Molecular cloning and characterization of carp (Cyprinus carpio L.) CD8 beta and CD4-like genes
文献类型：期刊论文
| 作者 | Sun, Xiao-Feng; Shang, Na; Hu, Wei; Wang, Ya-Ping; Guo, Qiong-Lin |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2007-12-01 |
| 卷号 | 23期号:6页码:1242-1255 |
| ISSN号 | 1050-4648 |
| 关键词 | CD8 beta CD4 T cells cell surface molecule common carp |
| 通讯作者 | Guo, QL, Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China |
| 中文摘要 | Partial cDNA sequences of both CD8 beta and CD4-like (CD4L) genes of common carp (Cyprinus carpio L.) were isolated from thymus cDNA library by the method of suppression subtractive hybridization (SSH). Subsequently the full length cDNAs of carp CD8 and CD4L were obtained by means of 3' RACE and 5' RACE, respectively. The full length cDNA of carp CD8 is 1164 bp and encodes 207 amino acids including a signal peptide region of 24 amino acids, a transmembrane region of 23 amino acids from aa 167 to aa189 and an immunoglobulin V-set from aa 19 to aa 141. Similar to other species CD8 beta s,carp CD8 beta also lacks p56(lck) domain in the cytoplasmic region. The full length cDNA of carp CD4L is 2001 bp and encodes 458 amino acids including four immunoglobulin (Ig)-like domains in the extracellular region, a transmembrane region of 23 amino acids at the C-terminal region from aa 402 to aa 424 and a cytoplasmic tail. Similar to mammalian, avian CD4s and fugu CD4L, carp CD4L also has the conserved p56(lck) tyrosine kinase motif (C-X-C) in the cytoplasmic region. RT-PCR analysis demonstrated that carp CD8 beta and CD4L genes were both expressed predominantly in thymus. The results from this study can be used to understand the evolution of both the CD8 beta and CD4 molecules which can be used as markers for cytotoxic and helper T cells in carp. (c) 2007 Published by Elsevier Ltd. |
| 英文摘要 | Partial cDNA sequences of both CD8 beta and CD4-like (CD4L) genes of common carp (Cyprinus carpio L.) were isolated from thymus cDNA library by the method of suppression subtractive hybridization (SSH). Subsequently the full length cDNAs of carp CD8 and CD4L were obtained by means of 3' RACE and 5' RACE, respectively. The full length cDNA of carp CD8 is 1164 bp and encodes 207 amino acids including a signal peptide region of 24 amino acids, a transmembrane region of 23 amino acids from aa 167 to aa189 and an immunoglobulin V-set from aa 19 to aa 141. Similar to other species CD8 beta s,carp CD8 beta also lacks p56(lck) domain in the cytoplasmic region. The full length cDNA of carp CD4L is 2001 bp and encodes 458 amino acids including four immunoglobulin (Ig)-like domains in the extracellular region, a transmembrane region of 23 amino acids at the C-terminal region from aa 402 to aa 424 and a cytoplasmic tail. Similar to mammalian, avian CD4s and fugu CD4L, carp CD4L also has the conserved p56(lck) tyrosine kinase motif (C-X-C) in the cytoplasmic region. RT-PCR analysis demonstrated that carp CD8 beta and CD4L genes were both expressed predominantly in thymus. The results from this study can be used to understand the evolution of both the CD8 beta and CD4 molecules which can be used as markers for cytotoxic and helper T cells in carp. (c) 2007 Published by Elsevier Ltd. |
| 学科主题 | Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 研究领域[WOS] | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 关键词[WOS] | CLASS-II MHC ; HUMAN-IMMUNODEFICIENCY-VIRUS ; CD8 BETA-CHAIN ; HISTOCOMPATIBILITY COMPLEX-MOLECULES ; CARASSIUS-AURATUS-LANGSDORFII ; BASS DICENTRARCHUS-LABRAX ; GINBUNA CRUCIAN CARP ; T-CELLS ; CORECEPTOR FUNCTION ; ANTIGEN RECEPTOR |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000251472700010 |
| 公开日期 | 2010-10-13 |
| 源URL | [http://ir.ihb.ac.cn/handle/152342/8340]  |
| 专题 | 水生生物研究所_中科院水生所知识产出（2009年前）_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Xiao-Feng,Shang, Na,Hu, Wei,et al. Molecular cloning and characterization of carp (Cyprinus carpio L.) CD8 beta and CD4-like genes[J]. FISH & SHELLFISH IMMUNOLOGY,2007,23(6):1242-1255. |
| APA | Sun, Xiao-Feng,Shang, Na,Hu, Wei,Wang, Ya-Ping,&Guo, Qiong-Lin.(2007).Molecular cloning and characterization of carp (Cyprinus carpio L.) CD8 beta and CD4-like genes.FISH & SHELLFISH IMMUNOLOGY,23(6),1242-1255. |
| MLA | Sun, Xiao-Feng,et al."Molecular cloning and characterization of carp (Cyprinus carpio L.) CD8 beta and CD4-like genes".FISH & SHELLFISH IMMUNOLOGY 23.6(2007):1242-1255. |
入库方式： OAI收割
来源：水生生物研究所
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