alpha-tocopherol is essential for acquired chill-light tolerance in the cyanobacterium Synechocystis sp strain PCC 6803
文献类型：期刊论文
| 作者 | Yang, Yang; Yin, Chuntao; Li, Weizhi; Xu, Xudong |
| 刊名 | JOURNAL OF BACTERIOLOGY
 |
| 出版日期 | 2008-03-01 |
| 卷号 | 190期号:5页码:1554-1560 |
| 关键词 | LIPID-PEROXIDATION VITAMIN-E HOMOGENTISATE PHYTYLTRANSFERASE MICROCYSTIS COLD ARABIDOPSIS PLANTS BIOSYNTHESIS EXPRESSION VECTORS |
| ISSN号 | 0021-9193 |
| 通讯作者 | Xu, XD, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China |
| 中文摘要 | Unlike Escherichia coli, the cyanobacterium Synechocystis sp. strain PCC 6803 is insensitive to chill (5 degrees C) in the dark but rapidly losses viability when exposed to chill in the light (100 mu mol photons m(-2) s(-1)). Preconditioning at a low temperature (15 degrees C) greatly enhances the chill-light tolerance of Synechocystis sp. strain PCC 6803. This phenomenon is called acquired chill-light tolerance (ACLT). Preconditioned wild-type cells maintained a substantially higher level of alpha-tocopherol after exposure to chill-light stress. Mutants unable to synthesize alpha-tocopherol, such as slr1736, slr1737, slr0089, and slr0090 mutants, almost completely lost ACLT. When exposed to chill without light, these mutants showed no or a slight difference from the wild type. When complemented, the slr0089 mutant regained its ACLT. Copper-regulated expression of slr0090 from P-petE controlled the level of et-tocopherol and ACLT. We conclude that alpha-tocopherol is essential for ACLT of Synechocystis sp. strain PCC 6803. The role of a-tocopherol in ACLT may be based largely on a nonantioxidant activity that is not possessed by other tocopherols or pathway intermediates. |
| 英文摘要 | Unlike Escherichia coli, the cyanobacterium Synechocystis sp. strain PCC 6803 is insensitive to chill (5 degrees C) in the dark but rapidly losses viability when exposed to chill in the light (100 mu mol photons m(-2) s(-1)). Preconditioning at a low temperature (15 degrees C) greatly enhances the chill-light tolerance of Synechocystis sp. strain PCC 6803. This phenomenon is called acquired chill-light tolerance (ACLT). Preconditioned wild-type cells maintained a substantially higher level of alpha-tocopherol after exposure to chill-light stress. Mutants unable to synthesize alpha-tocopherol, such as slr1736, slr1737, slr0089, and slr0090 mutants, almost completely lost ACLT. When exposed to chill without light, these mutants showed no or a slight difference from the wild type. When complemented, the slr0089 mutant regained its ACLT. Copper-regulated expression of slr0090 from P-petE controlled the level of et-tocopherol and ACLT. We conclude that alpha-tocopherol is essential for ACLT of Synechocystis sp. strain PCC 6803. The role of a-tocopherol in ACLT may be based largely on a nonantioxidant activity that is not possessed by other tocopherols or pathway intermediates. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Microbiology |
| 类目[WOS] | Microbiology |
| 研究领域[WOS] | Microbiology |
| 关键词[WOS] | LIPID-PEROXIDATION ; VITAMIN-E ; HOMOGENTISATE PHYTYLTRANSFERASE ; MICROCYSTIS ; COLD ; ARABIDOPSIS ; PLANTS ; BIOSYNTHESIS ; EXPRESSION ; VECTORS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000253554300008 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8252]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Yang,Yin, Chuntao,Li, Weizhi,et al. alpha-tocopherol is essential for acquired chill-light tolerance in the cyanobacterium Synechocystis sp strain PCC 6803[J]. JOURNAL OF BACTERIOLOGY,2008,190(5):1554-1560. |
| APA | Yang, Yang,Yin, Chuntao,Li, Weizhi,&Xu, Xudong.(2008).alpha-tocopherol is essential for acquired chill-light tolerance in the cyanobacterium Synechocystis sp strain PCC 6803.JOURNAL OF BACTERIOLOGY,190(5),1554-1560. |
| MLA | Yang, Yang,et al."alpha-tocopherol is essential for acquired chill-light tolerance in the cyanobacterium Synechocystis sp strain PCC 6803".JOURNAL OF BACTERIOLOGY 190.5(2008):1554-1560. |
入库方式： OAI收割
来源：水生生物研究所
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