Bifunctional enzyme FBPase/SBPase is essential for photoautotrophic growth in cyanobacterium Synechocystis sp PCC 6803
文献类型：期刊论文
| 作者 | Yan, Chunlan; Xu, Xudong |
| 刊名 | PROGRESS IN NATURAL SCIENCE
 |
| 出版日期 | 2008-02-10 |
| 卷号 | 18期号:2页码:149-153 |
| 关键词 | FBPase photoautotrophic growth light sensitivity Synechocystis sp. |
| ISSN号 | 1002-0071 |
| 通讯作者 | Xu, XD, Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | From a random insertion mutant library of Synechocystis sp. PCC 6803, a mutant defective in photoautotrophic growth was obtained. The interrupted gene was identified to be slr2094 (rbpl), which encodes the fructose-1,6-biphosphatase (FBPase)/sedoheptulose-1,7-biphosphatase (SBPase) bifunctional enzyme (F-I). Two other independently constructed slr2094 mutants showed an identical phenotype. The FBPase activity was found to be virtually lacking in an slr2094 mutant, which was sensitive to light under mixotrophic growth conditions. These results indicate that slr2094 is the only active FBPase-encoding gene in this cyanobacterium. Inactivation of photosystem II by interrupting psbB in slr2094 mutant alleviated the sensitiveness to light. This report provides the direct genetic evidence for the essential role of F-I in the photosynthesis of Synechocystis sp. PCC 6803. (c) 2007 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved. |
| 英文摘要 | From a random insertion mutant library of Synechocystis sp. PCC 6803, a mutant defective in photoautotrophic growth was obtained. The interrupted gene was identified to be slr2094 (rbpl), which encodes the fructose-1,6-biphosphatase (FBPase)/sedoheptulose-1,7-biphosphatase (SBPase) bifunctional enzyme (F-I). Two other independently constructed slr2094 mutants showed an identical phenotype. The FBPase activity was found to be virtually lacking in an slr2094 mutant, which was sensitive to light under mixotrophic growth conditions. These results indicate that slr2094 is the only active FBPase-encoding gene in this cyanobacterium. Inactivation of photosystem II by interrupting psbB in slr2094 mutant alleviated the sensitiveness to light. This report provides the direct genetic evidence for the essential role of F-I in the photosynthesis of Synechocystis sp. PCC 6803. (c) 2007 National Natural Science Foundation of China and Chinese Academy of Sciences. Published by Elsevier Limited and Science in China Press. All rights reserved. |
| WOS标题词 | Science & Technology ; Technology |
| 学科主题 | Multidisciplinary Sciences |
| 类目[WOS] | Materials Science, Multidisciplinary ; Multidisciplinary Sciences |
| 研究领域[WOS] | Materials Science ; Science & Technology - Other Topics |
| 关键词[WOS] | FRUCTOSE 1,6-BISPHOSPHATASE ; SYNECHOCOCCUS-LEOPOLIENSIS ; MOLECULAR CHARACTERIZATION ; HYDROGEN-PEROXIDE ; PHOTOSYSTEM-II ; PCC 6803 ; CHLOROPLAST ; FRUCTOSE-1,6-BISPHOSPHATASE ; PURIFICATION ; PROTEIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000253767200003 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8238]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yan, Chunlan,Xu, Xudong. Bifunctional enzyme FBPase/SBPase is essential for photoautotrophic growth in cyanobacterium Synechocystis sp PCC 6803[J]. PROGRESS IN NATURAL SCIENCE,2008,18(2):149-153. |
| APA | Yan, Chunlan,&Xu, Xudong.(2008).Bifunctional enzyme FBPase/SBPase is essential for photoautotrophic growth in cyanobacterium Synechocystis sp PCC 6803.PROGRESS IN NATURAL SCIENCE,18(2),149-153. |
| MLA | Yan, Chunlan,et al."Bifunctional enzyme FBPase/SBPase is essential for photoautotrophic growth in cyanobacterium Synechocystis sp PCC 6803".PROGRESS IN NATURAL SCIENCE 18.2(2008):149-153. |
入库方式： OAI收割
来源：水生生物研究所
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