Identification of differential transcript profiles between mutual crossbred embryos of zebrafish (Danio rerio) and Chinese rare minnow (Gobiocypris rarus) by cDNA-AFLP
文献类型：期刊论文
| 作者 | Liu, J.1; Sun, Y. H.1; Wang, Y. W.1,2; Wang, N.1; Pei, D. S.1; Wang, Y. P.1; Hu, W.1; Zhu, Z. Y.1 |
| 刊名 | THERIOGENOLOGY
 |
| 出版日期 | 2008-12-01 |
| 卷号 | 70期号:9页码:1525-1535 |
| ISSN号 | 0093-691X |
| 关键词 | Zebrafish Chinese rare minnow Crossbred embryo cDNA-AFLP Differential expression |
| 通讯作者 | Sun, YH, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | The crosstalk between naive nucleus and maternal factors deposited in egg cytoplasm before zygotic genome activation is crucial for early development. In this study, we utilized two laboratory fishes, zebrafish (Danio rerio) and Chinese rare minnow and Chinese rare minnow (Gobiocypris rarus), to obtain mutual crossbred embroys and examine the interaction between nucleus and egg cytoplasm from different species. Although these two types of crossbred embryos originated from common nuclei, various developmental capacities were gained due to different origins of the egg cytoplasm. Using cDNA amplified fragment length polymorphism (cDNA-AFLP), We Compared transcript profiles between the mutual crossbred embryos at two developmental stages (50%- and 90%-epiholy). Three thousand cDNA fragments were generated in four cDNA pools with 64 primer combinations. All differently displayed transcript-derived fragments (TDFs) were screened by (lot blot hybridization, and the selected sequences were further analyzed by semi-quantitative RT-PCR and quantitative real-time RT-PCR. Compared with ZR embryos, 12 genes were up-regulated and 12 were down-regulated in RZ embryos. The gene fragments were sequenced and subjected to BLASTN analysis. The sequences encoded various proteins which functioned at various levels of proliferation, growth, and development. One gene (ZR6), dramatically down-regulated in RZ embryos, was chosen for loss-of-function study; the knockdown of ZR6 gave rise to the phenotype resembling that of RZ embryos. (c) 2008 Elsevier Inc. All rights reserved. |
| 英文摘要 | The crosstalk between naive nucleus and maternal factors deposited in egg cytoplasm before zygotic genome activation is crucial for early development. In this study, we utilized two laboratory fishes, zebrafish (Danio rerio) and Chinese rare minnow and Chinese rare minnow (Gobiocypris rarus), to obtain mutual crossbred embroys and examine the interaction between nucleus and egg cytoplasm from different species. Although these two types of crossbred embryos originated from common nuclei, various developmental capacities were gained due to different origins of the egg cytoplasm. Using cDNA amplified fragment length polymorphism (cDNA-AFLP), We Compared transcript profiles between the mutual crossbred embryos at two developmental stages (50%- and 90%-epiholy). Three thousand cDNA fragments were generated in four cDNA pools with 64 primer combinations. All differently displayed transcript-derived fragments (TDFs) were screened by (lot blot hybridization, and the selected sequences were further analyzed by semi-quantitative RT-PCR and quantitative real-time RT-PCR. Compared with ZR embryos, 12 genes were up-regulated and 12 were down-regulated in RZ embryos. The gene fragments were sequenced and subjected to BLASTN analysis. The sequences encoded various proteins which functioned at various levels of proliferation, growth, and development. One gene (ZR6), dramatically down-regulated in RZ embryos, was chosen for loss-of-function study; the knockdown of ZR6 gave rise to the phenotype resembling that of RZ embryos. (c) 2008 Elsevier Inc. All rights reserved. |
| 学科主题 | Reproductive Biology; Veterinary Sciences |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Reproductive Biology ; Veterinary Sciences |
| 研究领域[WOS] | Reproductive Biology ; Veterinary Sciences |
| 关键词[WOS] | CELL NUCLEAR TRANSFER ; GENE-EXPRESSION ; ENUCLEATED EGGS ; XENOMITOCHONDRIAL CYBRIDS ; SACCHAROMYCES-CEREVISIAE ; GENOME ; MITOCHONDRIA ; DEFICIENCY ; STABILITY ; IMPACT |
| 资助信息 | State Key Fundamental Research of China [2004CB117406]; National Natural Science Foundation of China [30771100] |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000261285000016 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7938]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, J.,Sun, Y. H.,Wang, Y. W.,et al. Identification of differential transcript profiles between mutual crossbred embryos of zebrafish (Danio rerio) and Chinese rare minnow (Gobiocypris rarus) by cDNA-AFLP[J]. THERIOGENOLOGY,2008,70(9):1525-1535. |
| APA | Liu, J..,Sun, Y. H..,Wang, Y. W..,Wang, N..,Pei, D. S..,...&Zhu, Z. Y..(2008).Identification of differential transcript profiles between mutual crossbred embryos of zebrafish (Danio rerio) and Chinese rare minnow (Gobiocypris rarus) by cDNA-AFLP.THERIOGENOLOGY,70(9),1525-1535. |
| MLA | Liu, J.,et al."Identification of differential transcript profiles between mutual crossbred embryos of zebrafish (Danio rerio) and Chinese rare minnow (Gobiocypris rarus) by cDNA-AFLP".THERIOGENOLOGY 70.9(2008):1525-1535. |
入库方式： OAI收割
来源：水生生物研究所
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