In vitro and in vivo evaluations on osteogenesis and biodegradability of a ss-tricalcium phosphate coated magnesium alloy
文献类型：期刊论文
| 作者 | H. W. Chai ; L. Guo ; X. T. Wang ; X. Y. Gao ; K. Liu ; Y. P. Fu ; J. L. Guan ; L. L. Tan ; K. Yang |
| 刊名 | Journal of Biomedical Materials Research Part A
 |
| 出版日期 | 2012 |
| 卷号 | 100A期号:2页码:293-304 |
| 关键词 | magnesium alloy ss-tricalcium phosphate osteogenesis corrosion resistance bone morphogenetic protein-2 mechanical integrity cell compatibility calcium silicate ca alloys beta-tcp zn alloy hydroxyapatite degradation composite |
| ISSN号 | 1549-3296 |
| 中文摘要 | Magnesium (Mg) alloys have been demonstrated to be potential orthopedic implants due to their biodegradability in vivo. To enhance its corrosion resistance and improve its osteogenesis, beta-tricalcium phosphate (beta-TCP) was coated on a Mg alloy (Mg-3AI-1Zn) by phosphating process. In vitro, the human osteosarcoma cell line (SaOS-2) showed significantly good adherence and proliferation on the surface of the beta-TCP coated Mg alloy after 24-h incubation. The growth factor bone morphogenetic protein 2 (BMP-2) was highly expressed in SaOS-2 cultured with the beta-TCP coated Mg alloy by Western blot analysis (p < 0.05). In vivo, the newborn bone at the implant/bone interface was formed at week 1 and matured at week 4 postimplantation. Villous tissue was found at the implant/bone interface at week 12 postimplantation. The contents of phosphorus and calcium on the surface of the beta-TCP coated Mg alloy were decreased at week 4 and week 12 postimplantation, respectively. Immunohistochemical analysis of the experiment results demonstrated that the beta-TCP coated Mg alloy implants provided a high BMP-2 expression during the first 4 weeks postimplantation. Compared with the naked Mg alloy which was degraded for 33% in vivo, only 17% of the beta-TCP coated Mg alloy was degraded at week 12 postimplantation (p < 0.05). The in vitro cell tests showed that the beta-TCP coating provided the Mg alloy with a significantly better surface cytocompatibility, and in vivo results also confirmed that the beta-TCP coating exhibited greatly improved osteoconductivity and osteogenesis in the early 12 weeks postoperation period. Moreover, in vivo experiment demonstrated that the beta-TCP coating layer could slow down the degradation of the naked Mg alloy at the early stage of implantation. (C) 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012. |
| 原文出处 | |
| 公开日期 | 2013-02-05 |
| 源URL | [http://ir.imr.ac.cn/handle/321006/59891]  |
| 专题 | 金属研究所_中国科学院金属研究所 |
| 推荐引用方式 GB/T 7714 | H. W. Chai,L. Guo,X. T. Wang,et al. In vitro and in vivo evaluations on osteogenesis and biodegradability of a ss-tricalcium phosphate coated magnesium alloy[J]. Journal of Biomedical Materials Research Part A,2012,100A(2):293-304. |
| APA | H. W. Chai.,L. Guo.,X. T. Wang.,X. Y. Gao.,K. Liu.,...&K. Yang.(2012).In vitro and in vivo evaluations on osteogenesis and biodegradability of a ss-tricalcium phosphate coated magnesium alloy.Journal of Biomedical Materials Research Part A,100A(2),293-304. |
| MLA | H. W. Chai,et al."In vitro and in vivo evaluations on osteogenesis and biodegradability of a ss-tricalcium phosphate coated magnesium alloy".Journal of Biomedical Materials Research Part A 100A.2(2012):293-304. |
入库方式： OAI收割
来源：金属研究所
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