中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Increasing the genetic uniformity of bighead carp [Aristichthys nobilis (Richardson)] by means of spontaneous diploidization of gynogenetically activated eggs

文献类型:期刊论文

作者YuzhenYe; Wang, Zhongwei; Wu, Qingjiang
刊名AQUACULTURE RESEARCH
出版日期2008-01-23
卷号39期号:2页码:205-211
关键词gynogenesis spontaneous diploidization chromosome genetic identity
ISSN号1355-557X
通讯作者Wu, QJ, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
中文摘要Three groups of gynogenetic diploid bighead carp were successfully obtained by means of artificial gynogenesis. The activation rates of gynogenesis varied from 75.9% to 98.8%, and the frequency of spontaneous diploidization was around 0.4%. Over 2000 normally gynogenetic diploid fry were obtained in three gynogenetic groups. The haploid karyotype consisted of nine metacentric, 12 submetacentric, three subtelocentric chromosomes and 45 arms. The chromosome number was 48 from gynogenetic diploid. The results showed that the genetic material of offspring was maternal. The aneuploid hybrid embryos of bighead carp and Xingguo red common carp with chromosome numbers ranging from 28 to 73 did not survive post hatch, likely the result of incompatibility between the nucleus and the cytoplasm of two parents. Sixty RAPD primers from three groups were used for total DNA amplification of gynogenetic offspring, maternal and 'paternal' fish. A total of 451 bands were amplified from three kinds of samples above. From maternal bighead carp, 256 bands were amplified; however, there were 251 shared bands between maternal and gynogenetic bighead carp. From artificial gynogenetic offspring, two 'paternal' DNA segments without an expression function were found. An UPGMA tree showed that gynogenetic offspring were closely clustered and the genetic identity among them was very high (0.956).
英文摘要Three groups of gynogenetic diploid bighead carp were successfully obtained by means of artificial gynogenesis. The activation rates of gynogenesis varied from 75.9% to 98.8%, and the frequency of spontaneous diploidization was around 0.4%. Over 2000 normally gynogenetic diploid fry were obtained in three gynogenetic groups. The haploid karyotype consisted of nine metacentric, 12 submetacentric, three subtelocentric chromosomes and 45 arms. The chromosome number was 48 from gynogenetic diploid. The results showed that the genetic material of offspring was maternal. The aneuploid hybrid embryos of bighead carp and Xingguo red common carp with chromosome numbers ranging from 28 to 73 did not survive post hatch, likely the result of incompatibility between the nucleus and the cytoplasm of two parents. Sixty RAPD primers from three groups were used for total DNA amplification of gynogenetic offspring, maternal and 'paternal' fish. A total of 451 bands were amplified from three kinds of samples above. From maternal bighead carp, 256 bands were amplified; however, there were 251 shared bands between maternal and gynogenetic bighead carp. From artificial gynogenetic offspring, two 'paternal' DNA segments without an expression function were found. An UPGMA tree showed that gynogenetic offspring were closely clustered and the genetic identity among them was very high (0.956).
WOS标题词Science & Technology ; Life Sciences & Biomedicine
学科主题Fisheries
类目[WOS]Fisheries
研究领域[WOS]Fisheries
关键词[WOS]RAINBOW-TROUT ; GYNOGENESIS ; MANIPULATION
收录类别SCI
语种英语
WOS记录号WOS:000252586800011
公开日期2010-10-13
源URL[http://ir.ihb.ac.cn/handle/152342/8294]  
专题水生生物研究所_中科院水生所知识产出(2009年前)_期刊论文
作者单位Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Peoples R China
推荐引用方式
GB/T 7714
YuzhenYe,Wang, Zhongwei,Wu, Qingjiang. Increasing the genetic uniformity of bighead carp [Aristichthys nobilis (Richardson)] by means of spontaneous diploidization of gynogenetically activated eggs[J]. AQUACULTURE RESEARCH,2008,39(2):205-211.
APA YuzhenYe,Wang, Zhongwei,&Wu, Qingjiang.(2008).Increasing the genetic uniformity of bighead carp [Aristichthys nobilis (Richardson)] by means of spontaneous diploidization of gynogenetically activated eggs.AQUACULTURE RESEARCH,39(2),205-211.
MLA YuzhenYe,et al."Increasing the genetic uniformity of bighead carp [Aristichthys nobilis (Richardson)] by means of spontaneous diploidization of gynogenetically activated eggs".AQUACULTURE RESEARCH 39.2(2008):205-211.

入库方式: OAI收割

来源:水生生物研究所

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