A novel chemiluminescent immunoassay for microcystin (MC) detection based on gold nanoparticles label and its application to MC analysis in aquatic environmental samples
文献类型：期刊论文
| 作者 | Hu, Chenlin1,2; Gan, Nanqin1; He, Zhike3; Song, Lirong1 |
| 刊名 | INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY
 |
| 出版日期 | 2008 |
| 卷号 | 88期号:4页码:267-277 |
| 关键词 | microcystins gold nanoparticles chemiluminescence ELISA |
| ISSN号 | 0306-7319 |
| 通讯作者 | Song, LR, CAS, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Donghu Nanlu 7, Wuhan 430072, Peoples R China |
| 中文摘要 | A novel chemiluminescent immunoassay method based on gold nanoparticles was developed for the detection of microcystins (MCs). The immunoassay included three main steps: indirect competitive immunoreaction, oxidative dissolution of gold nanoparticles, and indirect determination for MCs with Au3+-catalysed luminol chemiluminesent system. The method has a wide working range (0.05-10 mu g L-1, r(2) = 0.9914), the limit of detection was determined to be 0.024 mu g L-1, which is much lower than the World Health Organization's proposed guidelines (1 mu g L-1) for drinking-water. The proposed method was applied to MC analysis in natural water and fish tissue samples, and most results in the proposed method were in agreement with the conventional indirect competitive enzyme-linked immunosorbent assay method, which indicated that the new chemiluminescent immunoassay was sensitive, reliable, and suitable for MC analysis in natural water and fish tissue samples. |
| 英文摘要 | A novel chemiluminescent immunoassay method based on gold nanoparticles was developed for the detection of microcystins (MCs). The immunoassay included three main steps: indirect competitive immunoreaction, oxidative dissolution of gold nanoparticles, and indirect determination for MCs with Au3+-catalysed luminol chemiluminesent system. The method has a wide working range (0.05-10 mu g L-1, r(2) = 0.9914), the limit of detection was determined to be 0.024 mu g L-1, which is much lower than the World Health Organization's proposed guidelines (1 mu g L-1) for drinking-water. The proposed method was applied to MC analysis in natural water and fish tissue samples, and most results in the proposed method were in agreement with the conventional indirect competitive enzyme-linked immunosorbent assay method, which indicated that the new chemiluminescent immunoassay was sensitive, reliable, and suitable for MC analysis in natural water and fish tissue samples. |
| WOS标题词 | Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine |
| 学科主题 | Chemistry ; Analytical; Environmental Sciences |
| 类目[WOS] | Chemistry, Analytical ; Environmental Sciences |
| 研究领域[WOS] | Chemistry ; Environmental Sciences & Ecology |
| 关键词[WOS] | MASS-SPECTROMETRY ; WATER ; ANTIBODIES ; ALGAE ; ELISA ; QUANTIFICATION ; CYANOBACTERIA ; HEPATOTOXINS ; DEPOSITION ; NODULARIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000253658600004 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8244]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.CAS, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Chenlin,Gan, Nanqin,He, Zhike,et al. A novel chemiluminescent immunoassay for microcystin (MC) detection based on gold nanoparticles label and its application to MC analysis in aquatic environmental samples[J]. INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY,2008,88(4):267-277. |
| APA | Hu, Chenlin,Gan, Nanqin,He, Zhike,&Song, Lirong.(2008).A novel chemiluminescent immunoassay for microcystin (MC) detection based on gold nanoparticles label and its application to MC analysis in aquatic environmental samples.INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY,88(4),267-277. |
| MLA | Hu, Chenlin,et al."A novel chemiluminescent immunoassay for microcystin (MC) detection based on gold nanoparticles label and its application to MC analysis in aquatic environmental samples".INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY 88.4(2008):267-277. |
入库方式： OAI收割
来源：水生生物研究所
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