Identification and characterization of a novel gene differentially expressed in zebrafish cross-subfamily cloned embryos
文献类型：期刊论文
| 作者 | Pei, De-Sheng1,2; Sun, Yong-Hua1; Chen, Chun-Hong1,3; Chen, Shang-Ping1; Wang, Ya-Ping1; Hu, Wei1; Zhu, Zuo-Yan1 |
| 刊名 | BMC DEVELOPMENTAL BIOLOGY
 |
| 出版日期 | 2008-03-18 |
| 卷号 | 8页码:- |
| ISSN号 | 1471-213X |
| 关键词 | NUCLEAR TRANSFER GOBIOCYPRIS-RARUS REACTIVATION PREDICTION CLONING SIGNAL FISH |
| 通讯作者 | Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | m Background: Cross-species nuclear transfer has been shown to be a potent approach to retain the genetic viability of a certain species near extinction. However, most embryos produced by cross-species nuclear transfer were compromised because that they were unable to develop to later stages. Gene expression analysis of cross-species cloned embryos will yield new insights into the regulatory mechanisms involved in cross-species nuclear transfer and embryonic development. Results: A novel gene, K31, was identified as an up-regulated gene in fish cross-subfamily cloned embryos using SSH approach and RACE method. K31 complete cDNA sequence is 1106 base pairs (bp) in length, with a 342 bp open reading frame (ORF) encoding a putative protein of 113 amino acids (aa). Comparative analysis revealed no homologous known gene in zebrafish and other species database. K31 protein contains a putative transmembrane helix and five putative phosphorylation sites but without a signal peptide. Expression pattern analysis by real time RT-PCR and whole-mount in situ hybridization (WISH) shows that it has the characteristics of constitutively expressed gene. Sub-cellular localization assay shows that K31 protein can not penetrate the nuclei. Interestingly, over-expression of K31 gene can cause lethality in the epithelioma papulosum cyprinid (EPC) cells in cell culture, which gave hint to the inefficient reprogramming events occurred in cloned embryos. Conclusion: Taken together, our findings indicated that K31 gene is a novel gene differentially expressed in fish cross-subfamily cloned embryos and over-expression of K31 gene can cause lethality of cultured fish cells. To our knowledge, this is the first report on the determination of novel genes involved in nucleo-cytoplasmic interaction of fish cross-subfamily cloned embryos. |
| 英文摘要 | m Background: Cross-species nuclear transfer has been shown to be a potent approach to retain the genetic viability of a certain species near extinction. However, most embryos produced by cross-species nuclear transfer were compromised because that they were unable to develop to later stages. Gene expression analysis of cross-species cloned embryos will yield new insights into the regulatory mechanisms involved in cross-species nuclear transfer and embryonic development. |
| 学科主题 | Developmental Biology |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Developmental Biology |
| 研究领域[WOS] | Developmental Biology |
| 关键词[WOS] | NUCLEAR TRANSFER ; GOBIOCYPRIS-RARUS ; REACTIVATION ; PREDICTION ; CLONING ; SIGNAL ; FISH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000254616600001 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8198]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Grp Environm Genom, Wuhan 430072, Peoples R China 3.Wuhan Univ, Coll Life Sci, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pei, De-Sheng,Sun, Yong-Hua,Chen, Chun-Hong,et al. Identification and characterization of a novel gene differentially expressed in zebrafish cross-subfamily cloned embryos[J]. BMC DEVELOPMENTAL BIOLOGY,2008,8:-. |
| APA | Pei, De-Sheng.,Sun, Yong-Hua.,Chen, Chun-Hong.,Chen, Shang-Ping.,Wang, Ya-Ping.,...&Zhu, Zuo-Yan.(2008).Identification and characterization of a novel gene differentially expressed in zebrafish cross-subfamily cloned embryos.BMC DEVELOPMENTAL BIOLOGY,8,-. |
| MLA | Pei, De-Sheng,et al."Identification and characterization of a novel gene differentially expressed in zebrafish cross-subfamily cloned embryos".BMC DEVELOPMENTAL BIOLOGY 8(2008):-. |
入库方式： OAI收割
来源：水生生物研究所
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