Inhibition of no tail (ntl) gene expression in zebrafish by external guide sequence (EGS) technique
文献类型：期刊论文
| 作者 | Pei, De-Sheng; Sun, Yong-Hua; Long, Yong; Zhu, Zuo-Yan |
| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2008-06-01 |
| 卷号 | 35期号:2页码:139-143 |
| 关键词 | external guide sequence no tail RNase P zebrafish |
| ISSN号 | 0301-4851 |
| 通讯作者 | Sun, YH, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grp Environm Genom, Wuhan 430072, Peoples R China |
| 中文摘要 | External guide sequence (EGS) technique, a branch of ribozyme strategy, can be enticed to cleave the target mRNA by forming a tRNA-like structure. In the present study, no tail gene (ntl), a key gene participating in the formation of normal tail, was used as a target for ribonuclease (RNase) P-mediated gene disruption in zebrafish in vivo. Transient expression of pH1-m3/4 ntl-EGS or pH1-3/4 ntl-EGS produced the full no tail phenotype at long-pec stage in proportion as 24 or 35%, respectively. As is expected that the full-length ntl mRNA of embryos at 50% epiboly stage decreased relative to control when injected the embryos with 3/4 EGS or m3/4 EGS RNA. Interestingly, ntl RNA transcripts, including the cleaved by EGS and the untouched, increased. Taken together, these results indicate that EGS strategy can work in zebrafish in vivo and becomes a potential tool for degradation of targeted mRNAs. |
| 英文摘要 | External guide sequence (EGS) technique, a branch of ribozyme strategy, can be enticed to cleave the target mRNA by forming a tRNA-like structure. In the present study, no tail gene (ntl), a key gene participating in the formation of normal tail, was used as a target for ribonuclease (RNase) P-mediated gene disruption in zebrafish in vivo. Transient expression of pH1-m3/4 ntl-EGS or pH1-3/4 ntl-EGS produced the full no tail phenotype at long-pec stage in proportion as 24 or 35%, respectively. As is expected that the full-length ntl mRNA of embryos at 50% epiboly stage decreased relative to control when injected the embryos with 3/4 EGS or m3/4 EGS RNA. Interestingly, ntl RNA transcripts, including the cleaved by EGS and the untouched, increased. Taken together, these results indicate that EGS strategy can work in zebrafish in vivo and becomes a potential tool for degradation of targeted mRNAs. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | RNASE-P ; RIBONUCLEASE P ; MODEL ; CLEAVAGE ; SIRNAS ; ASTHMA ; CELLS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000256082300009 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8114]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Grp Environm Genom, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pei, De-Sheng,Sun, Yong-Hua,Long, Yong,et al. Inhibition of no tail (ntl) gene expression in zebrafish by external guide sequence (EGS) technique[J]. MOLECULAR BIOLOGY REPORTS,2008,35(2):139-143. |
| APA | Pei, De-Sheng,Sun, Yong-Hua,Long, Yong,&Zhu, Zuo-Yan.(2008).Inhibition of no tail (ntl) gene expression in zebrafish by external guide sequence (EGS) technique.MOLECULAR BIOLOGY REPORTS,35(2),139-143. |
| MLA | Pei, De-Sheng,et al."Inhibition of no tail (ntl) gene expression in zebrafish by external guide sequence (EGS) technique".MOLECULAR BIOLOGY REPORTS 35.2(2008):139-143. |
入库方式： OAI收割
来源：水生生物研究所
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