The role of glutathione metabolism in tolerance of tobacco BY-2 suspension cells to microcystin-RR
文献类型：期刊论文
| 作者 | Huang, Wen Min1,2; Xing, Wei3; Li, Dun Hai1; Liu, Yong Ding1 |
| 刊名 | BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY
 |
| 出版日期 | 2008-03-01 |
| 卷号 | 80期号:3页码:215-219 |
| 关键词 | Microcystin-RR tobacco BY-2 suspension cells glutathione metabolism oxidative stress |
| ISSN号 | 0007-4861 |
| 通讯作者 | Liu, YD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | This study was undertaken to investigate the role of the glutathione-involved detoxifying mechanism in defending the tobacco BY-2 suspension cells against microcystin-RR (MC-RR). Analysis showed that exposure of the cells to different concentrations of MC-RR (0.1, 1 and 10 mu g/mL) for 0-6 days resulted in a time and concentration-dependent decrease in cell viability and increase in reactive oxygen species (ROS) content. Reduced glutathione (GSH) and total glutathione (tGSH) content as well as glutathione reductase (GR), glutathione peroxidase (GPX) and glutathione-S-transferase (GST) activities significantly increased after 3-4 days exposure in the highest two concentration treated groups, while decreased until reaching the control values except for GPX at day 6. Oxidized glutathione (GSSG) content markedly increased compared with control in high concentration MC-RR treated group after 6 days exposure. The GSH/GSSG ratio was much higher than control in 10 mu g/mL MC-RR treated group at day 4, but after 6 days exposure, the ratios in all treated groups were lower than that of the control group. |
| 英文摘要 | This study was undertaken to investigate the role of the glutathione-involved detoxifying mechanism in defending the tobacco BY-2 suspension cells against microcystin-RR (MC-RR). Analysis showed that exposure of the cells to different concentrations of MC-RR (0.1, 1 and 10 mu g/mL) for 0-6 days resulted in a time and concentration-dependent decrease in cell viability and increase in reactive oxygen species (ROS) content. Reduced glutathione (GSH) and total glutathione (tGSH) content as well as glutathione reductase (GR), glutathione peroxidase (GPX) and glutathione-S-transferase (GST) activities significantly increased after 3-4 days exposure in the highest two concentration treated groups, while decreased until reaching the control values except for GPX at day 6. Oxidized glutathione (GSSG) content markedly increased compared with control in high concentration MC-RR treated group after 6 days exposure. The GSH/GSSG ratio was much higher than control in 10 mu g/mL MC-RR treated group at day 4, but after 6 days exposure, the ratios in all treated groups were lower than that of the control group. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Environmental Sciences; Toxicology |
| 类目[WOS] | Environmental Sciences ; Toxicology |
| 研究领域[WOS] | Environmental Sciences & Ecology ; Toxicology |
| 关键词[WOS] | OXIDATIVE STRESS ; LR ; DEFENSE ; SYSTEM |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000253993500008 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8226]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Chinese Acad Sci, Wuhan Bot Garden, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Wen Min,Xing, Wei,Li, Dun Hai,et al. The role of glutathione metabolism in tolerance of tobacco BY-2 suspension cells to microcystin-RR[J]. BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY,2008,80(3):215-219. |
| APA | Huang, Wen Min,Xing, Wei,Li, Dun Hai,&Liu, Yong Ding.(2008).The role of glutathione metabolism in tolerance of tobacco BY-2 suspension cells to microcystin-RR.BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY,80(3),215-219. |
| MLA | Huang, Wen Min,et al."The role of glutathione metabolism in tolerance of tobacco BY-2 suspension cells to microcystin-RR".BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY 80.3(2008):215-219. |
入库方式： OAI收割
来源：水生生物研究所
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