Staphylococcus aureus lipoproteins trigger human corneal epithelial innate response through toll-like receptor-2
文献类型：期刊论文
| 作者 | Li, Qiong1,2; Kumar, Ashok1; Gui, Jian-Fang2; Yu, Fu-Shin X.1 |
| 刊名 | MICROBIAL PATHOGENESIS
 |
| 出版日期 | 2008-05-01 |
| 卷号 | 44期号:5页码:426-434 |
| ISSN号 | 0882-4010 |
| 关键词 | Staphylococcus aureus Toll-like receptors Innate resposne Lipoprotein Corneal epithelium |
| 通讯作者 | Yu, FSX, Wayne State Univ, Sch Med, Dept Ophthalmol, Kresge Eye Inst, Detroit, MI 48201 USA |
| 中文摘要 | Bacterial lipoproteins (LP) are a family of cell wall components found in a wide variety of bacteria. In this study, we characterized the response of HUCL, a telomerase-immortalized human corneal epithelial cell (HCEC) line, to LP isolated from Staphylococcus (S) aureus. S. aureus LP (saLP) prepared by Triton X-114 extraction stimulated the activation of NF-kappa B, JNK, and P38 signaling pathways in HUCL cells. The extracts failed to stimulate NF-kappa B activation in HUCL cells after lipoprotein lipase treatment and in cell lines expressing TLR4 or TLR9, but not TLR2, indicating lipoprotein nature of the extracts. saLP induced the up-regulation of a variety of inflammatory cytokines and chemokines (IL-6, IL-8, ICAM-1). antimicrobial molecules (hBD-2, LL-37, and iNOS), and homeostasis genes (Mn-SOD) at both the mRNA level and protein level. Similar inflammatory response to saLP was also observed in primarily cultured HCECs using the production of IL-6 as readout. Moreover, TLR2 neutralizing antibody blocked the saLP-induced secretion of IL-6, IL-8 and hBD2 in HUCL cells. Our findings suggest that saLP activates TLR2 and triggers innate immune response in the cornea to S. aureus infection via production of proinflammatory cytokines and defense molecules. (C) 2007 Elsevier Ltd. All rights reserved. |
| 英文摘要 | Bacterial lipoproteins (LP) are a family of cell wall components found in a wide variety of bacteria. In this study, we characterized the response of HUCL, a telomerase-immortalized human corneal epithelial cell (HCEC) line, to LP isolated from Staphylococcus (S) aureus. S. aureus LP (saLP) prepared by Triton X-114 extraction stimulated the activation of NF-kappa B, JNK, and P38 signaling pathways in HUCL cells. The extracts failed to stimulate NF-kappa B activation in HUCL cells after lipoprotein lipase treatment and in cell lines expressing TLR4 or TLR9, but not TLR2, indicating lipoprotein nature of the extracts. saLP induced the up-regulation of a variety of inflammatory cytokines and chemokines (IL-6, IL-8, ICAM-1). antimicrobial molecules (hBD-2, LL-37, and iNOS), and homeostasis genes (Mn-SOD) at both the mRNA level and protein level. Similar inflammatory response to saLP was also observed in primarily cultured HCECs using the production of IL-6 as readout. Moreover, TLR2 neutralizing antibody blocked the saLP-induced secretion of IL-6, IL-8 and hBD2 in HUCL cells. Our findings suggest that saLP activates TLR2 and triggers innate immune response in the cornea to S. aureus infection via production of proinflammatory cytokines and defense molecules. (C) 2007 Elsevier Ltd. All rights reserved. |
| 学科主题 | Immunology; Microbiology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Immunology ; Microbiology |
| 研究领域[WOS] | Immunology ; Microbiology |
| 关键词[WOS] | CELL-WALL COMPONENTS ; BACTERIAL KERATITIS ; HELICOBACTER-PYLORI ; LIPOTEICHOIC ACID ; SHIFTING TRENDS ; CUTTING EDGE ; PROTEIN-A ; PEPTIDOGLYCAN ; EXPRESSION ; INFECTION |
| 资助信息 | NIH [R01EY14080, EY10869]; Research to Prevent Blindness to the Department of Ophthalmology, Wayne State University School of Medicine |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000262591100008 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7906]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Wayne State Univ, Sch Med, Dept Ophthalmol, Kresge Eye Inst, Detroit, MI 48201 USA 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Qiong,Kumar, Ashok,Gui, Jian-Fang,et al. Staphylococcus aureus lipoproteins trigger human corneal epithelial innate response through toll-like receptor-2[J]. MICROBIAL PATHOGENESIS,2008,44(5):426-434. |
| APA | Li, Qiong,Kumar, Ashok,Gui, Jian-Fang,&Yu, Fu-Shin X..(2008).Staphylococcus aureus lipoproteins trigger human corneal epithelial innate response through toll-like receptor-2.MICROBIAL PATHOGENESIS,44(5),426-434. |
| MLA | Li, Qiong,et al."Staphylococcus aureus lipoproteins trigger human corneal epithelial innate response through toll-like receptor-2".MICROBIAL PATHOGENESIS 44.5(2008):426-434. |
入库方式： OAI收割
来源：水生生物研究所
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