Microcystin-induced variations in transcription of GSTs in an omnivorous freshwater fish, goldfish
文献类型：期刊论文
| 作者 | Li, Guangyu; Xie, Ping; Fu, Juan; Hao, Le; Xiong, Qian; Li, Huiying |
| 刊名 | AQUATIC TOXICOLOGY
 |
| 出版日期 | 2008-06-02 |
| 卷号 | 88期号:1页码:75-80 |
| 关键词 | microcystin glutathione S-transferases real-time PCR goldfish Carassius auratus L. |
| ISSN号 | 0166-445X |
| 通讯作者 | Xie, P, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Donghu S Rd 7, Wuhan 430072, Peoples R China |
| 中文摘要 | The glutathione S-transferases are important enzymes in the microcystin-induced detoxication processes. In this experiment, we cloned the full-length cDNA of alpha, pi and theta-class-like glutathione S-transferase genes from goldfish (Carassius auratus Q. Their derived amino acid sequences were clustered with other vertebrate alpha, pi and theta-class GSTs in a phylogenetic tree and the goldfish GST sequences have the highest similarity with those from common carp and zebrafish. Goldfish were i.p. injected with microcystins extract at two doses (50 and 200 mu g kg(-1) BW MC-LReq) and the relative changes of the mRNA abundance in liver, kidney and intestine were analyzed by real-time PCR. The transcription of GST alpha was suppressed in both liver and intestine, but induced in the kidney. Decreased transcription of GST theta was detected in liver, kidney and intestine in the low-dose group. The transcription of GST pi was suppressed in liver and intestine post-injection in both dose groups. These results suggested that the transcription of GST isoforms varied in different ways within an organ and among organs of goldfish exposed to MCs. (C) 2008 Elsevier B.V. All rights reserved. |
| 英文摘要 | The glutathione S-transferases are important enzymes in the microcystin-induced detoxication processes. In this experiment, we cloned the full-length cDNA of alpha, pi and theta-class-like glutathione S-transferase genes from goldfish (Carassius auratus Q. Their derived amino acid sequences were clustered with other vertebrate alpha, pi and theta-class GSTs in a phylogenetic tree and the goldfish GST sequences have the highest similarity with those from common carp and zebrafish. Goldfish were i.p. injected with microcystins extract at two doses (50 and 200 mu g kg(-1) BW MC-LReq) and the relative changes of the mRNA abundance in liver, kidney and intestine were analyzed by real-time PCR. The transcription of GST alpha was suppressed in both liver and intestine, but induced in the kidney. Decreased transcription of GST theta was detected in liver, kidney and intestine in the low-dose group. The transcription of GST pi was suppressed in liver and intestine post-injection in both dose groups. These results suggested that the transcription of GST isoforms varied in different ways within an organ and among organs of goldfish exposed to MCs. (C) 2008 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Marine & Freshwater Biology; Toxicology |
| 类目[WOS] | Marine & Freshwater Biology ; Toxicology |
| 研究领域[WOS] | Marine & Freshwater Biology ; Toxicology |
| 关键词[WOS] | PHYTOPLANKTIVOROUS SILVER CARP ; S-TRANSFERASE GENES ; DANIO-RERIO ; RAINBOW-TROUT ; INTRAPERITONEAL INJECTION ; GLUTATHIONE TRANSFERASES ; ULTRASTRUCTURAL-CHANGES ; EXTRACTED MICROCYSTINS ; CARASSIUS-AURATUS ; CYPRINUS-CARPIO |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000257005700010 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8100]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol China, Donghu Expt Stn Lake Ecosyst, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Guangyu,Xie, Ping,Fu, Juan,et al. Microcystin-induced variations in transcription of GSTs in an omnivorous freshwater fish, goldfish[J]. AQUATIC TOXICOLOGY,2008,88(1):75-80. |
| APA | Li, Guangyu,Xie, Ping,Fu, Juan,Hao, Le,Xiong, Qian,&Li, Huiying.(2008).Microcystin-induced variations in transcription of GSTs in an omnivorous freshwater fish, goldfish.AQUATIC TOXICOLOGY,88(1),75-80. |
| MLA | Li, Guangyu,et al."Microcystin-induced variations in transcription of GSTs in an omnivorous freshwater fish, goldfish".AQUATIC TOXICOLOGY 88.1(2008):75-80. |
入库方式： OAI收割
来源：水生生物研究所
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