Microsatellite marker isolation and cultured strain identification in Carassius auratus gibelio
文献类型：期刊论文
| 作者 | Guo, Wei1,2,3; Gui, Jian-Fang1 |
| 刊名 | AQUACULTURE INTERNATIONAL
 |
| 出版日期 | 2008-12-01 |
| 卷号 | 16期号:6页码:497-510 |
| 关键词 | Gibel carp Microsatellite Gynogenesis Polyploidy Molecular marker |
| ISSN号 | 0967-6120 |
| 通讯作者 | Gui, JF, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Microsatellites have become the preferred molecular markers for strain selection and genetic breeding in fish. In this study a total of 105 microsatellites were isolated and identified in gibel carp (Carassius auratus gibelio) by microsatellite sequence searches in GenBank and other databases and by screening and sequencing of positive clones from the genomic library enriched for AG and GATA repeats. Moreover, nineteen microsatellites were randomly selected to design locus-specific primer pairs, and these were successfully used to identify and discriminate different cultured strains of gibel carp including strains A, D, L, and F. Three different types of microsatellite pattern were distinguished by the number and length of fragments amplified from the 19 primer pairs, and some microsatellite primer pairs were found to produce different microsatellite patterns among strains and strain-specific fragments. In addition, some duplicated alleles were also detected in two microsatellite patterns. Therefore, the current study provides direct molecular markers to discriminate among different cultured strains for selective breeding and aquaculture practice of gibel carp. |
| 英文摘要 | Microsatellites have become the preferred molecular markers for strain selection and genetic breeding in fish. In this study a total of 105 microsatellites were isolated and identified in gibel carp (Carassius auratus gibelio) by microsatellite sequence searches in GenBank and other databases and by screening and sequencing of positive clones from the genomic library enriched for AG and GATA repeats. Moreover, nineteen microsatellites were randomly selected to design locus-specific primer pairs, and these were successfully used to identify and discriminate different cultured strains of gibel carp including strains A, D, L, and F. Three different types of microsatellite pattern were distinguished by the number and length of fragments amplified from the 19 primer pairs, and some microsatellite primer pairs were found to produce different microsatellite patterns among strains and strain-specific fragments. In addition, some duplicated alleles were also detected in two microsatellite patterns. Therefore, the current study provides direct molecular markers to discriminate among different cultured strains for selective breeding and aquaculture practice of gibel carp. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Fisheries |
| 类目[WOS] | Fisheries |
| 研究领域[WOS] | Fisheries |
| 关键词[WOS] | SILVER CRUCIAN CARP ; MOLECULAR MARKERS ; BLOCH ; DNA ; CLONES ; SEQUENCES ; ORIGIN ; GENOME ; LOCI |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [30630050]; National Major Basic Research Program [2004CB117406]; National Tackle Key Problem Program [2006BAD01A1206] |
| 语种 | 英语 |
| WOS记录号 | WOS:000260368100002 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7980]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.Fuzhou Univ, Inst Biol & Med Technol, Fuzhou 350001, Peoples R China |
| 推荐引用方式 GB/T 7714 | Guo, Wei,Gui, Jian-Fang. Microsatellite marker isolation and cultured strain identification in Carassius auratus gibelio[J]. AQUACULTURE INTERNATIONAL,2008,16(6):497-510. |
| APA | Guo, Wei,&Gui, Jian-Fang.(2008).Microsatellite marker isolation and cultured strain identification in Carassius auratus gibelio.AQUACULTURE INTERNATIONAL,16(6),497-510. |
| MLA | Guo, Wei,et al."Microsatellite marker isolation and cultured strain identification in Carassius auratus gibelio".AQUACULTURE INTERNATIONAL 16.6(2008):497-510. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。