Molecular cloning and expression characterization of ApoC-I in the orange-spotted grouper
文献类型：期刊论文
| 作者 | Wang, Y.; Zhou, L.; Li, Z.; Gui, J. F. |
| 刊名 | FISH PHYSIOLOGY AND BIOCHEMISTRY
 |
| 出版日期 | 2008-12-01 |
| 卷号 | 34期号:4页码:339-348 |
| 关键词 | Apolipoproteins ApoC-I Grouper Yolk syncytial layer |
| ISSN号 | 0920-1742 |
| 通讯作者 | Gui, JF, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan Ctr Dev Biol,Grad Sch, Wuhan 430072, Peoples R China |
| 中文摘要 | Endogenous yolk nutrients are crucial for embryo and larval development in fish, but developmental behavior of the genes that control yolk utilization remains unknown. Apolipoproteins have been shown to play important roles in lipid transport and uptake through the circulation system. In this study, EcApoC-I, the first cloned ApoC-I in teleosts, has been screened from pituitary cDNA library of female orange-spotted grouper (Epinephelus coioides), and the deduced amino acid sequence shows 43.5% identity to one zebrafish (Danio rerio) hypothetical protein similar to ApoC-I, and 21.2%, 21.7%, 22.5%, 20%, and 22.5% identities to Apo C-I of human (Homo sapiens), house mouse (Mus musculus), common tree shrew (Tupaia glis), dog (Canis lupus familiaris) and hamadryas baboon (Papio hamadryas), respectively. Although the sequence identity is low, amphipathic alpha-helices with the potential to bind to lipid were predicted to exist in the EcApoC-I. RT-PCR analysis revealed that it was first transcribed in gastrula embryos and maintained a relatively stable expression level during the following embryogenesis. During embryonic and early larval development, a very high level of EcApoC-I expression was in the yolk syncytial layer, indicating that it plays a significant role in yolk degradation and transfers nutrition to the embryo and early larva. By the day 7 after hatching, EcApoC-I transcripts were observed in brain. In adult, EcApoC-I mRNA was detected abundantly in brain and gonad. In transitional gonads, the EcApoC-I expression is restricted to the germ cells. The data suggested that EcApoC-I might play an important role in brain and gonad morphogenesis and growth. |
| 英文摘要 | Endogenous yolk nutrients are crucial for embryo and larval development in fish, but developmental behavior of the genes that control yolk utilization remains unknown. Apolipoproteins have been shown to play important roles in lipid transport and uptake through the circulation system. In this study, EcApoC-I, the first cloned ApoC-I in teleosts, has been screened from pituitary cDNA library of female orange-spotted grouper (Epinephelus coioides), and the deduced amino acid sequence shows 43.5% identity to one zebrafish (Danio rerio) hypothetical protein similar to ApoC-I, and 21.2%, 21.7%, 22.5%, 20%, and 22.5% identities to Apo C-I of human (Homo sapiens), house mouse (Mus musculus), common tree shrew (Tupaia glis), dog (Canis lupus familiaris) and hamadryas baboon (Papio hamadryas), respectively. Although the sequence identity is low, amphipathic alpha-helices with the potential to bind to lipid were predicted to exist in the EcApoC-I. RT-PCR analysis revealed that it was first transcribed in gastrula embryos and maintained a relatively stable expression level during the following embryogenesis. During embryonic and early larval development, a very high level of EcApoC-I expression was in the yolk syncytial layer, indicating that it plays a significant role in yolk degradation and transfers nutrition to the embryo and early larva. By the day 7 after hatching, EcApoC-I transcripts were observed in brain. In adult, EcApoC-I mRNA was detected abundantly in brain and gonad. In transitional gonads, the EcApoC-I expression is restricted to the germ cells. The data suggested that EcApoC-I might play an important role in brain and gonad morphogenesis and growth. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology; Fisheries; Physiology |
| 类目[WOS] | Biochemistry & Molecular Biology ; Fisheries ; Physiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Fisheries ; Physiology |
| 关键词[WOS] | YOLK SYNCYTIAL LAYER ; LOW-DENSITY LIPOPROTEINS ; APOLIPOPROTEIN MULTIGENE FAMILY ; TURBOT SCOPHTHALMUS-MAXIMUS ; FARIO-TRUTTA L ; EPINEPHELUS-COIOIDES ; C-I ; PLASMA APOLIPOPROTEINS ; SEX REVERSAL ; DIFFERENTIAL EXPRESSION |
| 收录类别 | SCI |
| 资助信息 | National Major Basic Research Program [2004CB117401]; National 863 High Technology Research Program [2006AA09Z439]; Chinese Academy of Sciences [KSCX2-YW-N-020] |
| 语种 | 英语 |
| WOS记录号 | WOS:000260512800006 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7968]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan Ctr Dev Biol,Grad Sch, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Y.,Zhou, L.,Li, Z.,et al. Molecular cloning and expression characterization of ApoC-I in the orange-spotted grouper[J]. FISH PHYSIOLOGY AND BIOCHEMISTRY,2008,34(4):339-348. |
| APA | Wang, Y.,Zhou, L.,Li, Z.,&Gui, J. F..(2008).Molecular cloning and expression characterization of ApoC-I in the orange-spotted grouper.FISH PHYSIOLOGY AND BIOCHEMISTRY,34(4),339-348. |
| MLA | Wang, Y.,et al."Molecular cloning and expression characterization of ApoC-I in the orange-spotted grouper".FISH PHYSIOLOGY AND BIOCHEMISTRY 34.4(2008):339-348. |
入库方式： OAI收割
来源：水生生物研究所
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