The effective expression of xylanase gene in Candida utilis by 18S rDNA targeted homologous recombination in pGLR9K
文献类型：期刊论文
| 作者 | Wang Wei ; Yang Hong-Lan ; Bao HuiFang ; Zhang Daoyuan ; Shan Qi-mu-ge ; Wood ; Andrew J. |
| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2010 |
| 卷号 | 37期号:6页码:2615-2620 |
| 关键词 | RIBOSOMAL DNA YEAST INTEGRATION TRANSFORMATION PROTEIN TOBACCO VECTOR MARKER |
| ISSN号 | 0301-4851 |
| 通讯作者 | Wang, W, Xinjiang Acad Agr Sci, Inst Microbiol, Urumqi 830091, Peoples R China |
| 中文摘要 | In order to test whether 18S rDNA can influence positively xylanase gene effective expression in the yeast of Candida utilis, a targeting vector pGLR9K-XA was constructed by adding an interested gene xynA from Streptomyces olivaceoviridis into the vector pGLR9K which is constructed by ourselves. pGLR9K contains the 18S rDNA, GAP promoter and CYH resistance gene sequence, all of which is from C. utilis. Then the vector pGLR9K-XA was transformed into C. utilis. To test the vector and transformed system, PCR, Southern blot and DNS methods were used. The results showed that xylanase gene can be detected in the chromosome DNA of recombinant C. utilis and the enzyme activity of xylanase is up to 60 IU ml(-1) in the study. It is suggested that this system can be used to express exogenous genes in C. utilis as a bioreactors. This is the first report that xylanase gene was expressed in C. utilis. |
| 学科主题 | 生物化学与分子生物学 |
| 收录类别 | SCI |
| 公开日期 | 2011-08-19 |
| 源URL | [http://ir.xjlas.org/handle/365004/9940]  |
| 专题 | 新疆生态与地理研究所_中国科学院新疆生态与地理研究所(2010年以前数据) |
| 推荐引用方式 GB/T 7714 | Wang Wei,Yang Hong-Lan,Bao HuiFang,et al. The effective expression of xylanase gene in Candida utilis by 18S rDNA targeted homologous recombination in pGLR9K[J]. MOLECULAR BIOLOGY REPORTS,2010,37(6):2615-2620. |
| APA | Wang Wei.,Yang Hong-Lan.,Bao HuiFang.,Zhang Daoyuan.,Shan Qi-mu-ge.,...&Andrew J..(2010).The effective expression of xylanase gene in Candida utilis by 18S rDNA targeted homologous recombination in pGLR9K.MOLECULAR BIOLOGY REPORTS,37(6),2615-2620. |
| MLA | Wang Wei,et al."The effective expression of xylanase gene in Candida utilis by 18S rDNA targeted homologous recombination in pGLR9K".MOLECULAR BIOLOGY REPORTS 37.6(2010):2615-2620. |
入库方式： OAI收割
来源：新疆生态与地理研究所
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