The biological effects of rainbow trout (Oncorhynchus mykiss) recombinant interleukin-8
文献类型：期刊论文
| 作者 | Harun, Nor Omaima1; Zou, Jun1; Zhang, Yang-An1,2; Nie, Pin2; Secombes, Chris J.1 |
| 刊名 | DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
 |
| 出版日期 | 2008 |
| 卷号 | 32期号:6页码:673-681 |
| 关键词 | rainbow trout recombinant protein interleukin-8 biological activity migration respiratory burst |
| ISSN号 | 0145-305X |
| 通讯作者 | Secombes, CJ, Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Tillydrone Ave, Aberdeen AB24 2TZ, Scotland |
| 中文摘要 | In this report, recombinant interieukin-8 (rIL-8) was produced and its activity tested for the first time in fish. The rainbow trout rIL-8 was produced in Escherichia coli and purified using a 6xHis tag at the N-terminus. The rIL-8 induced a dose-dependent migration of head kidney leukocytes at concentrations from 0.1 to 10 ng/ml, with a peak response at 1 ng/ml. Trout rIL-8 also had a significant effect on superoxide production by head kidney cells, with maximal, activity at 0.1 and 1 ng/ml. When injected intraperitoneally into trout, rIL-8 had a clear effect on total leukocyte number in the peritoneal cavity, with increasing doses (up to 5 mu g) eliciting more cells. Of three leukocyte types distinguished, neutrophils were the dominant cell type, especially at higher rIL-8 concentrations. In contrast, the proportion of macrophages and lymphocytes decreased with rIL-8 administration, suggesting that they were not attracted at the same rate as neutrophils. (C) 2007 Elsevier Ltd. All rights reserved. |
| 英文摘要 | In this report, recombinant interieukin-8 (rIL-8) was produced and its activity tested for the first time in fish. The rainbow trout rIL-8 was produced in Escherichia coli and purified using a 6xHis tag at the N-terminus. The rIL-8 induced a dose-dependent migration of head kidney leukocytes at concentrations from 0.1 to 10 ng/ml, with a peak response at 1 ng/ml. Trout rIL-8 also had a significant effect on superoxide production by head kidney cells, with maximal, activity at 0.1 and 1 ng/ml. When injected intraperitoneally into trout, rIL-8 had a clear effect on total leukocyte number in the peritoneal cavity, with increasing doses (up to 5 mu g) eliciting more cells. Of three leukocyte types distinguished, neutrophils were the dominant cell type, especially at higher rIL-8 concentrations. In contrast, the proportion of macrophages and lymphocytes decreased with rIL-8 administration, suggesting that they were not attracted at the same rate as neutrophils. (C) 2007 Elsevier Ltd. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Immunology; Zoology |
| 类目[WOS] | Immunology ; Zoology |
| 研究领域[WOS] | Immunology ; Zoology |
| 关键词[WOS] | PERITONEAL-CAVITY ; FUNCTIONAL-CHARACTERIZATION ; HOST DEFENSE ; IN-VITRO ; NEUTROPHIL ; CLONING ; CHEMOTAXIS ; LEUKOCYTES ; MIGRATION ; CYTOKINE |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000254781600009 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8186]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Univ Aberdeen, Sch Biol Sci, Scottish Fish Immunol Res Ctr, Aberdeen AB24 2TZ, Scotland 2.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Harun, Nor Omaima,Zou, Jun,Zhang, Yang-An,et al. The biological effects of rainbow trout (Oncorhynchus mykiss) recombinant interleukin-8[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2008,32(6):673-681. |
| APA | Harun, Nor Omaima,Zou, Jun,Zhang, Yang-An,Nie, Pin,&Secombes, Chris J..(2008).The biological effects of rainbow trout (Oncorhynchus mykiss) recombinant interleukin-8.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,32(6),673-681. |
| MLA | Harun, Nor Omaima,et al."The biological effects of rainbow trout (Oncorhynchus mykiss) recombinant interleukin-8".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 32.6(2008):673-681. |
入库方式： OAI收割
来源：水生生物研究所
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