Bacterial diversity in activated sludge from a consecutively aerated submerged membrane bioreactor treating domestic wastewater
文献类型：期刊论文
| 作者 | Du Cheng1,2; Wu Zhenbin1; Xiao Enrong1,2; Zhou Qiaohong1; Cheng Shuiping1; Liang Wei1; He Feng1 |
| 刊名 | JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA
 |
| 出版日期 | 2008 |
| 卷号 | 20期号:10页码:1210-1217 |
| 关键词 | bacterial diversity restriction fragment length polymorphism (RFLP) submerged membrane bioreactor (SMBR) 16S rDNA clone library |
| ISSN号 | 1001-0742 |
| 通讯作者 | Wu, ZB, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | The bacterial diversity of activated sludge from submerged membrane bioreactor (SMBR) was investigated. A 16S rDNA clone library was generated, and 150 clones were screened using restriction fragment length polymorphism (RFLP). Of the screened clones, almost full-length 16S rDNA sequences of 64 clones were sequenced. Phylogenetic tree was constructed with a database containing clone sequences from this study and bacterial rDNA sequences from NCB1 for identification purposes. The 90.6% of the clones were affiliated with the two phyla Bacteroidetes (50%) and Proteobacteria (40%), and beta-, -gamma-, and delta-Proteobacteria accounted for 7.8%, 28.1%, and 4.7%, respectively. Minor portions were affiliated with the Actinobacteria and Firmicutes (both 3.1%). Only 6 out of 64 16S rDNA sequences exhibited similarities of more than 97% to classified bacterial species, which indicated that a substantial fraction of the clone sequences were derived from unknown taxa. Rarefaction analysis of operational taxonomic units (orrUs) clusters demonstrated that 150 clones screened were still insufficient to describe the whole bacterial diversity. Measurement of water quality parameter demonstrated that performance of the SMBR maintained high level, and the SMBR system remained stable during this study. |
| 英文摘要 | The bacterial diversity of activated sludge from submerged membrane bioreactor (SMBR) was investigated. A 16S rDNA clone library was generated, and 150 clones were screened using restriction fragment length polymorphism (RFLP). Of the screened clones, almost full-length 16S rDNA sequences of 64 clones were sequenced. Phylogenetic tree was constructed with a database containing clone sequences from this study and bacterial rDNA sequences from NCB1 for identification purposes. The 90.6% of the clones were affiliated with the two phyla Bacteroidetes (50%) and Proteobacteria (40%), and beta-, -gamma-, and delta-Proteobacteria accounted for 7.8%, 28.1%, and 4.7%, respectively. Minor portions were affiliated with the Actinobacteria and Firmicutes (both 3.1%). Only 6 out of 64 16S rDNA sequences exhibited similarities of more than 97% to classified bacterial species, which indicated that a substantial fraction of the clone sequences were derived from unknown taxa. Rarefaction analysis of operational taxonomic units (orrUs) clusters demonstrated that 150 clones screened were still insufficient to describe the whole bacterial diversity. Measurement of water quality parameter demonstrated that performance of the SMBR maintained high level, and the SMBR system remained stable during this study. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Environmental Sciences |
| 类目[WOS] | Environmental Sciences |
| 研究领域[WOS] | Environmental Sciences & Ecology |
| 关键词[WOS] | MICROBIAL DIVERSITY ; NITROGEN REMOVAL ; 16S RDNA ; COMMUNITY ; IDENTIFICATION ; POPULATIONS ; SEDIMENTS ; GENUS ; SOIL |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China [39925007]; HiTech Research and Development Program (863) of China [2002AA601021]; Innovation Program of Chinese Academy of Sciences [KSCX2-SW-102] |
| 语种 | 英语 |
| WOS记录号 | WOS:000260099600009 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/7982]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Du Cheng,Wu Zhenbin,Xiao Enrong,et al. Bacterial diversity in activated sludge from a consecutively aerated submerged membrane bioreactor treating domestic wastewater[J]. JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,2008,20(10):1210-1217. |
| APA | Du Cheng.,Wu Zhenbin.,Xiao Enrong.,Zhou Qiaohong.,Cheng Shuiping.,...&He Feng.(2008).Bacterial diversity in activated sludge from a consecutively aerated submerged membrane bioreactor treating domestic wastewater.JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA,20(10),1210-1217. |
| MLA | Du Cheng,et al."Bacterial diversity in activated sludge from a consecutively aerated submerged membrane bioreactor treating domestic wastewater".JOURNAL OF ENVIRONMENTAL SCIENCES-CHINA 20.10(2008):1210-1217. |
入库方式： OAI收割
来源：水生生物研究所
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