Genomic sequence of mandarin fish rhabdovirus with an unusual small non-transcriptional ORF
文献类型：期刊论文
| 作者 | Tao, Jian-Jun; Zhou, Guang-Zhou; Gui, Han-Farig; Zhang, Qi-Ya |
| 刊名 | VIRUS RESEARCH
 |
| 出版日期 | 2008-03-01 |
| 卷号 | 132期号:1-2页码:86-96 |
| 关键词 | genomic sequence rhabdovirus mandarin fish non-transcriptional ORF |
| ISSN号 | 0168-1702 |
| 通讯作者 | Zhang, QY, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | The complete genome of mandarin fish Siniperca chuatsi rhabdovirus (SCRV) was cloned and sequenced. It comprises 11,545 nucleotides and contains five genes encoding the nucleoprotein N, the phosphoprotein P, the matrix protein M, the glycoprotein G, and the RNA-dependent RNA polymerase protein L. At the 3' and 5' termini of SCRV genome, leader and trailer sequences show inverse complementarity. The N, P, M and G proteins share the highest sequence identities (ranging from 14.8 to 41.5%) with the respective proteins of rhabdovirus 903/87, the L protein has the highest identity with those of vesiculoviruses, especially with Chandipura virus (44.7%). Phylogenetic analysis of L proteins showed that SCRV clustered with spring vireamia of carp virus (SVCV) and was most closely related to viruses in the genus Vesiculovirus. In addition, an overlapping open reading frame (ORF) predicted to encode a protein similar to vesicular stomatitis virus C protein is present within the P gene of SCRV. Furthermore, an unoverlapping small ORF downstream of M ORF within M gene is predicted (tentatively called orf4). Therefore, the genomic organization of SCRV can be proposed as 3' leader-N-P/C-M-(orf4)-G-L-trailer 5'. Orf4 transcription or translation products could not be detected by northern or Western blot, respectively, though one similar mRNA band to M mRNA was found. This is the first report on one small unoverlapping ORF in M gene of a fish rhabdovirus. (c) 2007 Elsevier B.V. All rights reserved. |
| 英文摘要 | The complete genome of mandarin fish Siniperca chuatsi rhabdovirus (SCRV) was cloned and sequenced. It comprises 11,545 nucleotides and contains five genes encoding the nucleoprotein N, the phosphoprotein P, the matrix protein M, the glycoprotein G, and the RNA-dependent RNA polymerase protein L. At the 3' and 5' termini of SCRV genome, leader and trailer sequences show inverse complementarity. The N, P, M and G proteins share the highest sequence identities (ranging from 14.8 to 41.5%) with the respective proteins of rhabdovirus 903/87, the L protein has the highest identity with those of vesiculoviruses, especially with Chandipura virus (44.7%). Phylogenetic analysis of L proteins showed that SCRV clustered with spring vireamia of carp virus (SVCV) and was most closely related to viruses in the genus Vesiculovirus. In addition, an overlapping open reading frame (ORF) predicted to encode a protein similar to vesicular stomatitis virus C protein is present within the P gene of SCRV. Furthermore, an unoverlapping small ORF downstream of M ORF within M gene is predicted (tentatively called orf4). Therefore, the genomic organization of SCRV can be proposed as 3' leader-N-P/C-M-(orf4)-G-L-trailer 5'. Orf4 transcription or translation products could not be detected by northern or Western blot, respectively, though one similar mRNA band to M mRNA was found. This is the first report on one small unoverlapping ORF in M gene of a fish rhabdovirus. (c) 2007 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Virology |
| 类目[WOS] | Virology |
| 研究领域[WOS] | Virology |
| 关键词[WOS] | VESICULAR STOMATITIS-VIRUS ; HEMATOPOIETIC NECROSIS VIRUS ; COMPLETE NUCLEOTIDE-SEQUENCE ; HEMORRHAGIC SEPTICEMIA VIRUS ; EPHEMERAL FEVER RHABDOVIRUS ; RNA-POLYMERASE ; MOLECULAR CHARACTERIZATION ; GENE JUNCTIONS ; VIRAL-RNA ; N PROTEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000254109100010 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8216]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Tao, Jian-Jun,Zhou, Guang-Zhou,Gui, Han-Farig,et al. Genomic sequence of mandarin fish rhabdovirus with an unusual small non-transcriptional ORF[J]. VIRUS RESEARCH,2008,132(1-2):86-96. |
| APA | Tao, Jian-Jun,Zhou, Guang-Zhou,Gui, Han-Farig,&Zhang, Qi-Ya.(2008).Genomic sequence of mandarin fish rhabdovirus with an unusual small non-transcriptional ORF.VIRUS RESEARCH,132(1-2),86-96. |
| MLA | Tao, Jian-Jun,et al."Genomic sequence of mandarin fish rhabdovirus with an unusual small non-transcriptional ORF".VIRUS RESEARCH 132.1-2(2008):86-96. |
入库方式： OAI收割
来源：水生生物研究所
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