Isolation, characterization and genome sequence of a birnavirus strain from flounder Paralichthys olivaceus in China
文献类型：期刊论文
| 作者 | Zhao, Zhe; Ke, Fei; Li, Zhengqiu; Gui, Jianfang; Zhang, Qiya |
| 刊名 | ARCHIVES OF VIROLOGY
 |
| 出版日期 | 2008-06-01 |
| 卷号 | 153期号:6页码:1143-1148 |
| ISSN号 | 0304-8608 |
| 关键词 | PANCREATIC NECROSIS VIRUS CELL-LINE MOLECULAR CHARACTERIZATION CLEAVAGE SITES DISEASE VIRUS VP4 PROTEASE VIRULENCE FISH RHABDOVIRUS EXPRESSION |
| 通讯作者 | Zhang, Q, Grad Univ, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Chinese Acad Sci, Wuhan 430072, Peoples R China |
| 中文摘要 | A birnavirus strain, Paralichthys olivaceus birnavirus (POBV), was isolated and characterized from cultured flounder in China, and its complete genomic sequence was subsequently determined. The virus could induce cytopathic effects (CPE) in four of seven fish cell lines and was resistant to chloroform, 5-iodo-2'-deoxyuridine, acid and alkaline pH, and heat treatment. Purified virus particles had a typical icosahedral shape, with a diameter of approximately 55-60 nm. The genomic segments A and B of POBV were 3,091 and 2,780 bp in length and shared many of the features of the members of the family Birnaviridae. Segment A contained two partially overlapping ORFs encoding a polyprotein, pVP2-VP4-VP3, and a nonstructural protein, VP5, while segment B had only one ORF encoding for the VP1, a viral RNA-dependent RNA polymerase (RdRp). This is the first report about a birnavirus strain from a new non-salmonid host in China and its complete genome sequence. |
| 英文摘要 | A birnavirus strain, Paralichthys olivaceus birnavirus (POBV), was isolated and characterized from cultured flounder in China, and its complete genomic sequence was subsequently determined. The virus could induce cytopathic effects (CPE) in four of seven fish cell lines and was resistant to chloroform, 5-iodo-2'-deoxyuridine, acid and alkaline pH, and heat treatment. Purified virus particles had a typical icosahedral shape, with a diameter of approximately 55-60 nm. The genomic segments A and B of POBV were 3,091 and 2,780 bp in length and shared many of the features of the members of the family Birnaviridae. Segment A contained two partially overlapping ORFs encoding a polyprotein, pVP2-VP4-VP3, and a nonstructural protein, VP5, while segment B had only one ORF encoding for the VP1, a viral RNA-dependent RNA polymerase (RdRp). This is the first report about a birnavirus strain from a new non-salmonid host in China and its complete genome sequence. |
| 学科主题 | Virology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Virology |
| 研究领域[WOS] | Virology |
| 关键词[WOS] | PANCREATIC NECROSIS VIRUS ; CELL-LINE ; MOLECULAR CHARACTERIZATION ; CLEAVAGE SITES ; DISEASE VIRUS ; VP4 PROTEASE ; VIRULENCE ; FISH ; RHABDOVIRUS ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000256089000016 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8128]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Grad Univ, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Chinese Acad Sci, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Zhe,Ke, Fei,Li, Zhengqiu,et al. Isolation, characterization and genome sequence of a birnavirus strain from flounder Paralichthys olivaceus in China[J]. ARCHIVES OF VIROLOGY,2008,153(6):1143-1148. |
| APA | Zhao, Zhe,Ke, Fei,Li, Zhengqiu,Gui, Jianfang,&Zhang, Qiya.(2008).Isolation, characterization and genome sequence of a birnavirus strain from flounder Paralichthys olivaceus in China.ARCHIVES OF VIROLOGY,153(6),1143-1148. |
| MLA | Zhao, Zhe,et al."Isolation, characterization and genome sequence of a birnavirus strain from flounder Paralichthys olivaceus in China".ARCHIVES OF VIROLOGY 153.6(2008):1143-1148. |
入库方式： OAI收割
来源：水生生物研究所
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