisheries, 183 Yin Jiang Rd, Jimei 361021, Peoples R China |
| 中文摘要 | In this study, the immunoglobulin M heavy chain gene of European eel (Anguilla anguilla) was cloned and analyzed. The full-length cDNA of the IgM heavy chain gene (GenBank accession no. EF062515) has 2089 nucleotides encoding a putative protein of 581 amino acids. The IgM heavy chain was composed of leader peptide (L), variable domain (VH), CH1, CH2. Hinge, CH3, CH4, and C-terminus and two novel continuous putative N-glycosylation sites were found close to the second cysteine of CH3 in A. anguilla-H1 and A. anguilla-H2. The deduced amino acid sequence of the European eel IgM heavy chain constant region shared similarities to that of the Ladyfish (Elops saurus). Atlantic salmon (Salmo salar), rainbow trout (Oncorhynchus mykiss), Grass carp (Ctenopharingodon idella), Common carp (Cyprinus carpio), Channel catfish (Ictalurus punctatus), and the orange-spotted grouper (Epinephelus coioides) with the identity of 46.1%, 39.7%, 38.9%, 32.4%, 32.3%, 31.7%, and 30.7%, respectively. The highest level of IgM gene expression was observed in the kidney, followed by the spleen, gills, liver, muscle and heart in the apparently healthy European eels. (C) 2008 Elsevier B.V. All rights reserved. |
| 英文摘要 | In this study, the immunoglobulin M heavy chain gene of European eel (Anguilla anguilla) was cloned and analyzed. The full-length cDNA of the IgM heavy chain gene (GenBank accession no. EF062515) has 2089 nucleotides encoding a putative protein of 581 amino acids. The IgM heavy chain was composed of leader peptide (L), variable domain (VH), CH1, CH2. Hinge, CH3, CH4, and C-terminus and two novel continuous putative N-glycosylation sites were found close to the second cysteine of CH3 in A. anguilla-H1 and A. anguilla-H2. The deduced amino acid sequence of the European eel IgM heavy chain constant region shared similarities to that of the Ladyfish (Elops saurus). Atlantic salmon (Salmo salar), rainbow trout (Oncorhynchus mykiss), Grass carp (Ctenopharingodon idella), Common carp (Cyprinus carpio), Channel catfish (Ictalurus punctatus), and the orange-spotted grouper (Epinephelus coioides) with the identity of 46.1%, 39.7%, 38.9%, 32.4%, 32.3%, 31.7%, and 30.7%, respectively. The highest level of IgM gene expression was observed in the kidney, followed by the spleen, gills, liver, muscle and heart in the apparently healthy European eels. (C) 2008 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Immunology; Veterinary Sciences |
| 类目[WOS] | Immunology ; Veterinary Sciences |
| 研究领域[WOS] | Immunology ; Veterinary Sciences |
| 关键词[WOS] | TELEOST FISH ; DISCOVERY ; ISOTYPE |
| 收录类别 | SCI |
| 资助信息 | Agriculture public welfare industry of China [nyhyzx07-043]; program of the Science and Technology Department of Fujian Province [2002N024]; Xiamen Science and Technology Project [3502Z2001244]; Key Laboratory of Science and Technology for Aquaculture and Food Safety ; Fujian Province University [2008J203] |
| 语种 | 英语 |
| WOS记录号 | WOS:000262781300015 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7898]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng Jianjun,Guan Ruizhang,Lin Peng,et al. Molecular cloning and characterization analysis of immunoglobulin M heavy chain gene in European eel (Anguilla anguilla)[J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2009,127(1-2):144-147. |
| APA | Feng Jianjun,Guan Ruizhang,Lin Peng,&Guo Songlin.(2009).Molecular cloning and characterization analysis of immunoglobulin M heavy chain gene in European eel (Anguilla anguilla).VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,127(1-2),144-147. |
| MLA | Feng Jianjun,et al."Molecular cloning and characterization analysis of immunoglobulin M heavy chain gene in European eel (Anguilla anguilla)".VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 127.1-2(2009):144-147. |
入库方式： OAI收割
来源：水生生物研究所
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