Generation of recombinant monoclonal antibodies to study structure-function of envelope protein VP28 of white spot syndrome virus from shrimp
文献类型：期刊论文
| 作者 | Wang, Yuzhen1,2; Zhang, Xiaohua1; Yuan, Li1; Xu, Tao1,2; Rao, Yu1,2; Li, Jia1,2; Dai, Heping1 |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2008-08-08 |
| 卷号 | 372期号:4页码:902-906 |
| ISSN号 | 0006-291X |
| 关键词 | WSSV VP28 phage display ScFv antibody shrimp |
| 通讯作者 | Dai, HP, Wuhan Univ, Inst Hydrobiol, Chinese Acad Sci, 7 So E Lake Rd, Wuhan 430072, Peoples R China |
| 中文摘要 | White spot syndrome virus (WSSV) is a major pathogen in shrimp aquaculture. VP28 is one of the most important envelope proteins of WSSV. In this study, a recombinant antibody library, as single-chain fragment variable (scFv) format, displayed on phage was constructed using mRNA from spleen cells of mice immunized with-full-length VP28 expressed in Escherichia coli. After several rounds of panning, six scFv antibodies specifically binding to the epitopes in the N-terminal, middle, and C-terminal regions of VP28, respectively, were isolated from the library. Using these scFv antibodies as tools, the epitopes in VP28 were located on the envelope of the virion by immuno-electron Microscopy, Neutralization assay with these antibodies in vitro suggested that these epitopes may not be the attachment site of WSSV to host cell receptor. This study provides a new way to investigate the structure and function of the envelope proteins of WSSV. (c) 2008 Published by Elsevier Inc. |
| 英文摘要 | White spot syndrome virus (WSSV) is a major pathogen in shrimp aquaculture. VP28 is one of the most important envelope proteins of WSSV. In this study, a recombinant antibody library, as single-chain fragment variable (scFv) format, displayed on phage was constructed using mRNA from spleen cells of mice immunized with-full-length VP28 expressed in Escherichia coli. After several rounds of panning, six scFv antibodies specifically binding to the epitopes in the N-terminal, middle, and C-terminal regions of VP28, respectively, were isolated from the library. Using these scFv antibodies as tools, the epitopes in VP28 were located on the envelope of the virion by immuno-electron Microscopy, Neutralization assay with these antibodies in vitro suggested that these epitopes may not be the attachment site of WSSV to host cell receptor. This study provides a new way to investigate the structure and function of the envelope proteins of WSSV. (c) 2008 Published by Elsevier Inc. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | PENAEUS-MONODON ; GENOME SEQUENCE ; WSSV ; IDENTIFICATION ; NEUTRALIZATION ; MORPHOGENESIS ; PURIFICATION ; INFECTION ; GENES ; CELLS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000257419500072 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8082]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Wuhan Univ, Inst Hydrobiol, Chinese Acad Sci, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Yuzhen,Zhang, Xiaohua,Yuan, Li,et al. Generation of recombinant monoclonal antibodies to study structure-function of envelope protein VP28 of white spot syndrome virus from shrimp[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2008,372(4):902-906. |
| APA | Wang, Yuzhen.,Zhang, Xiaohua.,Yuan, Li.,Xu, Tao.,Rao, Yu.,...&Dai, Heping.(2008).Generation of recombinant monoclonal antibodies to study structure-function of envelope protein VP28 of white spot syndrome virus from shrimp.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,372(4),902-906. |
| MLA | Wang, Yuzhen,et al."Generation of recombinant monoclonal antibodies to study structure-function of envelope protein VP28 of white spot syndrome virus from shrimp".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 372.4(2008):902-906. |
入库方式： OAI收割
来源：水生生物研究所
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