Cloning and characterization of interferon stimulated genes Viperin and ISG15, and their promoters from snakehead Channa argus
文献类型：期刊论文
| 作者 | Jia Weizhang1,2; Zhou Xiuxia1,2; Huang Rong1,2; Guo Qionglin1 |
| 刊名 | PROGRESS IN NATURAL SCIENCE
 |
| 出版日期 | 2007-12-01 |
| 卷号 | 17期号:12页码:1425-1435 |
| ISSN号 | 1002-0071 |
| 关键词 | interferon interferon stimulated gene (ISG) Viperin ISG15 snakehead (Channa argus) |
| 通讯作者 | Guo, QL, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China |
| 中文摘要 | By suppression subtractive hybridization, rapid amplification of cDNA ends and gene walking methods, interferon stimulated genes (ISGs), Viperin and ISG15, and their promoters have been cloned and characterized from snakehead Channa argus. The Viperin cDNA was found to be 1474 nt and contain an open reading frame (ORF) of 1059 nt that translates into a putative peptide of 352 amino acid (aa). The putative peptide of Viperin shows high identity to that in teleosts and mammals except for the N-terminal 70 aa. The ISG15 cDNA was found to be 758 nt and contain an ORF of 468 nt that translates into a putative peptide of 155 aa. The putative peptide of ISG15 is composed of two tandem repeats of ubiquitin-like (UBL) domains, and a canonical conjugation motif (LRGG) at C-terminal. Viperin and ISG15 promoter regions were characterized by the presence of interferon stimulating response elements (ISRE) and gamma-IFN activation sites (GAS). ISRE is a feature of IFN-induced gene promoter and partially overlaps interferon regulatory factor (IRF) 1 and IRF2 recognition sites. GAS is responsible for the gamma-IFN mediated transcription. One conserved site for NF-kappa B was found in the promoter region of Viperin. This is the first report of conservative binding motif for NF-kappa B in accordance with the consensus sequence (GGGRN-NYYCC) among teleost ISG promoters. Moreover, there were also TATA, CAAT and Sp1 transcription factor sites in Viperin and ISG15 promoters. In 5' untranslated region (UTR), snakehead ISG15 gene contains a single intron, which differs from Viperin gene. The transcripts of Vipeirn and ISG15 mRNA were mainly expressed in head kidney, posterior kidney, spleen and gill. The expression levels in liver were found to increase obviously in response to induction by IFN-inducer poly I : C. |
| 英文摘要 | By suppression subtractive hybridization, rapid amplification of cDNA ends and gene walking methods, interferon stimulated genes (ISGs), Viperin and ISG15, and their promoters have been cloned and characterized from snakehead Channa argus. The Viperin cDNA was found to be 1474 nt and contain an open reading frame (ORF) of 1059 nt that translates into a putative peptide of 352 amino acid (aa). The putative peptide of Viperin shows high identity to that in teleosts and mammals except for the N-terminal 70 aa. The ISG15 cDNA was found to be 758 nt and contain an ORF of 468 nt that translates into a putative peptide of 155 aa. The putative peptide of ISG15 is composed of two tandem repeats of ubiquitin-like (UBL) domains, and a canonical conjugation motif (LRGG) at C-terminal. Viperin and ISG15 promoter regions were characterized by the presence of interferon stimulating response elements (ISRE) and gamma-IFN activation sites (GAS). ISRE is a feature of IFN-induced gene promoter and partially overlaps interferon regulatory factor (IRF) 1 and IRF2 recognition sites. GAS is responsible for the gamma-IFN mediated transcription. One conserved site for NF-kappa B was found in the promoter region of Viperin. This is the first report of conservative binding motif for NF-kappa B in accordance with the consensus sequence (GGGRN-NYYCC) among teleost ISG promoters. Moreover, there were also TATA, CAAT and Sp1 transcription factor sites in Viperin and ISG15 promoters. In 5' untranslated region (UTR), snakehead ISG15 gene contains a single intron, which differs from Viperin gene. The transcripts of Vipeirn and ISG15 mRNA were mainly expressed in head kidney, posterior kidney, spleen and gill. The expression levels in liver were found to increase obviously in response to induction by IFN-inducer poly I : C. |
| 学科主题 | Multidisciplinary Sciences |
| WOS标题词 | Science & Technology ; Technology |
| 类目[WOS] | Materials Science, Multidisciplinary ; Multidisciplinary Sciences |
| 研究领域[WOS] | Materials Science ; Science & Technology - Other Topics |
| 关键词[WOS] | TROUT ONCORHYNCHUS-MYKISS ; RAINBOW-TROUT ; MOLECULAR-CLONING ; FUNCTIONAL-CHARACTERIZATION ; RESPONSIVE GENES ; MX-GENE ; FISH ; VIRUS ; EXPRESSION ; INFECTION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000253164300006 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8262]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China 2.Grad Univ, Chinese Acad Sci, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jia Weizhang,Zhou Xiuxia,Huang Rong,et al. Cloning and characterization of interferon stimulated genes Viperin and ISG15, and their promoters from snakehead Channa argus[J]. PROGRESS IN NATURAL SCIENCE,2007,17(12):1425-1435. |
| APA | Jia Weizhang,Zhou Xiuxia,Huang Rong,&Guo Qionglin.(2007).Cloning and characterization of interferon stimulated genes Viperin and ISG15, and their promoters from snakehead Channa argus.PROGRESS IN NATURAL SCIENCE,17(12),1425-1435. |
| MLA | Jia Weizhang,et al."Cloning and characterization of interferon stimulated genes Viperin and ISG15, and their promoters from snakehead Channa argus".PROGRESS IN NATURAL SCIENCE 17.12(2007):1425-1435. |
入库方式： OAI收割
来源：水生生物研究所
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