Tissue distribution and depuration of the extracted hepatotoxic cyanotoxin microcystins in crucian carp (Carassius carassius) intraperitoneally injected at a sublethal dose
文献类型：期刊论文
| 作者 | Lei, Hehua; Xie, Ping; Chen, Jun; Liang, Gaodao; Yu, Ting; Jiang, Yan |
| 刊名 | THESCIENTIFICWORLDJOURNAL
 |
| 出版日期 | 2008 |
| 卷号 | 8期号:0页码:713-719 |
| 关键词 | microcystins intraperitoneal injection crucian carp tissue distribution depuration |
| ISSN号 | 1537-744X |
| 通讯作者 | Xie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China |
| 中文摘要 | An acute toxicity experiment was conducted by intraperitoneal injection with a sublethal dose of extracted microcystins (MCs), 50 mu g MC-LR (where L = leucine and R = arginine) equivalent/kg body weight (BW), to examine tissue distribution and depuration of MCs in crucian carp (Carassius carassius). Liver to body weight ratio increased at 3, 12, 24, and 48 h postinjection compared with that at 0 h (p < 0.05). MC concentrations in various tissues and aquaria water were analyzed at 1, 3, 12, 24, 48, and 168 h postinjection using liquid chromatography coupled with mass spectrometry (LC-MS). The highest concentration of MCs (MC-RR + MC-LR) was found in blood, 2 -270 ng/g dry weight (DW), followed by heart (3 -100 ng/g DW) and kidney (13 -88 ng/g DW). MC levels were relatively low in liver, gonad, intestine, spleen, and brain. MC contents in gills, gallbladder, and muscle were below the limit of detection. Significant negative correlation was present between MC-RR concentration in blood and that in kidney, confirming that blood was important in the transportation of MC-RR to kidney for excretion. Rapid accumulation and slow degradation of MCs were observed in gonad, liver, intestine, spleen, and brain. Only 0.07% of injected MCs were detected in liver. The recovery of MCs in liver of crucian carp seemed to be dose dependent. |
| 英文摘要 | An acute toxicity experiment was conducted by intraperitoneal injection with a sublethal dose of extracted microcystins (MCs), 50 mu g MC-LR (where L = leucine and R = arginine) equivalent/kg body weight (BW), to examine tissue distribution and depuration of MCs in crucian carp (Carassius carassius). Liver to body weight ratio increased at 3, 12, 24, and 48 h postinjection compared with that at 0 h (p < 0.05). MC concentrations in various tissues and aquaria water were analyzed at 1, 3, 12, 24, 48, and 168 h postinjection using liquid chromatography coupled with mass spectrometry (LC-MS). The highest concentration of MCs (MC-RR + MC-LR) was found in blood, 2 -270 ng/g dry weight (DW), followed by heart (3 -100 ng/g DW) and kidney (13 -88 ng/g DW). MC levels were relatively low in liver, gonad, intestine, spleen, and brain. MC contents in gills, gallbladder, and muscle were below the limit of detection. Significant negative correlation was present between MC-RR concentration in blood and that in kidney, confirming that blood was important in the transportation of MC-RR to kidney for excretion. Rapid accumulation and slow degradation of MCs were observed in gonad, liver, intestine, spleen, and brain. Only 0.07% of injected MCs were detected in liver. The recovery of MCs in liver of crucian carp seemed to be dose dependent. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Environmental Sciences; Multidisciplinary Sciences |
| 类目[WOS] | Environmental Sciences ; Multidisciplinary Sciences |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Science & Technology - Other Topics |
| 关键词[WOS] | PHYTOPLANKTIVOROUS SILVER CARP ; TROUT ONCORHYNCHUS-MYKISS ; BIOCHEMICAL-CHARACTERIZATION ; ATLANTIC SALMON ; CYPRINUS-CARPIO ; CELL-EXTRACTS ; BIGHEAD CARP ; BROWN TROUT ; IN-VIVO ; LR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000258146400001 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8046]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lei, Hehua,Xie, Ping,Chen, Jun,et al. Tissue distribution and depuration of the extracted hepatotoxic cyanotoxin microcystins in crucian carp (Carassius carassius) intraperitoneally injected at a sublethal dose[J]. THESCIENTIFICWORLDJOURNAL,2008,8(0):713-719. |
| APA | Lei, Hehua,Xie, Ping,Chen, Jun,Liang, Gaodao,Yu, Ting,&Jiang, Yan.(2008).Tissue distribution and depuration of the extracted hepatotoxic cyanotoxin microcystins in crucian carp (Carassius carassius) intraperitoneally injected at a sublethal dose.THESCIENTIFICWORLDJOURNAL,8(0),713-719. |
| MLA | Lei, Hehua,et al."Tissue distribution and depuration of the extracted hepatotoxic cyanotoxin microcystins in crucian carp (Carassius carassius) intraperitoneally injected at a sublethal dose".THESCIENTIFICWORLDJOURNAL 8.0(2008):713-719. |
入库方式： OAI收割
来源：水生生物研究所
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