Acute effects of microcystins on the transcription of antioxidant enzyme genes in crucian carp Carassius auratus
文献类型：期刊论文
| 作者 | Sun, Yuhua1; Tang, Rong1; Li, Dapeng1; Zhang, Xuzhen1; Fu, Juan2; Xie, Ping1,2 |
| 刊名 | ENVIRONMENTAL TOXICOLOGY
 |
| 出版日期 | 2008-04-01 |
| 卷号 | 23期号:2页码:145-152 |
| 关键词 | microcystins mRNA expression antioxidant enzymes crucian carp (Carassius auratus) |
| ISSN号 | 1520-4081 |
| 通讯作者 | Xie, P, Huazhong Agr Univ, Key Lab Anim Dev Genet & Reprod, Educ Minist, Coll Fisheries, Wuhan 430070, Peoples R China |
| 中文摘要 | Recent evidences suggested that oxidative stress may play a significant role in the pathogenesis of MCs toxicity. In the present study, the acute effects of microcystins on the transcription of antioxidant enzyme genes were investigated in liver of crucian carp i.p.-injected with 50 mu g MC-LReq per kg body weight (BW). We reported the cDNA sequences for four kinds of antioxidant enzyme (GSH-PX, CAT, Cu/Zn SOD, and GR) genes, and evaluated the oxidant stress induced by MCs through analyzing the transcription abundance of antioxidant enzyme genes using real-time PCR method. The time-dependent change of relative transcription abundance and expression of the antioxiclant enzyme genes were determined at 1, 3, 12, 24, and 48 h. The transcription abundance varied among antioxiclant enzymes, with GSH-PX and GR down-regulation, and CAT and SOD significantly upregulation. Based on these data, we tentatively concluded that the oxidant stress was induced by MCs, and caused the different response of the antioxiclant enzyme genes. (c) 2008 Wiley Periodicals, Inc. |
| 英文摘要 | Recent evidences suggested that oxidative stress may play a significant role in the pathogenesis of MCs toxicity. In the present study, the acute effects of microcystins on the transcription of antioxidant enzyme genes were investigated in liver of crucian carp i.p.-injected with 50 mu g MC-LReq per kg body weight (BW). We reported the cDNA sequences for four kinds of antioxidant enzyme (GSH-PX, CAT, Cu/Zn SOD, and GR) genes, and evaluated the oxidant stress induced by MCs through analyzing the transcription abundance of antioxidant enzyme genes using real-time PCR method. The time-dependent change of relative transcription abundance and expression of the antioxiclant enzyme genes were determined at 1, 3, 12, 24, and 48 h. The transcription abundance varied among antioxiclant enzymes, with GSH-PX and GR down-regulation, and CAT and SOD significantly upregulation. Based on these data, we tentatively concluded that the oxidant stress was induced by MCs, and caused the different response of the antioxiclant enzyme genes. (c) 2008 Wiley Periodicals, Inc. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences |
| 学科主题 | Environmental Sciences; Toxicology; Water Resources |
| 类目[WOS] | Environmental Sciences ; Toxicology ; Water Resources |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Toxicology ; Water Resources |
| 关键词[WOS] | FISH OREOCHROMIS SP. ; INDUCED DNA-DAMAGE ; OXIDATIVE STRESS ; IN-VIVO ; LIPID-PEROXIDATION ; DANIO-RERIO ; LR ; LIVER ; CYANOBACTERIA ; HEPATOCYTES |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000254495400001 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8214]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Huazhong Agr Univ, Key Lab Anim Dev Genet & Reprod, Educ Minist, Coll Fisheries, Wuhan 430070, Peoples R China 2.Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Yuhua,Tang, Rong,Li, Dapeng,et al. Acute effects of microcystins on the transcription of antioxidant enzyme genes in crucian carp Carassius auratus[J]. ENVIRONMENTAL TOXICOLOGY,2008,23(2):145-152. |
| APA | Sun, Yuhua,Tang, Rong,Li, Dapeng,Zhang, Xuzhen,Fu, Juan,&Xie, Ping.(2008).Acute effects of microcystins on the transcription of antioxidant enzyme genes in crucian carp Carassius auratus.ENVIRONMENTAL TOXICOLOGY,23(2),145-152. |
| MLA | Sun, Yuhua,et al."Acute effects of microcystins on the transcription of antioxidant enzyme genes in crucian carp Carassius auratus".ENVIRONMENTAL TOXICOLOGY 23.2(2008):145-152. |
入库方式： OAI收割
来源：水生生物研究所
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