Microcystin-RR induces physiological stress and cell death in the cyanobacterium Aphanizomenon sp DC01 isolated from Lake Dianchi, China
文献类型：期刊论文
| 作者 | Hu, Zhiquan1,2; Li, Dunhai1; Xiao, Bo2; Dauta, Alain3; Liu, Yongding1 |
| 刊名 | FUNDAMENTAL AND APPLIED LIMNOLOGY
 |
| 出版日期 | 2008 |
| 卷号 | 173期号:2页码:111-120 |
| ISSN号 | 1863-9135 |
| 关键词 | Microcystin-RR Aphanizomenon sp DC01 physiological stress cell death ultrastructure |
| 通讯作者 | Liu, YD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China |
| 中文摘要 | The phytoplankton community in Lake Dianchi (Yunnan Province, Southwestern China) is dominated in April by a bloom of Aphanizomenon, that disappears Suddenly and is displaced by a Microcystis bloom in May. The reasons for the rapid bloom disappearance phenomenon and the temporal variability in the composition of phytoplankton assemblages are poorly understood. Cell growth, ultrastructure and physiological changes were examined in cultures of Aphanizomenon sp. DC01 isolated from Lake Dianchi exposed to different closes of rnicrocystin-RR (MC-RR) produced by the Microcystis bloom. MC-RR concentrations above 100 mu g L-1 markedly inhibited the pigment (chlorophyll-a, phycocyanin) synthesis and caused an increase of soluble carbohydrate and protein contents and nitrate reductase activity of toxin-treated blue-green algae. A drastic. reduction in photochemical efficiency of PSII (Fv/Fm) was also found. Morphological examinationn showed that the Aphanizomenon filaments disintegrated and file cells lysed gradually after 48 h Of toxin exposure. Transmission electron microscopy revealed that cellular inclusions of stressed cells almost leaked out completely and the cell membranes were grossly damaged. These findings demonstrate the allelopathic activity of Microcystis aeruginosa inducing physiological stress and cell death of Aphanizomenon sp. DC01 Although the active concentrations of microcystin were rather high, we propose that microcystin may function as allelopathic Substance due to inhomogeneous toxin concentrations close to Microcystis cells. Hence, it may play a role in species Succession of Aphanizomenon and Microcystis in Lake Dianchi. |
| 英文摘要 | The phytoplankton community in Lake Dianchi (Yunnan Province, Southwestern China) is dominated in April by a bloom of Aphanizomenon, that disappears Suddenly and is displaced by a Microcystis bloom in May. The reasons for the rapid bloom disappearance phenomenon and the temporal variability in the composition of phytoplankton assemblages are poorly understood. Cell growth, ultrastructure and physiological changes were examined in cultures of Aphanizomenon sp. DC01 isolated from Lake Dianchi exposed to different closes of rnicrocystin-RR (MC-RR) produced by the Microcystis bloom. MC-RR concentrations above 100 mu g L(-1) markedly inhibited the pigment (chlorophyll-a, phycocyanin) synthesis and caused an increase of soluble carbohydrate and protein contents and nitrate reductase activity of toxin-treated blue-green algae. A drastic. reduction in photochemical efficiency of PSII (Fv/Fm) was also found. Morphological examinationn showed that the Aphanizomenon filaments disintegrated and file cells lysed gradually after 48 h Of toxin exposure. Transmission electron microscopy revealed that cellular inclusions of stressed cells almost leaked out completely and the cell membranes were grossly damaged. These findings demonstrate the allelopathic activity of Microcystis aeruginosa inducing physiological stress and cell death of Aphanizomenon sp. DC01 Although the active concentrations of microcystin were rather high, we propose that microcystin may function as allelopathic Substance due to inhomogeneous toxin concentrations close to Microcystis cells. Hence, it may play a role in species Succession of Aphanizomenon and Microcystis in Lake Dianchi. |
| 学科主题 | Limnology; Marine & Freshwater Biology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Limnology ; Marine & Freshwater Biology |
| 研究领域[WOS] | Marine & Freshwater Biology |
| 关键词[WOS] | DINOFLAGELLATE PERIDINIUM-GATUNENSE ; SYNECHOCOCCUS-ELONGATUS ; GROWTH ; PHOTOSYNTHESIS ; INHIBITION ; SUCCESSION ; TOXINS ; ALGA |
| 资助信息 | National Basic Research Projects [2002CB412300, 2003CB716801]; Huazhong University of Science and Technology [0 124261002] |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000262356500003 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7918]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Wuhan 430072, Peoples R China 2.Huazhong Univ Sci & Technol, Sch Environm Sci & Engn, Wuhan 430074, Peoples R China 3.CNRS UPS INPT, UMR 5245, Equipe InterBio EcoLab, Lab Ecol Fonctionnelle, F-31055 Toulouse, France |
| 推荐引用方式 GB/T 7714 | Hu, Zhiquan,Li, Dunhai,Xiao, Bo,et al. Microcystin-RR induces physiological stress and cell death in the cyanobacterium Aphanizomenon sp DC01 isolated from Lake Dianchi, China[J]. FUNDAMENTAL AND APPLIED LIMNOLOGY,2008,173(2):111-120. |
| APA | Hu, Zhiquan,Li, Dunhai,Xiao, Bo,Dauta, Alain,&Liu, Yongding.(2008).Microcystin-RR induces physiological stress and cell death in the cyanobacterium Aphanizomenon sp DC01 isolated from Lake Dianchi, China.FUNDAMENTAL AND APPLIED LIMNOLOGY,173(2),111-120. |
| MLA | Hu, Zhiquan,et al."Microcystin-RR induces physiological stress and cell death in the cyanobacterium Aphanizomenon sp DC01 isolated from Lake Dianchi, China".FUNDAMENTAL AND APPLIED LIMNOLOGY 173.2(2008):111-120. |
入库方式： OAI收割
来源：水生生物研究所
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