Construction of cytoplasmic molecular markers distinguishing Danio rerio from Gobiocypris rarus at high identity domains based on MP-PCR strategy and Sybr Green I detection
文献类型：期刊论文
| 作者 | Pei, De-Sheng1,2; Sun, Yong-Hua1; Zhu, Zuo-Yan1 |
| 刊名 | MOLECULAR BIOLOGY REPORTS
 |
| 出版日期 | 2008-03-01 |
| 卷号 | 35期号:1页码:45-50 |
| 关键词 | COXI Danio rerio Gobiocypris rarus MP-PCR sybr Green I detection |
| ISSN号 | 0301-4851 |
| 通讯作者 | Zhu, ZY, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | To distinguish the cytoplasm of Danio rerio from that of Gobiocypris rarus, we cloned G. rarus COXI and constructed cytoplasmic molecular markers at the high identity domains of COXI by mutated primer PCR (MP-PCR for short). Then Sybr Green I was used to detect the single amplicon. As a result, we succeeded in getting the cytoplasmic molecular markers, G.M COXI and Z.M COXI, by MP-PCR strategy. They were used to detect the sperm-derived mtDNA in the sexual hybrid embryos (D. rerio female x G. rarus male) before the sphere stage. In the present study, all results demonstrate that MP-PCR approach and Sybr Green I detection are feasible to construct the molecular markers to identify genes that shared high identity. |
| 英文摘要 | To distinguish the cytoplasm of Danio rerio from that of Gobiocypris rarus, we cloned G. rarus COXI and constructed cytoplasmic molecular markers at the high identity domains of COXI by mutated primer PCR (MP-PCR for short). Then Sybr Green I was used to detect the single amplicon. As a result, we succeeded in getting the cytoplasmic molecular markers, G.M COXI and Z.M COXI, by MP-PCR strategy. They were used to detect the sperm-derived mtDNA in the sexual hybrid embryos (D. rerio female x G. rarus male) before the sphere stage. In the present study, all results demonstrate that MP-PCR approach and Sybr Green I detection are feasible to construct the molecular markers to identify genes that shared high identity. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 学科主题 | Biochemistry & Molecular Biology |
| 类目[WOS] | Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | POLYMERASE CHAIN-REACTION ; CELL NUCLEAR TRANSFER ; MITOCHONDRIAL-DNA ; SOMATIC-CELL ; AMPLIFICATION ; ZEBRAFISH |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000252541400007 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8286]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Grp Environm Genom, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pei, De-Sheng,Sun, Yong-Hua,Zhu, Zuo-Yan. Construction of cytoplasmic molecular markers distinguishing Danio rerio from Gobiocypris rarus at high identity domains based on MP-PCR strategy and Sybr Green I detection[J]. MOLECULAR BIOLOGY REPORTS,2008,35(1):45-50. |
| APA | Pei, De-Sheng,Sun, Yong-Hua,&Zhu, Zuo-Yan.(2008).Construction of cytoplasmic molecular markers distinguishing Danio rerio from Gobiocypris rarus at high identity domains based on MP-PCR strategy and Sybr Green I detection.MOLECULAR BIOLOGY REPORTS,35(1),45-50. |
| MLA | Pei, De-Sheng,et al."Construction of cytoplasmic molecular markers distinguishing Danio rerio from Gobiocypris rarus at high identity domains based on MP-PCR strategy and Sybr Green I detection".MOLECULAR BIOLOGY REPORTS 35.1(2008):45-50. |
入库方式： OAI收割
来源：水生生物研究所
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