Identification of differentially expressed immune-relevant genes in Chinese soft-shelled turtle (Trionyx sinensis) infected with Aeromonas hydrophila
文献类型：期刊论文
| 作者 | Zhou, Xiuxia1,2; Guo, Qionglin1; Dai, Heping1 |
| 刊名 | VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
 |
| 出版日期 | 2008-09-15 |
| 卷号 | 125期号:1-2页码:82-91 |
| 关键词 | immune-relevant gene Chinese soft-shelled turtle Aeromonas hydrophila suppression subtractive hybridization (SSH) subtractive cDNA library |
| ISSN号 | 0165-2427 |
| 通讯作者 | Guo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | Expressed sequence tag (EST) analysis is an efficient tool for gene discovery and profiling gene expression. Aeromonas hydrophila, a ubiquitous waterborne bacterium, is one of the most frequent pathogens isolated from diseased aquatic organisms. In order to understand the molecular mechanism of anti-bacteria immune response in reptile, we have investigated the differentially expressed genes in Chinese soft-shelled turtle (Trionyx sinensis) experimentally infected with A. hydrophila by suppression subtractive hybridization (SSH). Forty-two genes were identified from more than 200 clones, of which 25 genes are found for the first time in reptiles, and classified into 6 categories: 18 in defense/immunity. 4 in catalysis, 2 in retrotransposon; 2 in cell signal transduction, 5 in cell metabolism, 10 in protein expression, and 1 in cell structure. Of the 42 differentially expressed genes, 6 genes, IL-8, serum amyloid A (SAA), CD9, CD59, activating transcription factor 4 (ATF4) and cathepsin L genes, were further observed to be up-regulated in the infected turtles by virtual Northern hybridization and RT-PCR assays. (C) 2008 Elsevier B.V. All rights reserved. |
| 英文摘要 | Expressed sequence tag (EST) analysis is an efficient tool for gene discovery and profiling gene expression. Aeromonas hydrophila, a ubiquitous waterborne bacterium, is one of the most frequent pathogens isolated from diseased aquatic organisms. In order to understand the molecular mechanism of anti-bacteria immune response in reptile, we have investigated the differentially expressed genes in Chinese soft-shelled turtle (Trionyx sinensis) experimentally infected with A. hydrophila by suppression subtractive hybridization (SSH). Forty-two genes were identified from more than 200 clones, of which 25 genes are found for the first time in reptiles, and classified into 6 categories: 18 in defense/immunity. 4 in catalysis, 2 in retrotransposon; 2 in cell signal transduction, 5 in cell metabolism, 10 in protein expression, and 1 in cell structure. Of the 42 differentially expressed genes, 6 genes, IL-8, serum amyloid A (SAA), CD9, CD59, activating transcription factor 4 (ATF4) and cathepsin L genes, were further observed to be up-regulated in the infected turtles by virtual Northern hybridization and RT-PCR assays. (C) 2008 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Immunology; Veterinary Sciences |
| 类目[WOS] | Immunology ; Veterinary Sciences |
| 研究领域[WOS] | Immunology ; Veterinary Sciences |
| 关键词[WOS] | PHYLOGENETICALLY ANCIENT REPTILE ; UNFOLDED PROTEIN RESPONSE ; ENDOPLASMIC-RETICULUM ; ENDOTHELIAL-CELLS ; VIRULENCE FACTORS ; IMMUNOGLOBULIN ; MHC ; TUATARA ; CLONING ; CAIMAN |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China (Nos. 30070588 and 30371091) [30070588, 30371091] |
| 语种 | 英语 |
| WOS记录号 | WOS:000259313300008 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8004]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Xiuxia,Guo, Qionglin,Dai, Heping. Identification of differentially expressed immune-relevant genes in Chinese soft-shelled turtle (Trionyx sinensis) infected with Aeromonas hydrophila[J]. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,2008,125(1-2):82-91. |
| APA | Zhou, Xiuxia,Guo, Qionglin,&Dai, Heping.(2008).Identification of differentially expressed immune-relevant genes in Chinese soft-shelled turtle (Trionyx sinensis) infected with Aeromonas hydrophila.VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY,125(1-2),82-91. |
| MLA | Zhou, Xiuxia,et al."Identification of differentially expressed immune-relevant genes in Chinese soft-shelled turtle (Trionyx sinensis) infected with Aeromonas hydrophila".VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY 125.1-2(2008):82-91. |
入库方式： OAI收割
来源：水生生物研究所
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