Identification of a C1q family member associated with cortical granules and follicular cell apoptosis in Carassius auratus gibelio
文献类型：期刊论文
| 作者 | Mei, Jie; Chen, Bo; Yue, Huamei; Gui, Jian-Fang |
| 刊名 | MOLECULAR AND CELLULAR ENDOCRINOLOGY
 |
| 出版日期 | 2008-07-16 |
| 卷号 | 289期号:1-2页码:67-76 |
| 关键词 | C1q family follicle cortical granule apoptosis ovulation follicular cells |
| ISSN号 | 0303-7207 |
| 通讯作者 | Gui, JF, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | C1q family proteins with C1q domain have been reported in vertebrates, but their biological roles are currently unknown. In this study, a C1q-like factor, designated Carassius auratus gibelio ovary-specific C1q-like factor (CagOC1q-like), was identified as a cortical granules component. Immunofluorescence localization revealed that the C1q family member was specifically expressed in follicular epithelial cells, and associated with cortical granules in fully grown oocytes. Moreover, it was discharged to the perivitelline space and egg envelope upon fertilization. As it is the first identified C1q family member that is expressed in follicular cells that surround oocyte, CagOC1q-like was applied to detection of follicular cell apoptosis and deletion. The entire cytological process of follicular cell apoptosis and deletion was clearly seen from double visualizations of follicular cells with CagOC1q-like immunofluorescence and apoptotic follicular cells labeled by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) during oocyte maturation and ovulation. (C) 2008 Elsevier Ireland Ltd. All rights reserved. |
| 英文摘要 | C1q family proteins with C1q domain have been reported in vertebrates, but their biological roles are currently unknown. In this study, a C1q-like factor, designated Carassius auratus gibelio ovary-specific C1q-like factor (CagOC1q-like), was identified as a cortical granules component. Immunofluorescence localization revealed that the C1q family member was specifically expressed in follicular epithelial cells, and associated with cortical granules in fully grown oocytes. Moreover, it was discharged to the perivitelline space and egg envelope upon fertilization. As it is the first identified C1q family member that is expressed in follicular cells that surround oocyte, CagOC1q-like was applied to detection of follicular cell apoptosis and deletion. The entire cytological process of follicular cell apoptosis and deletion was clearly seen from double visualizations of follicular cells with CagOC1q-like immunofluorescence and apoptotic follicular cells labeled by terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) during oocyte maturation and ovulation. (C) 2008 Elsevier Ireland Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Cell Biology; Endocrinology & Metabolism |
| 类目[WOS] | Cell Biology ; Endocrinology & Metabolism |
| 研究领域[WOS] | Cell Biology ; Endocrinology & Metabolism |
| 关键词[WOS] | TUMOR-NECROSIS-FACTOR ; SILVER CRUCIAN CARP ; FACTOR-ALPHA ; RAT OVARY ; OOCYTE MATURATION ; GENE-EXPRESSION ; NEONATAL RAT ; IN-VITRO ; PROTEIN ; FISH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000257841700009 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8058]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mei, Jie,Chen, Bo,Yue, Huamei,et al. Identification of a C1q family member associated with cortical granules and follicular cell apoptosis in Carassius auratus gibelio[J]. MOLECULAR AND CELLULAR ENDOCRINOLOGY,2008,289(1-2):67-76. |
| APA | Mei, Jie,Chen, Bo,Yue, Huamei,&Gui, Jian-Fang.(2008).Identification of a C1q family member associated with cortical granules and follicular cell apoptosis in Carassius auratus gibelio.MOLECULAR AND CELLULAR ENDOCRINOLOGY,289(1-2),67-76. |
| MLA | Mei, Jie,et al."Identification of a C1q family member associated with cortical granules and follicular cell apoptosis in Carassius auratus gibelio".MOLECULAR AND CELLULAR ENDOCRINOLOGY 289.1-2(2008):67-76. |
入库方式： OAI收割
来源：水生生物研究所
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