Identification and characterization of a novel envelope protein in Rana grylio virus
文献类型：期刊论文
| 作者 | Zhao, Zhe; Ke, Fei; Huang, You-Hua; Zhao, Jiu-Gang; Gui, Jan-Fang; Zhang, Qi-Ya |
| 刊名 | JOURNAL OF GENERAL VIROLOGY
 |
| 出版日期 | 2008-08-01 |
| 卷号 | 89页码:1866-1872 |
| ISSN号 | 0022-1317 |
| 关键词 | MAJOR CAPSID PROTEIN SWINE-FEVER VIRUS FISH CELL-LINE VACCINIA-VIRUS FAMILY IRIDOVIRIDAE GENE-EXPRESSION DNA MICROARRAYS VIRION ENVELOPE ENTRY MORPHOGENESIS |
| 通讯作者 | Zhang, QY, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Viral envelope proteins have been proposed to play significant roles in virus infection and assembly. In this study, an envelope protein gene, 53R, was cloned and characterized from Rana grylio virus (RGV), a member of the family Iridoviridae. Database searches found its homologues in all sequenced iricloviruses, and sequence alignment revealed several conserved structural features shared by virus capsid or envelope proteins: a myristoylation site, two predicted transmembrane domains and two invariant cysteine residues. Subsequently, RT-PCR and Western blot detection revealed that the transcripts encoding RGV 53R and the protein itself appeared late during infection of fathead minnow cells and that their appearance was blocked by viral DNA replication inhibitor, indicating that RGV 53R is a late expression gene. Moreover, immunofluorescence localization found an association of 53R with virus factories in RGV-infected cells, and this association was further confirmed by expressing a 53R-GFP fusion protein in pEGFP-N3/53R-transfected cells. Furthermore, detergent extraction and Western blot detection confirmed that RGV 53R was associated with virion membrane. Therefore, the current data suggest that RGV 53R is a novel viral envelope protein and that it may play an important role in virus assembly. This is thought to be the first report on a viral envelope protein that is conserved in all sequenced iridoviruses. |
| 英文摘要 | Viral envelope proteins have been proposed to play significant roles in virus infection and assembly. In this study, an envelope protein gene, 53R, was cloned and characterized from Rana grylio virus (RGV), a member of the family Iridoviridae. Database searches found its homologues in all sequenced iricloviruses, and sequence alignment revealed several conserved structural features shared by virus capsid or envelope proteins: a myristoylation site, two predicted transmembrane domains and two invariant cysteine residues. Subsequently, RT-PCR and Western blot detection revealed that the transcripts encoding RGV 53R and the protein itself appeared late during infection of fathead minnow cells and that their appearance was blocked by viral DNA replication inhibitor, indicating that RGV 53R is a late expression gene. Moreover, immunofluorescence localization found an association of 53R with virus factories in RGV-infected cells, and this association was further confirmed by expressing a 53R-GFP fusion protein in pEGFP-N3/53R-transfected cells. Furthermore, detergent extraction and Western blot detection confirmed that RGV 53R was associated with virion membrane. Therefore, the current data suggest that RGV 53R is a novel viral envelope protein and that it may play an important role in virus assembly. This is thought to be the first report on a viral envelope protein that is conserved in all sequenced iridoviruses. |
| 学科主题 | Biotechnology & Applied Microbiology; Virology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology ; Virology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology ; Virology |
| 关键词[WOS] | MAJOR CAPSID PROTEIN ; SWINE-FEVER VIRUS ; FISH CELL-LINE ; VACCINIA-VIRUS ; FAMILY IRIDOVIRIDAE ; GENE-EXPRESSION ; DNA MICROARRAYS ; VIRION ENVELOPE ; ENTRY ; MORPHOGENESIS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000258301800009 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8040]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Zhe,Ke, Fei,Huang, You-Hua,et al. Identification and characterization of a novel envelope protein in Rana grylio virus[J]. JOURNAL OF GENERAL VIROLOGY,2008,89:1866-1872. |
| APA | Zhao, Zhe,Ke, Fei,Huang, You-Hua,Zhao, Jiu-Gang,Gui, Jan-Fang,&Zhang, Qi-Ya.(2008).Identification and characterization of a novel envelope protein in Rana grylio virus.JOURNAL OF GENERAL VIROLOGY,89,1866-1872. |
| MLA | Zhao, Zhe,et al."Identification and characterization of a novel envelope protein in Rana grylio virus".JOURNAL OF GENERAL VIROLOGY 89(2008):1866-1872. |
入库方式： OAI收割
来源：水生生物研究所
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