Comparative study and expression analysis of the interferon gamma gene locus cytokines in Xenopus tropicalis
文献类型：期刊论文
| 作者 | Qi, Z. T.1,2,3; Nie, P.1,2 |
| 刊名 | IMMUNOGENETICS
 |
| 出版日期 | 2008-11-01 |
| 卷号 | 60期号:11页码:699-710 |
| 关键词 | Interleukin 22 Interleukin 26 Interferon gamma Xenopus tropicalis |
| ISSN号 | 0093-7711 |
| 通讯作者 | Nie, P, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China |
| 中文摘要 | Using bioinformatics approach, the genome locus containing interleukin (IL)-22, IL-26, and interferon gamma (IFN-gamma) genes has been identified in the amphibian, Xenopus tropicalis. Like that in other vertebrates such as fish, birds, and mammals, the Xenopus IL-22, IL-26, and IFN-gamma are clustered in the same chromosome and the adjacent genes are conserved. The genomic structures of the Xenopus IL-22, IL-26, and IFN-gamma gene were identical to that of their mammalian counterparts. The Xenopus IL-22 and IL-26 genes contained five exons and four introns while the Xenopus IFN-gamma gene consisted of four exons and three introns. The Xenopus IL-22, IL-26, and IFN-gamma share 14.1-41.6%, 14.6-31.2%, and 23.7-36.5% identity to their counterparts in other species, respectively. Reverse-transcription polymerase chain reaction (PCR) and real-time quantitative PCR analyses revealed that the expression of IL-22, IL-26, and IFN-gamma genes was significantly upregulated after simulation with bacterial polyliposaccharide and/or synthetic double-stranded poly(I:C), suggesting these cytokines like those in other vertebrates play an important role in regulating immune response in Xenopus. |
| 英文摘要 | Using bioinformatics approach, the genome locus containing interleukin (IL)-22, IL-26, and interferon gamma (IFN-gamma) genes has been identified in the amphibian, Xenopus tropicalis. Like that in other vertebrates such as fish, birds, and mammals, the Xenopus IL-22, IL-26, and IFN-gamma are clustered in the same chromosome and the adjacent genes are conserved. The genomic structures of the Xenopus IL-22, IL-26, and IFN-gamma gene were identical to that of their mammalian counterparts. The Xenopus IL-22 and IL-26 genes contained five exons and four introns while the Xenopus IFN-gamma gene consisted of four exons and three introns. The Xenopus IL-22, IL-26, and IFN-gamma share 14.1-41.6%, 14.6-31.2%, and 23.7-36.5% identity to their counterparts in other species, respectively. Reverse-transcription polymerase chain reaction (PCR) and real-time quantitative PCR analyses revealed that the expression of IL-22, IL-26, and IFN-gamma genes was significantly upregulated after simulation with bacterial polyliposaccharide and/or synthetic double-stranded poly(I:C), suggesting these cytokines like those in other vertebrates play an important role in regulating immune response in Xenopus. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Genetics & Heredity; Immunology |
| 类目[WOS] | Genetics & Heredity ; Immunology |
| 研究领域[WOS] | Genetics & Heredity ; Immunology |
| 关键词[WOS] | INTERLEUKIN (IL)-22 ; T-CELLS ; TRANSCRIPTION FACTOR ; REGULATORY ELEMENTS ; SEQUENCE ALIGNMENT ; IFN-GAMMA ; IDENTIFICATION ; HOMOLOG ; IL-10 ; RECEPTOR |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China (NSFC) [U0631010]; Government of Guangdong Province ; NSFC [30711130225]; Royal Society of Edinburgh |
| 语种 | 英语 |
| WOS记录号 | WOS:000260183000007 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7978]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Lab Fish Dis, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qi, Z. T.,Nie, P.. Comparative study and expression analysis of the interferon gamma gene locus cytokines in Xenopus tropicalis[J]. IMMUNOGENETICS,2008,60(11):699-710. |
| APA | Qi, Z. T.,&Nie, P..(2008).Comparative study and expression analysis of the interferon gamma gene locus cytokines in Xenopus tropicalis.IMMUNOGENETICS,60(11),699-710. |
| MLA | Qi, Z. T.,et al."Comparative study and expression analysis of the interferon gamma gene locus cytokines in Xenopus tropicalis".IMMUNOGENETICS 60.11(2008):699-710. |
入库方式： OAI收割
来源：水生生物研究所
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