Avian influenza virus infection induces differential expression of genes in chicken kidney
文献类型：期刊论文
| 作者 | Zhang, Wanpo1; Li, Huiying2; Cheng, Guofu1; Hu, Sishun1; Li, Zili1; Bi, Dingren1 |
| 刊名 | RESEARCH IN VETERINARY SCIENCE
 |
| 出版日期 | 2008-06-01 |
| 卷号 | 84期号:3页码:374-381 |
| 关键词 | avian influenza virus (AIV) chicken suppression subtractive hybridization (SSH) gene differential expression |
| ISSN号 | 0034-5288 |
| 通讯作者 | Bi, DG, Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China |
| 中文摘要 | The pathogenic process of highly pathogenic avian influenza virus (HPAIV) infection is poorly understood. To explore the differential expression of kidney genes as a result of HPAIV infection, two cDNA libraries were constructed from uninfected and infected kidneys by suppression subtractive hybridization (SSH). Fifteen genes including IFN-stimulated genes (ISG12), lymphocyte antigen 6 complex locus E gene (LY6E), matrix Gla protein gene (MGP), lysozyme gene, haemopoiesis related membrane protein I gene, KIAA1259, MGC68696, G6pe-prov protein gene (G6PC), MGC4504, alcohol dehydrogenase gene (ADH), glutathione S-transferase gene (GST), sodium-dependent high-affinity dicarboxylate transporter gene (SDCT), Synaptotagmin XV (SytXV) and two novel genes were found significantly up-regulated or dramatically suppressed. Differential expression of these genes was further identified by Northern blot. Functional analysis indicated that the regulation of their expression might contribute to the pathogenic process of HPAIV infection. In contrast, the increased expression of three IFN-stimulated genes named ISG12, LY6E, and haemopoiesis related membrane protein 1 gene might reflect host defense responses. Further study showed that ISG12 protein failed to directly interact with NS1 protein of HPAIV which expressed simultaneously in the organs where HPAIV replication occurred, by use of BacterioMatch two-hybrid system. Therefore, our findings may provide new insights into understanding the molecular mechanism underlying the pathophysiological process of HPAIV infection in chicken. (c) 2007 Elsevier Ltd. All rights reserved. |
| 英文摘要 | The pathogenic process of highly pathogenic avian influenza virus (HPAIV) infection is poorly understood. To explore the differential expression of kidney genes as a result of HPAIV infection, two cDNA libraries were constructed from uninfected and infected kidneys by suppression subtractive hybridization (SSH). Fifteen genes including IFN-stimulated genes (ISG12), lymphocyte antigen 6 complex locus E gene (LY6E), matrix Gla protein gene (MGP), lysozyme gene, haemopoiesis related membrane protein I gene, KIAA1259, MGC68696, G6pe-prov protein gene (G6PC), MGC4504, alcohol dehydrogenase gene (ADH), glutathione S-transferase gene (GST), sodium-dependent high-affinity dicarboxylate transporter gene (SDCT), Synaptotagmin XV (SytXV) and two novel genes were found significantly up-regulated or dramatically suppressed. Differential expression of these genes was further identified by Northern blot. Functional analysis indicated that the regulation of their expression might contribute to the pathogenic process of HPAIV infection. In contrast, the increased expression of three IFN-stimulated genes named ISG12, LY6E, and haemopoiesis related membrane protein 1 gene might reflect host defense responses. Further study showed that ISG12 protein failed to directly interact with NS1 protein of HPAIV which expressed simultaneously in the organs where HPAIV replication occurred, by use of BacterioMatch two-hybrid system. Therefore, our findings may provide new insights into understanding the molecular mechanism underlying the pathophysiological process of HPAIV infection in chicken. (c) 2007 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Veterinary Sciences |
| 类目[WOS] | Veterinary Sciences |
| 研究领域[WOS] | Veterinary Sciences |
| 关键词[WOS] | SUPPRESSION SUBTRACTIVE HYBRIDIZATION ; A VIRUSES ; PROTEIN ; IDENTIFICATION ; H5N1 ; ISG12 ; MICE ; GLUTATHIONE ; PROFILE ; CLONING |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000255832900010 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8132]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Huazhong Agr Univ, Coll Vet Med, State Key Lab Agr Microbiol, Wuhan 430070, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Wanpo,Li, Huiying,Cheng, Guofu,et al. Avian influenza virus infection induces differential expression of genes in chicken kidney[J]. RESEARCH IN VETERINARY SCIENCE,2008,84(3):374-381. |
| APA | Zhang, Wanpo,Li, Huiying,Cheng, Guofu,Hu, Sishun,Li, Zili,&Bi, Dingren.(2008).Avian influenza virus infection induces differential expression of genes in chicken kidney.RESEARCH IN VETERINARY SCIENCE,84(3),374-381. |
| MLA | Zhang, Wanpo,et al."Avian influenza virus infection induces differential expression of genes in chicken kidney".RESEARCH IN VETERINARY SCIENCE 84.3(2008):374-381. |
入库方式： OAI收割
来源：水生生物研究所
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