Deletion of yeast CWP genes enhances cell permeability to genotoxic agents
文献类型：期刊论文
| 作者 | Zhang, Min1; Liang, Yuping2; Zhang, Xiaohua1; Xu, Ying1; Dai, Heping1; Xiao, Wei1,2 |
| 刊名 | TOXICOLOGICAL SCIENCES
 |
| 出版日期 | 2008-05-01 |
| 卷号 | 103期号:1页码:68-76 |
| 关键词 | RNR3-lacZ yeast cell wall mannoprotein genotoxicity test sensitivity permeability |
| ISSN号 | 1096-6080 |
| 通讯作者 | Dai, HP, Chinese Acad Sci, Inst Hydrol, 7 Donghu Rd S, Wuhan 430072, Peoples R China |
| 中文摘要 | We have previously reported the development of a novel genotoxic testing system based on the transcriptional response of the yeast RNR3-lacZ reporter gene to DNA damage. This system appears to be more sensitive than other similar tests in microorganisms, and is comparable with the Ames test. In an effort to further enhance detection sensitivity, we examined the effects of altering major cell wall components on cell permeability and subsequent RNR3-lacZ sensitivity to genotoxic agents. Although inactivation of single CWP genes encoding cell wall mannoproteins had little effect, the simultaneous inactivation of both CWP1 and CWP2 had profound effects on the cell wall structure and permeability. Consequently, the RNR3-lacZ detection sensitivity is markedly enhanced, especially to high molecular weight compounds such as 4-nitroquinoline-N-oxide (> sevenfold) and phleomycin (> 13-fold). In contrast, deletion of genes encoding representative membrane components or membrane transporters had minor effects on cell permeability. We conclude that the yeast cell wall mannoproteins constitute the major barrier to environmental genotoxic agents and that their removal will significantly enhance the sensitivity of RNR-lacZ as well as other yeast-based genotoxic tests. |
| 英文摘要 | We have previously reported the development of a novel genotoxic testing system based on the transcriptional response of the yeast RNR3-lacZ reporter gene to DNA damage. This system appears to be more sensitive than other similar tests in microorganisms, and is comparable with the Ames test. In an effort to further enhance detection sensitivity, we examined the effects of altering major cell wall components on cell permeability and subsequent RNR3-lacZ sensitivity to genotoxic agents. Although inactivation of single CWP genes encoding cell wall mannoproteins had little effect, the simultaneous inactivation of both CWP1 and CWP2 had profound effects on the cell wall structure and permeability. Consequently, the RNR3-lacZ detection sensitivity is markedly enhanced, especially to high molecular weight compounds such as 4-nitroquinoline-N-oxide (> sevenfold) and phleomycin (> 13-fold). In contrast, deletion of genes encoding representative membrane components or membrane transporters had minor effects on cell permeability. We conclude that the yeast cell wall mannoproteins constitute the major barrier to environmental genotoxic agents and that their removal will significantly enhance the sensitivity of RNR-lacZ as well as other yeast-based genotoxic tests. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Toxicology |
| 类目[WOS] | Toxicology |
| 研究领域[WOS] | Toxicology |
| 关键词[WOS] | PLEIOTROPIC DRUG-RESISTANCE ; SACCHAROMYCES-CEREVISIAE ; MULTIDRUG-RESISTANCE ; CALCOFLUOR WHITE ; TESTING SYSTEM ; WALL POROSITY ; CONGO RED ; MEMBRANE ; MANNOPROTEINS ; SENSITIVITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000254955500008 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8160]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrol, Wuhan 430072, Peoples R China 2.Univ Saskatchewan, Dept Microbiol & Immunol, Saskatoon, SK S7N 5E5, Canada |
| 推荐引用方式 GB/T 7714 | Zhang, Min,Liang, Yuping,Zhang, Xiaohua,et al. Deletion of yeast CWP genes enhances cell permeability to genotoxic agents[J]. TOXICOLOGICAL SCIENCES,2008,103(1):68-76. |
| APA | Zhang, Min,Liang, Yuping,Zhang, Xiaohua,Xu, Ying,Dai, Heping,&Xiao, Wei.(2008).Deletion of yeast CWP genes enhances cell permeability to genotoxic agents.TOXICOLOGICAL SCIENCES,103(1),68-76. |
| MLA | Zhang, Min,et al."Deletion of yeast CWP genes enhances cell permeability to genotoxic agents".TOXICOLOGICAL SCIENCES 103.1(2008):68-76. |
入库方式： OAI收割
来源：水生生物研究所
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