Establishment of a Chinese sturgeon Acipenser sinensis tail-fin cell line and its susceptibility to frog iridovirus
文献类型：期刊论文
| 作者 | Zhou, G. Z.; Gui, L.; Li, Z. Q.; Yuan, X. P.; Zhang, Q. Y. |
| 刊名 | JOURNAL OF FISH BIOLOGY
 |
| 出版日期 | 2008-12-01 |
| 卷号 | 73期号:8页码:2058-2067 |
| ISSN号 | 0022-1112 |
| 关键词 | chromosome cytopathic effect electron microscope freshwater fish immunofluorescence karyotype |
| 通讯作者 | Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Chinese sturgeon Acipenser sinensis, a cartilaginous ganoid, is a 'living fossil' on a deeply isolated evolutionary branch. A cell line was established from Chinese sturgeon tail-fin tissue (CSTF) . These epithelial CSTF cells grew well in Dulbecco's modified Eagle's medium at 25 degrees C. Karyotypic analysis revealed a normal diploid karyotype with 2n = 264 and large numbers of punctate chromosomes. A strain of frog iridoviruses [Rana grylio virus (RGV)] was used to test the susceptibility of this cell line to infection. Infection was confirmed by cytopathic effect, immunofluorescence and electron-microscope observations, which detected the viral antigens or particles in the cytoplasm of RGV-infected cells. Molecular analysis further suggested that c. 550 bp DNA fragment could be cloned from the RGV-infected CSTF cells' DNA with major capsid protein gene polymerase chain reaction primers. Furthermore, after transfection with pEGFP vector DNA, the CSTF cell line produced significant fluorescent signals indicating its utility in exogenous studies. |
| 英文摘要 | Chinese sturgeon Acipenser sinensis, a cartilaginous ganoid, is a 'living fossil' on a deeply isolated evolutionary branch. A cell line was established from Chinese sturgeon tail-fin tissue (CSTF) . These epithelial CSTF cells grew well in Dulbecco's modified Eagle's medium at 25 degrees C. Karyotypic analysis revealed a normal diploid karyotype with 2n = 264 and large numbers of punctate chromosomes. A strain of frog iridoviruses [Rana grylio virus (RGV)] was used to test the susceptibility of this cell line to infection. Infection was confirmed by cytopathic effect, immunofluorescence and electron-microscope observations, which detected the viral antigens or particles in the cytoplasm of RGV-infected cells. Molecular analysis further suggested that c. 550 bp DNA fragment could be cloned from the RGV-infected CSTF cells' DNA with major capsid protein gene polymerase chain reaction primers. Furthermore, after transfection with pEGFP vector DNA, the CSTF cell line produced significant fluorescent signals indicating its utility in exogenous studies. |
| 学科主题 | Fisheries; Marine & Freshwater Biology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Marine & Freshwater Biology |
| 研究领域[WOS] | Fisheries ; Marine & Freshwater Biology |
| 关键词[WOS] | RANA-GRYLIO VIRUS ; WHITE STURGEON ; INFECTION |
| 资助信息 | National Major Basic Research Programme [2004CB117403]; National 863 High Technology Research Foundation of China [2006AA09Z445, 2006AA100309, 20060110A4013]; National Natural Science Foundation of China [30671616, U0631008]; Project of Chinese Academy of Sciences [KSCX2-YW-N-021]; Key Technology R & D Programme of China [2006BAD03B05] |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000261252300014 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7954]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, G. Z.,Gui, L.,Li, Z. Q.,et al. Establishment of a Chinese sturgeon Acipenser sinensis tail-fin cell line and its susceptibility to frog iridovirus[J]. JOURNAL OF FISH BIOLOGY,2008,73(8):2058-2067. |
| APA | Zhou, G. Z.,Gui, L.,Li, Z. Q.,Yuan, X. P.,&Zhang, Q. Y..(2008).Establishment of a Chinese sturgeon Acipenser sinensis tail-fin cell line and its susceptibility to frog iridovirus.JOURNAL OF FISH BIOLOGY,73(8),2058-2067. |
| MLA | Zhou, G. Z.,et al."Establishment of a Chinese sturgeon Acipenser sinensis tail-fin cell line and its susceptibility to frog iridovirus".JOURNAL OF FISH BIOLOGY 73.8(2008):2058-2067. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。