Constitutive expression of thymidylate synthase from LCDV-C induces a transformed phenotype in fish cells
文献类型：期刊论文
| 作者 | Zhao, Zhe; Shi, Yan; Ke, Fei; Wei, Sun; Gui, Hanfang; Zhang, Qiya |
| 刊名 | VIROLOGY
 |
| 出版日期 | 2008-03-01 |
| 卷号 | 372期号:1页码:118-126 |
| 关键词 | thymidylate synthase lymphocystis disease virus from China (LCDV-C) cell transformation foci formation anchorage-independent growth |
| ISSN号 | 0042-6822 |
| 通讯作者 | Zhang, QY, Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Thymidylate synthase (TS), an essential enzyme in DNA synthesis and repair, plays a key role in the events of cell cycle regulation and tumor formation. Here, an investigation was presented about subcellular location and biological function of viral TS from lymphocystis disease virus from China (LCDV-C) in fish cells. Fluorescence microscopy revealed that LCDV-C TS was predominantly localized in the cytoplasm in fish cells. Cell cycle analysis demonstrated that LCDV-C TS promoted cell cycle progression into S and G2/M phase in the constitutive expressed cells. As a result, the cells have a faster growth rate compared with the control cells as revealed by cell growth curves. For foci assay, the TS-expressed cells gave rise to foci 4-5 weeks after incubation. Microscopic examination of the TS-induced foci revealed multilayered growth and crisscross morphology characteristic of transformed cells. Moreover, LCDV-C TS predisposed the transfected cells to acquire an anchorage-independent phenotype and could grow in 0.3% soft agar. So the data reveal LCDV-C TS is sufficient to induce a transformed phenotype in fish cells in vitro and exhibits its potential ability in cell transformation. To our knowledge, it is the first report on viral TS sequences associated with transforming activity. (C) 2007 Elsevier Inc. All rights reserved. |
| 英文摘要 | Thymidylate synthase (TS), an essential enzyme in DNA synthesis and repair, plays a key role in the events of cell cycle regulation and tumor formation. Here, an investigation was presented about subcellular location and biological function of viral TS from lymphocystis disease virus from China (LCDV-C) in fish cells. Fluorescence microscopy revealed that LCDV-C TS was predominantly localized in the cytoplasm in fish cells. Cell cycle analysis demonstrated that LCDV-C TS promoted cell cycle progression into S and G2/M phase in the constitutive expressed cells. As a result, the cells have a faster growth rate compared with the control cells as revealed by cell growth curves. For foci assay, the TS-expressed cells gave rise to foci 4-5 weeks after incubation. Microscopic examination of the TS-induced foci revealed multilayered growth and crisscross morphology characteristic of transformed cells. Moreover, LCDV-C TS predisposed the transfected cells to acquire an anchorage-independent phenotype and could grow in 0.3% soft agar. So the data reveal LCDV-C TS is sufficient to induce a transformed phenotype in fish cells in vitro and exhibits its potential ability in cell transformation. To our knowledge, it is the first report on viral TS sequences associated with transforming activity. (C) 2007 Elsevier Inc. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Virology |
| 类目[WOS] | Virology |
| 研究领域[WOS] | Virology |
| 关键词[WOS] | LYMPHOCYSTIS DISEASE VIRUS ; COMPLETE DNA-SEQUENCE ; GENE-EXPRESSION ; PARALICHTHYS-OLIVACEUS ; FAMILY IRIDOVIRIDAE ; COMPLETE GENOME ; PROPAGATION ; PROTEIN ; ENZYME ; CHINA |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000253325300012 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8260]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | Chinese Acad Sci, Grad Sch, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Zhe,Shi, Yan,Ke, Fei,et al. Constitutive expression of thymidylate synthase from LCDV-C induces a transformed phenotype in fish cells[J]. VIROLOGY,2008,372(1):118-126. |
| APA | Zhao, Zhe,Shi, Yan,Ke, Fei,Wei, Sun,Gui, Hanfang,&Zhang, Qiya.(2008).Constitutive expression of thymidylate synthase from LCDV-C induces a transformed phenotype in fish cells.VIROLOGY,372(1),118-126. |
| MLA | Zhao, Zhe,et al."Constitutive expression of thymidylate synthase from LCDV-C induces a transformed phenotype in fish cells".VIROLOGY 372.1(2008):118-126. |
入库方式： OAI收割
来源：水生生物研究所
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