Proline accumulation inside symbiosomes of faba bean nodules under salt stress
文献类型：期刊论文
| 作者 | Trinchant ; JC ; Yang ; YS ; Rigaud ; J |
| 刊名 | PHYSIOLOGIA PLANTARUM
 |
| 出版日期 | 1998 |
| 卷号 | 104期号:1页码:38-49 |
| 关键词 | FIXATION C2H2 REDUCTION SODIUM-CHLORIDE STRESS SOYBEAN NODULES NITROGEN-FIXATION ACETYLENE-REDUCTION AMINO-ACID DELTA-1-PYRROLINE-5-CARBOXYLATE REDUCTASE PYRROLINE-5-CARBOXYLATE REDUCTASE CARBON METABOLISM FREE BACTEROIDS |
| ISSN号 | 0031-9317 |
| 通讯作者 | Trinchant, JC, Univ Nice Sophia Antipolis, Lab Biol Vegetale & Microbiol, CNRS, ERS 0590, Parc Valrose, F-06108 Nice 2, France |
| 中文摘要 | A two-week salt treatment (NaCl, 100 mM) induced a 50% inhibition of acetylene reduction activity (ARA) of faba bean (Vicia faba L. var. minor cv. Soravi) nodules, associated with a large increase in the nodule pool of amino acids. The concentration of proline in the different nodule compartments was determined after calculating their respective volumes from their areas on electron micrographs. The proline concentration exhibited a large increase, especially in the cytosol where its amount was 8-fold enhanced under salt stress, whereas the low proline content of bacteroids was less affected. Increase of proline concentration in faba bean nodules subjected to salt stress was correlated with an enhancement of the cytosolic Delta(1)-pyrroline-5-carboxylate synthetase (EC 2.7.2.11 + EC 1.2.1.41; P5CS) activity. Experiments with purified symbiosome preparations showed that the greatest proline content occurred in the peribacteroid space (PBS), where proline was the most abundant amino acid, with a concentration reaching 15.3 mM under salt stress. Proline accumulation in the PBS resulted both from a diffusive transport from the host cell to the symbiosomes through the peribacteroid membrane (PBM) and from the very low rats of uptake by faba bean bacteroids. This accumulation could be partly responsible for the 1.7-fold enlargement of the symbiosome volume observed in salt-stressed nodules. In incubations of bacteroids, isolated from salt-stressed or unstressed plants and supplied with O-2 by purified oxyleghemoglobin, addition of proline stimulated neither O-2 consumption nor ARA. These results were consistent with proline playing a role as osmoticum, rather than energy source for bacteroid N-2 fixation in amide-exporting legumes such as faba bean. |
| 学科主题 | 植物生理学 |
| 收录类别 | SCI |
| 公开日期 | 2011-08-19 |
| 源URL | [http://ir.xjlas.org/handle/365004/10658]  |
| 专题 | 新疆生态与地理研究所_中国科学院新疆生态与地理研究所(2010年以前数据) |
| 推荐引用方式 GB/T 7714 | Trinchant,JC,Yang,et al. Proline accumulation inside symbiosomes of faba bean nodules under salt stress[J]. PHYSIOLOGIA PLANTARUM,1998,104(1):38-49. |
| APA | Trinchant,JC,Yang,YS,Rigaud,&J.(1998).Proline accumulation inside symbiosomes of faba bean nodules under salt stress.PHYSIOLOGIA PLANTARUM,104(1),38-49. |
| MLA | Trinchant,et al."Proline accumulation inside symbiosomes of faba bean nodules under salt stress".PHYSIOLOGIA PLANTARUM 104.1(1998):38-49. |
入库方式： OAI收割
来源：新疆生态与地理研究所
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