Detection of cutaneous antibodies in excised skin explants from grass carp, Ctenopharyngodon idella (Valenciennes), immune to Scophthalmus maximus rhabdovirus
文献类型：期刊论文
| 作者 | Lue, A-J1,2; Li, Z-Q1; Zhang, Q-Y1 |
| 刊名 | JOURNAL OF FISH DISEASES
 |
| 出版日期 | 2008-08-01 |
| 卷号 | 31期号:8页码:559-565 |
| ISSN号 | 0140-7775 |
| 关键词 | Ctenopharyngodon idellus cutaneous antibodies detection grass carp Scophthalmus maximus rhabdovirus skin culture |
| 通讯作者 | Zhang, QY, Chinese Acad Sci, Inst Hydrobiol, Grad Univ, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | This study determined whether cutaneous antibodies were present in excised skin explants of grass carp, Ctenopharyngodon idella, immune to Scophthalmus maximus rhabdovirus (SMRV). Culture fluid from immune skin explants were assayed by indirect enzyme-linked immunosorbent assay (iELISA), Western blot, indirect immunofluorescent assay (IFA) and flow cytometry (FCM). iELISA showed that cutaneous antibody titres were much lower (1:12) than antiserum titres (1:1458) from intraperitoneally immunized grass carp. The phosphoprotein and matrix protein antigens of purified SMRV proteins were recognized by cutaneous antibodies from skin culture fluid using Western blot. The skin culture fluid produced staining signals in viral assembly sites and cytoplasm of SMRV-infected epithelioma papulosum cyprini (EPC) cells by IFA. FCM showed that 4.39% SMRV-infected EPC cells were detected, while non-specific reaction was seen in 2% of control cells. This is the first description of cutaneous antibodies against SMRV in grass carp. |
| 英文摘要 | This study determined whether cutaneous antibodies were present in excised skin explants of grass carp, Ctenopharyngodon idella, immune to Scophthalmus maximus rhabdovirus (SMRV). Culture fluid from immune skin explants were assayed by indirect enzyme-linked immunosorbent assay (iELISA), Western blot, indirect immunofluorescent assay (IFA) and flow cytometry (FCM). iELISA showed that cutaneous antibody titres were much lower (1:12) than antiserum titres (1:1458) from intraperitoneally immunized grass carp. The phosphoprotein and matrix protein antigens of purified SMRV proteins were recognized by cutaneous antibodies from skin culture fluid using Western blot. The skin culture fluid produced staining signals in viral assembly sites and cytoplasm of SMRV-infected epithelioma papulosum cyprini (EPC) cells by IFA. FCM showed that 4.39% SMRV-infected EPC cells were detected, while non-specific reaction was seen in 2% of control cells. This is the first description of cutaneous antibodies against SMRV in grass carp. |
| 学科主题 | Fisheries; Marine & Freshwater Biology; Veterinary Sciences |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | ICTALURUS-PUNCTATUS RAFINESQUE ; CHANNEL CATFISH ; ICHTHYOPHTHIRIUS-MULTIFILIIS ; FLAVOBACTERIUM-COLUMNARE ; MARINE FISH ; L. ; MUCOSAL ; IMMUNIZATION ; APOPTOSIS ; RESPONSES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000257493600001 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8074]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Grad Univ, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Xuzhou Normal Univ, Sch Life Sci, Xuzhou, Peoples R China |
| 推荐引用方式 GB/T 7714 | Lue, A-J,Li, Z-Q,Zhang, Q-Y. Detection of cutaneous antibodies in excised skin explants from grass carp, Ctenopharyngodon idella (Valenciennes), immune to Scophthalmus maximus rhabdovirus[J]. JOURNAL OF FISH DISEASES,2008,31(8):559-565. |
| APA | Lue, A-J,Li, Z-Q,&Zhang, Q-Y.(2008).Detection of cutaneous antibodies in excised skin explants from grass carp, Ctenopharyngodon idella (Valenciennes), immune to Scophthalmus maximus rhabdovirus.JOURNAL OF FISH DISEASES,31(8),559-565. |
| MLA | Lue, A-J,et al."Detection of cutaneous antibodies in excised skin explants from grass carp, Ctenopharyngodon idella (Valenciennes), immune to Scophthalmus maximus rhabdovirus".JOURNAL OF FISH DISEASES 31.8(2008):559-565. |
入库方式： OAI收割
来源：水生生物研究所
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