Toll-like receptor 3 regulates Mx expression in rare minnow Gobiocypris rarus after viral infection
文献类型：期刊论文
| 作者 | Su, Jianguo1,2; Zhu, Zuoyan1; Wang, Yaping1; Zou, Jun3; Hu, Wei1 |
| 刊名 | IMMUNOGENETICS
 |
| 出版日期 | 2008-04-01 |
| 卷号 | 60期号:3-4页码:195-205 |
| 关键词 | Gobiocypris rarus TLR3 Mx transgenic fish RNAi |
| ISSN号 | 0093-7711 |
| 通讯作者 | Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | Toll-like receptor 3 (TLR3) plays a key role in activating immune responses during viral infection. To study the genes involved in the regulatory function of TLR3 in the rare minnow Gobiocypris rarus after viral infection, a full-length cDNA of TLR3 (GrTLR3) with a splice variant (GrTLR3s) was identified by homologous cloning and RACE techniques. The antiviral effector molecule Mx gene was cloned and partially sequenced. The mRNA expression levels of GrTLR3, GrTLR3s, and Mx were studied in different tissues before and after virus infection by real-time quantitative RT-PCR. The transcripts of all three genes in liver were significantly increased following GCRV infection (P<0.05). The mRNA levels in liver were upregulated at 24 h post-injection for GrTLR3 and GrTLR3s, and at 12 h for Mx. The upregulated expression levels were several folds for GrTLR3s, tens of folds for GrTLR3, and hundreds of folds for Mx. By semi-quantitative RT-PCR, GrTLR3 and Mx expressed at all the developmental stages, whereas GrTLR3s could only be detected at later developmental stages. Using RNAi and transgenic techniques, GrTLR3 mediated Mx expression but GrTLR3s did not. The time-dependent upregulation of receptor and effector, and the Mx over-expression dependent on TLR3, indicated that GrTLR3 regulated Mx expression in viral infection through a configuration change in rare minnow, and its splice variant did not contribute to the process. |
| 英文摘要 | Toll-like receptor 3 (TLR3) plays a key role in activating immune responses during viral infection. To study the genes involved in the regulatory function of TLR3 in the rare minnow Gobiocypris rarus after viral infection, a full-length cDNA of TLR3 (GrTLR3) with a splice variant (GrTLR3s) was identified by homologous cloning and RACE techniques. The antiviral effector molecule Mx gene was cloned and partially sequenced. The mRNA expression levels of GrTLR3, GrTLR3s, and Mx were studied in different tissues before and after virus infection by real-time quantitative RT-PCR. The transcripts of all three genes in liver were significantly increased following GCRV infection (P<0.05). The mRNA levels in liver were upregulated at 24 h post-injection for GrTLR3 and GrTLR3s, and at 12 h for Mx. The upregulated expression levels were several folds for GrTLR3s, tens of folds for GrTLR3, and hundreds of folds for Mx. By semi-quantitative RT-PCR, GrTLR3 and Mx expressed at all the developmental stages, whereas GrTLR3s could only be detected at later developmental stages. Using RNAi and transgenic techniques, GrTLR3 mediated Mx expression but GrTLR3s did not. The time-dependent upregulation of receptor and effector, and the Mx over-expression dependent on TLR3, indicated that GrTLR3 regulated Mx expression in viral infection through a configuration change in rare minnow, and its splice variant did not contribute to the process. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Genetics & Heredity; Immunology |
| 类目[WOS] | Genetics & Heredity ; Immunology |
| 研究领域[WOS] | Genetics & Heredity ; Immunology |
| 关键词[WOS] | DOUBLE-STRANDED-RNA ; MESSENGER-RNA ; ADAPTER MOLECULE ; GENE-EXPRESSION ; DANIO-RERIO ; TLR3 ; RECOGNITION ; PROTEIN ; INDUCTION ; IMMUNITY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000255113300008 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8170]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.NW & A&F Univ, Yangling 712100, Peoples R China 3.Univ Aberdeen, Sch Biol Sci, Aberdeen AB24 2TZ, Scotland |
| 推荐引用方式 GB/T 7714 | Su, Jianguo,Zhu, Zuoyan,Wang, Yaping,et al. Toll-like receptor 3 regulates Mx expression in rare minnow Gobiocypris rarus after viral infection[J]. IMMUNOGENETICS,2008,60(3-4):195-205. |
| APA | Su, Jianguo,Zhu, Zuoyan,Wang, Yaping,Zou, Jun,&Hu, Wei.(2008).Toll-like receptor 3 regulates Mx expression in rare minnow Gobiocypris rarus after viral infection.IMMUNOGENETICS,60(3-4),195-205. |
| MLA | Su, Jianguo,et al."Toll-like receptor 3 regulates Mx expression in rare minnow Gobiocypris rarus after viral infection".IMMUNOGENETICS 60.3-4(2008):195-205. |
入库方式： OAI收割
来源：水生生物研究所
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